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Protein
Homologs
Fitness for 5 genes in
Pseudomonas fluorescens FW300-N1B4
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500 nt
Pf1N1B4_829 and Pf1N1B4_830 overlap by 4 nucleotides
Pf1N1B4_830 and Pf1N1B4_831 are separated by 501 nucleotides
Pf1N1B4_831 and Pf1N1B4_832 are separated by 254 nucleotides
Pf1N1B4_832 and Pf1N1B4_833 are separated by 2 nucleotides
Pf1N1B4_829 - Mobile element protein, at 950,844 to 951,680
_829
Pf1N1B4_830 - Mobile element protein, at 951,677 to 951,979
_830
Pf1N1B4_831 - hypothetical protein, at 952,481 to 952,804
_831
Pf1N1B4_832 - hypothetical protein, at 953,059 to 953,349
_832
Pf1N1B4_833 - PTS system, glucose-specific IIA component / Phosphotransferase system, phosphocarrier protein HPr / Phosphoenolpyruvate-protein phosphotransferase of PTS system (EC 2.7.3.9), at 953,352 to 955,886
_833
Group
Condition
Pf1N1B4
_829
Pf1N1B4
_830
Pf1N1B4
_831
Pf1N1B4
_832
Pf1N1B4
_833
carbon source
D-Trehalose (C)
+0.2
N.D.
N.D.
N.D.
-3.3
nitrogen source
nitrogen source nitrite 0.1mM
+0.3
N.D.
N.D.
N.D.
-0.8
nitrogen source
nitrogen source Ammonium chloride 20mM
-0.3
N.D.
N.D.
N.D.
-0.3
anaerobic
Nitrate reduction
-0.3
N.D.
N.D.
N.D.
-0.3
stress
Aluminum chloride 2.5 mM
+0.0
N.D.
N.D.
N.D.
-0.5
denitrifying
Nitrate reduction, replicate A t2
-0.5
N.D.
N.D.
N.D.
+0.1
denitrifying
Denitrifying in presence of WT Pseudomonas N2-E3; replicate C; timepoint 1
-0.9
N.D.
N.D.
N.D.
+0.7
carbon source
carbon source Sucrose 0.5mM
-0.5
N.D.
N.D.
N.D.
+0.3
stress
nitrate 100 mM
+0.2
N.D.
N.D.
N.D.
-0.4
carbon source
carbon source Sucrose 5mM
+0.3
N.D.
N.D.
N.D.
-0.4
carbon source
L-Arginine (C)
+0.3
N.D.
N.D.
N.D.
-0.3
temperature
LB 30C
+0.3
N.D.
N.D.
N.D.
-0.3
stress
Spectinomycin 0.05 mg/ml
-0.4
N.D.
N.D.
N.D.
+0.4
lb
LB
+0.4
N.D.
N.D.
N.D.
-0.3
carbon source
L-Valine (C)
+0.3
N.D.
N.D.
N.D.
-0.2
carbon source
L-Glutamic (C)
+0.4
N.D.
N.D.
N.D.
-0.3
temperature
LB 37C
+0.5
N.D.
N.D.
N.D.
-0.3
stress
1-ethyl-3-methylimidazolium lysine 5 mM
-0.2
N.D.
N.D.
N.D.
+0.4
carbon source
butyrate (C)
+0.4
N.D.
N.D.
N.D.
-0.2
carbon source
Glycerol (C)
+0.5
N.D.
N.D.
N.D.
-0.2
denitrifying
Denitrifying in presence of WT Pseudomonas N2-E3; replicate B; timepoint 1
-0.2
N.D.
N.D.
N.D.
+0.5
stress
nitrite 10 mM
+0.4
N.D.
N.D.
N.D.
-0.1
carbon source
L-Alanine (C)
+0.5
N.D.
N.D.
N.D.
-0.1
carbon source
Putrescine (C)
+0.5
N.D.
N.D.
N.D.
-0.1
stress
Rifampicin 0.004 mg/ml
-0.2
N.D.
N.D.
N.D.
+0.6
stress
Chloride 300 mM
+0.2
N.D.
N.D.
N.D.
+0.4
nitrogen source
D-Glucosamine Hydrochloride (N)
+0.4
N.D.
N.D.
N.D.
+0.3
carbon source
carbon source Sucrose 0.25mM
+0.5
N.D.
N.D.
N.D.
+0.2
stress
Benzethonium chloride 0.0125 mM
+0.3
N.D.
N.D.
N.D.
+0.5
stress
Doxycycline hyclate 0.0001 mg/ml
+0.4
N.D.
N.D.
N.D.
+0.6
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