Fitness for 5 genes in Pseudomonas fluorescens FW300-N1B4

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500 ntPf1N1B4_772 and Pf1N1B4_773 overlap by 1 nucleotidesPf1N1B4_773 and Pf1N1B4_774 overlap by 4 nucleotidesPf1N1B4_774 and Pf1N1B4_775 are separated by 223 nucleotidesPf1N1B4_775 and Pf1N1B4_776 overlap by 4 nucleotides Pf1N1B4_772 - ABC transporter for L-asparagine and L-glutamate, permease component 1 (from data), at 876,717 to 877,463 _772 Pf1N1B4_773 - ABC transporter for L-asparagine and L-glutamate, permease subunit 2 (from data), at 877,463 to 878,134 _773 Pf1N1B4_774 - ABC transporter for L-asparagine and L-glutamate, ATPase component (from data), at 878,131 to 878,865 _774 Pf1N1B4_775 - Signal transduction histidine kinase regulating C4-dicarboxylate transport system (EC 2.7.13.3), at 879,089 to 880,990 _775 Pf1N1B4_776 - Two component, Sigma-54 Specific, central transcriptional regulator of acidic amino acid uptake, at 880,987 to 882,315 _776
Group Condition Pf1N1B4_772 Pf1N1B4_773 Pf1N1B4_774 Pf1N1B4_775 Pf1N1B4_776
carbon source L-Asparagine (C) -2.2 -1.8 N.D. -1.6 -2.4
carbon source L-Glutamic (C) -1.9 -2.2 N.D. -1.6 -2.1
pH Minimal media pH9 -0.4 -1.0 N.D. -0.6 +0.0
carbon source octanoate (C) -1.2 -0.3 N.D. +0.1 -0.3
stress Benzethonium chloride 0.0125 mM -0.8 -0.3 N.D. +0.3 -0.9
temperature LB 37C -0.3 -0.6 N.D. -0.0 -0.4
nitrogen source nitrogen source nitrite 0.1mM -0.5 +0.0 N.D. +0.0 -0.8
nitrogen source L-Methionine (N) -0.1 -0.1 N.D. +0.1 -1.0
stress D-Cycloserine 0.1 mg/ml -0.3 +0.1 N.D. -0.0 -0.9
pH Minimal media pH9 -0.8 +0.0 N.D. -0.5 +0.3
carbon source L-Arginine (C) -0.2 +0.3 N.D. +0.1 -1.0
carbon source carbon source Sucrose 5mM +0.7 +0.3 N.D. -0.1 -1.4
carbon source carbon source Sucrose 1mM -0.4 +0.6 N.D. -0.4 -0.3
denitrifying Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate B -0.3 -0.4 N.D. +0.2 +0.4
stress Gentamicin 0.001 mg/ml +0.1 -0.3 N.D. -0.2 +0.7
temperature LB 37C -0.9 +0.5 N.D. +0.1 +0.7
nitrogen source nitrogen source nitrite 0.5mM +0.5 +0.1 N.D. +0.2 -0.5
carbon source carbon source Sucrose 0.25mM +0.2 +0.9 N.D. -0.2 -0.3
stress Choline Lysine 6 mM +0.3 -0.1 N.D. -0.3 +0.9
carbon source carbon source Sucrose 0.25mM +0.7 +0.2 N.D. -0.2 +0.2
nitrogen source Cytosine (N) -0.3 +0.2 N.D. +0.1 +0.8
nitrogen source Ammonium chloride (N) -0.2 +0.3 N.D. +0.0 +0.8
stress Fusidic 0.125 mg/ml +0.3 +0.8 N.D. -0.1 +0.0
denitrifying Denitrifying in presence of WT Pseudomonas N2-E3; replicate C; timepoint 1 +0.3 +0.4 N.D. -0.0 +0.5
nitrogen source nitrogen source nitrate 20mM +0.4 +0.6 N.D. +0.2 +0.0
nitrogen source nitrogen source nitrite 0.1mM +0.2 +0.6 N.D. +0.2 +0.2
carbon source carbon source Sucrose 5mM +0.1 +0.9 N.D. -0.1 +0.6
carbon source carbon source Sucrose 20mM +0.0 +0.6 N.D. +0.2 +0.8
carbon source L-Glutamine (C) +0.2 +1.0 N.D. +0.4 +0.4
nitrogen source nitrogen source nitrate 5mM +0.3 +0.6 N.D. +0.1 +0.9
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