Fitness for 5 genes in Pseudomonas fluorescens FW300-N1B4

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500 ntPf1N1B4_694 and Pf1N1B4_695 are separated by 218 nucleotidesPf1N1B4_695 and Pf1N1B4_696 overlap by 4 nucleotidesPf1N1B4_696 and Pf1N1B4_697 are separated by 159 nucleotidesPf1N1B4_697 and Pf1N1B4_698 are separated by 115 nucleotides Pf1N1B4_694 - ABC transporter, periplasmic spermidine putrescine-binding protein PotD (TC 3.A.1.11.1), at 792,211 to 793,314 _694 Pf1N1B4_695 - Aldehyde dehydrogenase (EC 1.2.1.3), at 793,533 to 794,981 _695 Pf1N1B4_696 - Acetolactate synthase, large subunit (EC 2.2.1.6), at 794,978 to 796,615 _696 Pf1N1B4_697 - Glycine cleavage system transcriptional activator, at 796,775 to 797,656 _697 Pf1N1B4_698 - Rrf2-linked NADH-flavin reductase, at 797,772 to 798,386 _698
Group Condition Pf1N1B4_694 Pf1N1B4_695 Pf1N1B4_696 Pf1N1B4_697 Pf1N1B4_698
temperature LB 37C -0.2 -2.6 -0.0 -0.3 -0.0
temperature LB 37C -0.0 -0.4 -0.0 -1.5 -0.4
carbon source carbon source Sucrose 5mM +0.1 -1.1 +0.1 -0.7 -0.5
stress Rifampicin 0.004 mg/ml +0.0 -1.5 +0.3 -0.4 -0.3
stress nitrite 10 mM +0.2 -1.8 -0.3 +0.2 -0.1
stress Spectinomycin 0.05 mg/ml +0.0 -0.4 +0.2 -0.8 -0.7
pH Minimal media pH9 -0.1 -0.3 +0.5 -1.1 -0.6
anaerobic Nitrate reduction +0.2 -1.2 -0.3 +0.2 +0.2
carbon source carbon source Sucrose 0.25mM +0.3 -0.9 -0.2 -0.2 +0.4
pH Minimal media pH9 +0.1 -1.2 +0.0 +0.3 +0.4
stress Choline chloride 200 mM -0.4 -0.5 +0.5 -0.3 +0.3
stress Aluminum chloride 2.5 mM -0.3 +0.6 +0.2 -0.1 -0.7
carbon source L-Isoleucine (C) -0.1 +1.0 -0.7 -0.3 -0.0
denitrifying Denitrifying in presence of WT Pseudomonas N2-E3; replicate C; timepoint 1 +0.4 -0.5 +0.6 -0.1 -0.4
carbon source Tween 20 (C) -0.1 +1.1 -0.6 -0.4 -0.1
stress copper (II) chloride 2.5 mM -0.2 +0.7 +0.4 -0.4 -0.3
stress methylglyoxal 0.008 vol% -0.2 -0.4 +0.5 +0.5 +0.3
carbon source carbon source Sucrose 1mM +0.1 +0.9 +0.4 -0.7 +0.4
nitrogen source nitrogen source nitrite 0.1mM +0.1 +1.1 +0.6 +0.1 -0.8
carbon source D-Ribose (C) -0.1 +1.4 +0.2 +0.1 -0.3
nitrogen source D-Glucosamine Hydrochloride (N) +0.2 -0.4 +1.0 +0.6 +0.1
carbon source pyruvate (C) -0.3 +2.0 -0.1 +0.1 -0.1
carbon source acetate (C) -0.1 +1.7 +0.6 +0.0 -0.5
nitrogen source nitrogen source nitrite 0.1mM +0.2 +1.4 +1.2 -0.0 -0.9
carbon source D-Glucose (C) -0.1 +1.5 +0.5 +0.2 -0.0
carbon source pyruvate (C) +0.1 +2.2 +0.1 -0.1 -0.2
carbon source D-Galacturonic Acid (C) -0.1 +2.3 +0.4 -0.1 -0.3
carbon source acetate (C) -0.1 +2.2 +0.3 +0.1 -0.0
nitrogen source nitrogen source nitrite 0.5mM +0.2 +1.7 +1.0 +0.1 +0.4
nitrogen source nitrogen source nitrite 0.5mM +0.2 +1.7 +1.6 +0.1 +0.1
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