Fitness for 5 genes in Pseudomonas fluorescens FW300-N1B4

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500 ntPf1N1B4_622 and Pf1N1B4_623 are separated by 33 nucleotidesPf1N1B4_623 and Pf1N1B4_624 are separated by 379 nucleotidesPf1N1B4_624 and Pf1N1B4_625 are separated by 95 nucleotidesPf1N1B4_625 and Pf1N1B4_626 are separated by 135 nucleotides Pf1N1B4_622 - L-serine dehydratase (EC 4.3.1.17), at 720,585 to 721,961 _622 Pf1N1B4_623 - Aminomethyltransferase (glycine cleavage system T protein) (EC 2.1.2.10), at 721,995 to 723,119 _623 Pf1N1B4_624 - Cold shock protein CspA, at 723,499 to 723,711 _624 Pf1N1B4_625 - FIG023103: Predicted transmembrane protein, at 723,807 to 724,295 _625 Pf1N1B4_626 - Quinolinate synthetase (EC 2.5.1.72), at 724,431 to 725,489 _626
Group Condition Pf1N1B4_622 Pf1N1B4_623 Pf1N1B4_624 Pf1N1B4_625 Pf1N1B4_626
pH Minimal media pH9 +0.2 +0.8 N.D. -1.1 -4.5
pH Minimal media pH8 +0.0 -0.8 N.D. -0.5 -2.4
denitrifying Nitrate reduction, replicate C +0.2 -0.8 N.D. -1.1 -1.9
denitrifying Nitrate reduction, replicate A +0.1 -0.5 N.D. -1.4 -1.7
denitrifying Nitrate reduction, replicate B +0.1 -0.8 N.D. -0.8 -1.7
denitrifying Denitrifying in presence of WT Pseudomonas N2-E3; replicate C; timepoint 1 +0.1 -2.3 N.D. +0.2 -1.0
carbon source carbon source Sucrose 0.25mM +0.5 -3.4 N.D. -0.3 +0.3
stress Fusidic 0.125 mg/ml -0.1 -0.9 N.D. +0.1 -1.5
carbon source L-Arginine (C) +0.1 +0.5 N.D. -0.3 -2.6
carbon source L-Histidine (C) +0.0 -0.3 N.D. -0.0 -2.2
pH Minimal media pH9 +0.3 +0.4 N.D. +0.2 -3.3
nitrogen source Putrescine (N) +0.0 +0.2 N.D. -0.2 -2.4
nitrogen source nitrate (N) +0.0 +0.0 N.D. -0.2 -2.2
denitrifying Denitrifying in presence of WT Pseudomonas N2-E3; replicate B; timepoint 1 +0.1 -0.4 N.D. -1.1 -0.8
nitrogen source L-Citrulline (N) -0.1 +0.5 N.D. -0.1 -2.3
stress Fusidic 0.0625 mg/ml -0.1 -1.7 N.D. +0.2 -0.3
carbon source Putrescine (C) +0.1 -0.2 N.D. +0.2 -1.9
stress Paraquat dichloride 0.016 mg/mL -0.0 +0.2 N.D. -1.0 -0.9
nitrogen source Ammonium chloride (N) -0.1 +0.7 N.D. -0.5 -1.7
temperature LB 37C +0.0 -1.8 N.D. +0.5 +0.1
carbon source L-Malic (C) -0.0 +0.5 N.D. -0.0 -1.6
nitrogen source D-Alanine (N) +0.3 +0.7 N.D. +0.2 -2.3
carbon source octanoate (C) +0.4 +0.6 N.D. +0.5 -2.4
stress Bacitracin 2 mg/ml +0.0 -2.0 N.D. +0.6 +0.4
carbon source D-Trehalose (C) -0.1 +0.6 N.D. +0.3 -1.8
stress Benzethonium chloride 0.0125 mM -0.0 -1.3 N.D. +0.7 +0.1
carbon source L-Glutamine (C) -0.1 +1.1 N.D. +0.1 -1.4
stress methylglyoxal 0.008 vol% +0.1 +0.9 N.D. -0.9 -0.2
carbon source carbon source Sucrose 1mM +0.2 +0.9 N.D. -1.0 -0.1
nitrogen source L-Serine (N) -0.8 +2.3 N.D. +0.3 -0.3
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