Fitness for 5 genes in Pseudomonas fluorescens FW300-N1B4

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500 ntPf1N1B4_603 and Pf1N1B4_604 are separated by 77 nucleotidesPf1N1B4_604 and Pf1N1B4_605 are separated by 28 nucleotidesPf1N1B4_605 and Pf1N1B4_606 overlap by 69 nucleotidesPf1N1B4_606 and Pf1N1B4_607 are separated by 122 nucleotides Pf1N1B4_603 - Methylglyoxal synthase (EC 4.2.3.3), at 697,008 to 697,472 _603 Pf1N1B4_604 - FIG006045: Sigma factor, ECF subfamily, at 697,550 to 698,068 _604 Pf1N1B4_605 - hypothetical protein, at 698,097 to 698,288 _605 Pf1N1B4_606 - Iron siderophore sensor protein, at 698,220 to 699,128 _606 Pf1N1B4_607 - TonB-dependent hemin , ferrichrome receptor, at 699,251 to 701,812 _607
Group Condition Pf1N1B4_603 Pf1N1B4_604 Pf1N1B4_605 Pf1N1B4_606 Pf1N1B4_607
nitrogen source nitrogen source nitrite 1mM -3.2 +0.3 N.D. -0.6 -0.0
carbon source carbon source Sucrose 0.25mM -0.9 +0.6 N.D. -2.7 -0.1
carbon source carbon source Sucrose 5mM -0.7 +0.1 N.D. -2.4 -0.0
stress Chloride 200 mM -0.4 -0.5 N.D. -2.1 -0.1
nitrogen source nitrogen source Ammonium chloride 20mM -2.2 +0.0 N.D. -0.8 -0.0
nitrogen source nitrogen source nitrite 0.5mM -2.2 -0.3 N.D. -0.2 -0.2
temperature LB 37C +0.2 -0.9 N.D. -2.0 +0.1
nitrogen source nitrogen source nitrite 1mM -2.1 +0.4 N.D. -0.7 +0.0
carbon source carbon source Sucrose 1mM -1.7 +0.1 N.D. -0.5 -0.1
denitrifying Nitrate reduction in presence of wild-type Pseudomonas N2-E3; replicate A -0.3 -0.1 N.D. -1.7 -0.1
nitrogen source nitrogen source Ammonium chloride 20mM -2.2 +0.7 N.D. -0.6 -0.0
pH Minimal media pH8 -1.1 -0.0 N.D. -1.0 +0.0
anaerobic Nitrate reduction -0.9 +0.0 N.D. -1.1 -0.0
stress methylglyoxal 0.008 vol% -0.1 +0.3 N.D. -2.1 -0.1
stress 1-ethyl-3-methylimidazolium lysine 5 mM -0.3 -0.1 N.D. -1.2 -0.3
nitrogen source nitrogen source nitrite 0.1mM -0.1 +0.2 N.D. -1.8 -0.2
pH Minimal media pH7 -2.0 +0.1 N.D. +0.1 +0.0
nitrogen source Ammonium chloride (N) -1.8 +0.3 N.D. -0.3 +0.2
carbon source carbon source Sucrose 5mM -0.8 -1.2 N.D. +0.2 +0.2
anaerobic N2E2wt with N1B4m denitrifying repB +0.0 -0.4 N.D. -1.3 +0.1
pH Minimal media pH7 -1.2 -0.6 N.D. +0.4 +0.1
carbon source carbon source Sucrose 1mM -1.3 -0.2 N.D. +0.4 -0.1
carbon source D-Gluconic Acid (C) -1.4 +0.1 N.D. +0.3 -0.1
nitrogen source nitrogen source nitrate 5mM -0.9 +0.3 N.D. -0.7 +0.1
temperature LB 37C -0.5 +1.1 N.D. -1.8 +0.1
carbon source octanoate (C) +0.7 -0.0 N.D. -1.4 -0.2
carbon source carbon source Sucrose 20mM -0.9 +0.7 N.D. -0.5 -0.1
carbon source carbon source Sucrose 0.25mM -1.2 -0.1 N.D. +0.6 +0.0
carbon source butyrate (C) +0.6 +0.2 N.D. -1.0 -0.1
nitrogen source nitrogen source nitrate 20mM -0.3 +0.9 N.D. -0.5 -0.2
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