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Protein
Homologs
Fitness for 5 genes in
Pseudomonas fluorescens FW300-N1B4
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500 nt
Pf1N1B4_592 and Pf1N1B4_593 are separated by 222 nucleotides
Pf1N1B4_593 and Pf1N1B4_594 are separated by 3 nucleotides
Pf1N1B4_594 and Pf1N1B4_595 overlap by 8 nucleotides
Pf1N1B4_595 and Pf1N1B4_596 are separated by 150 nucleotides
Pf1N1B4_592 - Glucose-selective porin OprB, at 682,990 to 684,336
_592
Pf1N1B4_593 - Glucose ABC transporter, ATP-binding subunit (EC 3.6.3.-), at 684,559 to 685,719
_593
Pf1N1B4_594 - Glucose ABC transport system, inner membrane component 2, at 685,723 to 686,568
_594
Pf1N1B4_595 - Glucose ABC transport system, inner membrane component 1, at 686,561 to 687,505
_595
Pf1N1B4_596 - Glucose ABC transport system, periplasmic sugar-binding protein, at 687,656 to 688,963
_596
Group
Condition
Pf1N1B4
_592
Pf1N1B4
_593
Pf1N1B4
_594
Pf1N1B4
_595
Pf1N1B4
_596
pH
Minimal media pH9
+0.3
-2.9
-2.8
-2.9
-4.0
pH
Minimal media pH9
+0.2
-2.7
-2.9
-2.5
-3.3
pH
Minimal media pH6
+0.1
-2.8
-2.6
-3.1
-2.6
carbon source
D-Mannose (C)
+0.6
-2.2
-1.9
-1.8
-2.3
carbon source
D-Glucose (C)
-0.3
-1.5
-1.3
-2.0
-1.6
nitrogen source
L-Serine (N)
-0.2
-1.2
-1.7
-1.3
-1.6
nitrogen source
L-Arginine (N)
+0.1
-1.7
-1.6
-1.6
-0.9
carbon source
D-Glucose (C)
+0.1
-1.6
-1.2
-1.7
-1.2
nitrogen source
nitrogen source Ammonium chloride 20mM
+0.2
-1.5
-1.3
-1.0
-1.5
carbon source
D-Galactose (C)
+0.1
-1.4
-1.3
-1.5
-0.7
carbon source
D-Galactose (C)
+0.4
-1.4
-1.2
-1.2
-1.1
nitrogen source
nitrogen source Ammonium chloride 20mM
-0.0
-1.0
-1.0
-1.2
-0.8
nitrogen source
L-Histidine (N)
+0.0
-1.0
-0.9
-0.9
-0.5
nitrogen source
nitrogen source nitrate 20mM
-0.0
-1.5
-0.5
-0.9
+0.2
nitrogen source
L-Isoleucine (N)
-0.0
-0.8
-0.2
-0.2
-1.1
nitrogen source
L-Lysine (N)
-0.2
-0.5
-0.6
-0.4
-0.3
nitrogen source
nitrogen source nitrate 20mM
-0.4
+0.3
-0.7
-0.3
-0.4
pH
Minimal media pH8
+0.2
-0.4
-0.5
-0.5
-0.2
nitrogen source
nitrogen source nitrite 0.1mM
+0.1
-0.3
-0.8
-0.5
+0.3
denitrifying
Nitrate reduction, replicate B
+0.2
-0.4
-0.1
-0.1
-0.8
carbon source
D-Fructose (C)
-0.3
-0.6
-0.4
-0.3
+0.4
carbon source
carbon source Sucrose 1mM
+0.1
-1.1
-0.2
-0.1
+0.3
denitrifying
Nitrate reduction, replicate B t2
-0.0
-0.1
-0.6
+0.1
+0.7
carbon source
carbon source Sucrose 0.25mM
-0.2
+0.4
-0.2
-0.2
+0.6
stress
Dimethyl Sulfoxide 5 vol%
+0.1
-0.3
+0.4
+0.2
+0.5
carbon source
carbon source Sucrose 1mM
+0.3
+0.5
+0.1
-0.3
+0.5
carbon source
carbon source Sucrose 0.25mM
+0.5
+0.4
+0.3
-0.2
+0.9
carbon source
Putrescine (C)
+0.0
+0.2
+0.6
+0.6
+0.7
carbon source
casaminos (C)
-0.0
+0.3
+0.6
+0.7
+0.7
nitrogen source
D-Alanine (N)
-0.0
+0.3
+0.7
+0.8
+0.5
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