Fitness for 5 genes in Pseudomonas fluorescens FW300-N1B4

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500 ntPf1N1B4_5856 and Pf1N1B4_5857 are separated by 61 nucleotidesPf1N1B4_5857 and Pf1N1B4_5858 are separated by 340 nucleotidesPf1N1B4_5858 and Pf1N1B4_5859 are separated by 229 nucleotidesPf1N1B4_5859 and Pf1N1B4_5860 are separated by 213 nucleotides Pf1N1B4_5856 - Arginine N-succinyltransferase, alpha subunit (EC 2.3.1.109), at 288,849 to 289,880 _5856 Pf1N1B4_5857 - Arginine N-succinyltransferase (EC 2.3.1.109), at 289,942 to 290,976 _5857 Pf1N1B4_5858 - carboxyphosphonoenolpyruvate phosphonomutase, putative, at 291,317 to 292,186 _5858 Pf1N1B4_5859 - Glutamate carboxypeptidase (EC 3.4.17.11), at 292,416 to 293,657 _5859 Pf1N1B4_5860 - Two-component response regulator, at 293,871 to 294,806 _5860
Group Condition Pf1N1B4_5856 Pf1N1B4_5857 Pf1N1B4_5858 Pf1N1B4_5859 Pf1N1B4_5860
carbon source carbon source Sucrose 0.25mM -0.1 N.D. +0.0 -0.1 -3.5
temperature LB 37C -0.9 N.D. -0.4 -2.2 +0.5
nitrogen source L-Methionine (N) -0.1 N.D. -0.3 -0.9 -1.0
anaerobic N2E2wt with N1B4m denitrifying repA +0.1 N.D. -2.1 +0.3 -0.2
stress D-Cycloserine 0.1 mg/ml -0.4 N.D. -0.6 -0.1 -0.7
anaerobic Nitrate reduction +0.1 N.D. -0.4 -0.6 -0.8
temperature LB 37C +0.2 N.D. -0.7 -0.7 -0.4
denitrifying Denitrifying in presence of WT Pseudomonas N2-E3; replicate C; timepoint 1 -0.3 N.D. +0.3 -0.9 -0.6
pH Minimal media pH9 +0.2 N.D. -0.6 +0.0 -1.1
nitrogen source L-Valine (N) -0.0 N.D. +0.1 +0.1 -1.6
denitrifying Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate A +0.3 N.D. -1.2 -0.3 +0.0
carbon source Putrescine (C) -0.2 N.D. +0.6 -0.1 -1.4
carbon source Cytidine (C) +0.0 N.D. +0.2 +0.4 -1.4
carbon source L-Alanine (C) -0.2 N.D. +0.3 +0.5 -1.4
nitrogen source D-Glucosamine Hydrochloride (N) -0.3 N.D. +0.5 -0.4 -0.7
carbon source L-Lactate (C) -0.2 N.D. -0.7 -0.5 +0.6
stress copper (II) chloride 2.5 mM -0.2 N.D. -0.2 -1.1 +0.7
stress 1-ethyl-3-methylimidazolium lysine 5 mM +0.2 N.D. +0.4 -1.2 +0.1
nitrogen source Putrescine (N) -0.1 N.D. +0.6 +0.3 -1.2
carbon source D-Galacturonic Acid (C) -0.4 N.D. +0.4 +0.6 -0.9
carbon source casaminos (C) +0.1 N.D. +0.5 +0.3 -0.9
carbon source D-Galactose (C) +0.0 N.D. -0.2 -0.8 +0.9
stress Doxycycline hyclate 0.0001 mg/ml -0.1 N.D. +0.5 +0.6 -0.9
nitrogen source Thymine (N) +0.3 N.D. -0.2 +0.8 -0.7
lb LB +0.0 N.D. +0.4 +0.7 -0.6
stress Aluminum chloride 2.5 mM -0.2 N.D. +0.8 +0.8 -0.7
denitrifying Nitrate reduction, replicate C t2 +0.3 N.D. -0.2 -0.3 +1.0
nitrogen source L-Leucine (N) -0.1 N.D. +0.7 +0.7 -0.3
lb LB +0.0 N.D. -0.4 +0.3 +1.1
carbon source carbon source Sucrose 0.25mM -0.2 N.D. +0.8 -0.2 +1.0
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