Fitness for 5 genes in Pseudomonas fluorescens FW300-N1B4

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500 ntPf1N1B4_5847 and Pf1N1B4_5848 are separated by 147 nucleotidesPf1N1B4_5848 and Pf1N1B4_5849 overlap by 1 nucleotidesPf1N1B4_5849 and Pf1N1B4_5850 overlap by 50 nucleotidesPf1N1B4_5850 and Pf1N1B4_5851 are separated by 247 nucleotides Pf1N1B4_5847 - transcriptional activator FtrA, at 280,975 to 281,949 _5847 Pf1N1B4_5848 - Precorrin-6A synthase (EC 2.1.1.152), at 282,097 to 282,852 _5848 Pf1N1B4_5849 - Probable phosphoglycerate mutase, at 282,852 to 283,337 _5849 Pf1N1B4_5850 - hypothetical protein, at 283,288 to 283,509 _5850 Pf1N1B4_5851 - Transcriptional regulator, RpiR family, at 283,757 to 284,611 _5851
Group Condition Pf1N1B4_5847 Pf1N1B4_5848 Pf1N1B4_5849 Pf1N1B4_5850 Pf1N1B4_5851
temperature LB 37C -1.2 -0.3 N.D. N.D. -1.1
stress sodium fluoride 12.5 mM -1.0 -0.1 N.D. N.D. -0.6
carbon source D-Fructose (C) -0.3 -0.7 N.D. N.D. -0.7
anaerobic Nitrate reduction -0.6 +0.1 N.D. N.D. -1.1
denitrifying Nitrate reduction, replicate C -0.4 -0.3 N.D. N.D. -0.7
stress Cobalt chloride 0.3125 mM -0.3 -0.3 N.D. N.D. -0.8
stress nitrate 100 mM +0.0 -0.2 N.D. N.D. -1.2
anaerobic N2E2wt with N1B4m denitrifying repA +0.0 -0.1 N.D. N.D. -1.2
pH Minimal media pH7 -0.4 -0.2 N.D. N.D. -0.6
carbon source L-Malic (C) +0.2 -0.8 N.D. N.D. -0.7
stress Fusidic 0.0625 mg/ml +0.3 -0.4 N.D. N.D. -0.8
carbon source L-Glutamic (C) +0.3 -1.0 N.D. N.D. +0.2
carbon source carbon source Sucrose 0.25mM +0.1 +0.4 N.D. N.D. -0.9
nitrogen source nitrogen source nitrite 0.1mM +0.5 -0.4 N.D. N.D. -0.3
nitrogen source Carnitine Hydrochloride (N) +0.5 -0.8 N.D. N.D. +0.3
carbon source L-Alanine (C) +0.2 -0.7 N.D. N.D. +0.5
carbon source Putrescine (C) +0.3 -0.7 N.D. N.D. +0.5
carbon source L-Asparagine (C) +0.3 -0.5 N.D. N.D. +0.4
carbon source L-Histidine (C) +0.3 -0.8 N.D. N.D. +0.8
nitrogen source L-Isoleucine (N) +0.2 -0.5 N.D. N.D. +0.7
carbon source carbon source Sucrose 0.5mM +0.9 +0.2 N.D. N.D. -0.7
carbon source L-Isoleucine (C) +0.4 -0.4 N.D. N.D. +0.6
stress Dimethyl Sulfoxide 6.5 vol% +0.6 -0.3 N.D. N.D. +0.4
carbon source D-Mannose (C) +0.5 -0.6 N.D. N.D. +0.8
nitrogen source D-Glucosamine Hydrochloride (N) -0.1 -0.3 N.D. N.D. +1.1
carbon source Cytidine (C) +0.6 -0.3 N.D. N.D. +0.5
denitrifying Nitrate reduction in presence of wild-type Pseudomonas N2-E3; replicate A +0.4 -0.3 N.D. N.D. +0.8
carbon source carbon source Sucrose 1mM +1.1 +0.4 N.D. N.D. -0.4
nitrogen source nitrogen source nitrate 20mM +0.9 -0.1 N.D. N.D. +0.5
nitrogen source Cytosine (N) +0.4 +0.3 N.D. N.D. +0.7
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