Fitness for 5 genes in Pseudomonas fluorescens FW300-N1B4

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500 ntPf1N1B4_5753 and Pf1N1B4_5754 are separated by 28 nucleotidesPf1N1B4_5754 and Pf1N1B4_5755 overlap by 7 nucleotidesPf1N1B4_5755 and Pf1N1B4_5756 are separated by 592 nucleotidesPf1N1B4_5756 and Pf1N1B4_5757 overlap by 4 nucleotides Pf1N1B4_5753 - putative membrane protein, at 193,945 to 194,382 _5753 Pf1N1B4_5754 - NnrS protein involved in response to NO, at 194,411 to 195,073 _5754 Pf1N1B4_5755 - NnrS protein involved in response to NO, at 195,067 to 195,600 _5755 Pf1N1B4_5756 - Nitric oxide -responding transcriptional regulator Dnr (Crp/Fnr family), at 196,193 to 196,876 _5756 Pf1N1B4_5757 - hypothetical protein, at 196,873 to 197,067 _5757
Group Condition Pf1N1B4_5753 Pf1N1B4_5754 Pf1N1B4_5755 Pf1N1B4_5756 Pf1N1B4_5757
anaerobic Nitrate reduction -1.0 N.D. N.D. -1.7 N.D.
denitrifying Nitrate reduction, replicate C t2 -0.4 N.D. N.D. -2.0 N.D.
denitrifying Nitrate reduction, replicate C t1 -0.9 N.D. N.D. -1.4 N.D.
denitrifying Nitrate reduction, replicate C t3 -0.4 N.D. N.D. -1.7 N.D.
anaerobic Nitrate reduction -0.3 N.D. N.D. -1.6 N.D.
denitrifying Nitrate reduction, replicate A t2 -0.1 N.D. N.D. -1.2 N.D.
anaerobic Nitrate reduction +0.5 N.D. N.D. -1.8 N.D.
denitrifying Nitrate reduction, replicate B +0.2 N.D. N.D. -1.5 N.D.
lb LB -0.9 N.D. N.D. -0.2 N.D.
denitrifying Nitrate reduction, replicate A t1 +0.0 N.D. N.D. -1.2 N.D.
nitrogen source nitrogen source nitrate 5mM -0.4 N.D. N.D. -0.7 N.D.
stress 1-ethyl-3-methylimidazolium chloride 80 mM -0.7 N.D. N.D. -0.3 N.D.
denitrifying Nitrate reduction in presence of wild-type Pseudomonas N2-E3; replicate A +0.2 N.D. N.D. -1.1 N.D.
stress Doxycycline hyclate 0.0001 mg/ml -1.2 N.D. N.D. +0.4 N.D.
denitrifying Nitrate reduction, replicate C +0.6 N.D. N.D. -1.3 N.D.
carbon source pyruvate (C) -0.8 N.D. N.D. +0.3 N.D.
nitrogen source D-Glucosamine Hydrochloride (N) -1.3 N.D. N.D. +0.9 N.D.
nitrogen source nitrogen source Ammonium chloride 20mM -0.8 N.D. N.D. +0.5 N.D.
denitrifying Denitrifying in presence of WT Pseudomonas N2-E3; replicate B; timepoint 1 +1.2 N.D. N.D. -1.3 N.D.
denitrifying Nitrate reduction, replicate A +0.5 N.D. N.D. -0.5 N.D.
carbon source D-Glucose (C) -0.5 N.D. N.D. +0.7 N.D.
nitrogen source Cytidine (N) +0.8 N.D. N.D. -0.3 N.D.
pH Minimal media pH9 +1.1 N.D. N.D. -0.0 N.D.
temperature LB 37C +1.0 N.D. N.D. +0.3 N.D.
carbon source octanoate (C) +0.6 N.D. N.D. +0.7 N.D.
nitrogen source Adenine (N) +1.0 N.D. N.D. +0.4 N.D.
temperature LB 30C +0.6 N.D. N.D. +0.8 N.D.
nitrogen source Uridine (N) +1.1 N.D. N.D. +0.4 N.D.
temperature LB 37C +0.7 N.D. N.D. +0.9 N.D.
nitrogen source Thymine (N) +1.2 N.D. N.D. +0.7 N.D.
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