Fitness for 5 genes in Pseudomonas fluorescens FW300-N1B4

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500 ntPf1N1B4_5546 and Pf1N1B4_5547 are separated by 37 nucleotidesPf1N1B4_5547 and Pf1N1B4_5548 are separated by 150 nucleotidesPf1N1B4_5548 and Pf1N1B4_5549 are separated by 989 nucleotidesPf1N1B4_5549 and Pf1N1B4_5550 are separated by 322 nucleotides Pf1N1B4_5546 - Chemotaxis response regulator protein-glutamate methylesterase CheB (EC 3.1.1.61), at 317,519 to 318,460 _5546 Pf1N1B4_5547 - hypothetical protein, at 318,498 to 318,779 _5547 Pf1N1B4_5548 - hypothetical protein, at 318,930 to 319,943 _5548 Pf1N1B4_5549 - hypothetical protein, at 320,933 to 321,061 _5549 Pf1N1B4_5550 - Group 2 RNA polymerase sigma factor @ Cyanobacteria-specific RpoD-like sigma factor, type-7, at 321,384 to 322,937 _5550
Group Condition Pf1N1B4_5546 Pf1N1B4_5547 Pf1N1B4_5548 Pf1N1B4_5549 Pf1N1B4_5550
carbon source carbon source Sucrose 0.25mM +0.0 N.D. +0.1 -1.5 -0.1
carbon source carbon source Sucrose 1mM -0.3 N.D. +0.0 -1.3 +0.4
carbon source octanoate (C) +0.0 N.D. -0.1 -0.3 -0.8
carbon source carbon source Sucrose 0.25mM -0.5 N.D. -0.1 -0.5 -0.0
carbon source D,L-Lactate (C) -0.0 N.D. -0.5 -0.4 -0.0
nitrogen source nitrogen source nitrite 0.1mM -0.0 N.D. -0.3 -0.8 +0.2
carbon source L-Arabinose (C) +0.0 N.D. +0.4 -0.7 -0.5
carbon source L-Arabinose (C) -0.1 N.D. -0.4 -0.5 +0.2
anaerobic N2E2wt with N1B4m denitrifying repA -0.1 N.D. +0.5 -0.8 -0.2
carbon source carbon source Sucrose 5mM -0.3 N.D. -0.2 -0.5 +0.3
nitrogen source nitrogen source Ammonium chloride 20mM +0.2 N.D. +0.1 -0.8 -0.1
carbon source L-Lactate (C) -0.2 N.D. +0.2 -0.6 -0.0
nitrogen source nitrate (N) +0.3 N.D. +0.1 -0.7 -0.2
nitrogen source Cytosine (N) +0.3 N.D. +0.3 -1.1 +0.1
stress Carbenicillin 2 mg/ml -0.1 N.D. +0.5 -0.6 -0.1
temperature LB 37C -0.1 N.D. +0.0 -0.5 +0.4
nitrogen source Cytidine (N) +0.1 N.D. +0.5 -0.5 -0.1
nitrogen source Ammonium chloride (N) +0.3 N.D. +0.3 -0.5 -0.0
nitrogen source nitrogen source nitrite 0.5mM +0.3 N.D. -0.2 -0.2 +0.3
stress Cisplatin 0.006 mg/ml -0.4 N.D. -0.2 +0.3 +0.5
carbon source L-Alanine (C) +0.1 N.D. -0.3 +0.4 +0.2
carbon source carbon source Sucrose 20mM +0.1 N.D. -0.1 +0.6 +0.3
pH Minimal media pH9 -0.3 N.D. +0.3 +0.6 +0.4
stress Cisplatin 0.006 mg/ml -0.0 N.D. +0.0 +0.7 +0.3
lb LB +0.2 N.D. +0.4 +0.4 +0.2
carbon source carbon source Sucrose 5mM +0.1 N.D. +0.1 +0.5 +0.4
denitrifying Denitrifying in presence of WT Pseudomonas N2-E3; replicate C; timepoint 1 +0.2 N.D. +0.5 -0.1 +0.6
stress Sisomicin 0.001 mg/ml +0.4 N.D. +0.5 -0.0 +0.3
denitrifying Nitrate reduction, replicate C -0.0 N.D. +0.2 +0.8 +0.3
stress Fusidic 0.125 mg/ml -0.0 N.D. +0.6 +1.1 -0.4
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