Fitness for 5 genes in Pseudomonas fluorescens FW300-N1B4

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500 ntPf1N1B4_5512 and Pf1N1B4_5513 are separated by 75 nucleotidesPf1N1B4_5513 and Pf1N1B4_5514 are separated by 69 nucleotidesPf1N1B4_5514 and Pf1N1B4_5515 are separated by 50 nucleotidesPf1N1B4_5515 and Pf1N1B4_5516 are separated by 802 nucleotides Pf1N1B4_5512 - hypothetical protein, at 261,098 to 261,244 _5512 Pf1N1B4_5513 - hypothetical protein, at 261,320 to 261,517 _5513 Pf1N1B4_5514 - RecA protein, at 261,587 to 262,636 _5514 Pf1N1B4_5515 - ATP-dependent DNA helicase pcrA (EC 3.6.1.-), at 262,687 to 266,067 _5515 Pf1N1B4_5516 - hypothetical protein, at 266,870 to 267,001 _5516
Group Condition Pf1N1B4_5512 Pf1N1B4_5513 Pf1N1B4_5514 Pf1N1B4_5515 Pf1N1B4_5516
temperature LB 37C -3.8 -1.5 +0.1 +0.1 N.D.
carbon source D-Glucose (C) -1.3 -1.3 -0.6 -0.1 N.D.
carbon source D-Galactose (C) -1.2 -0.9 -0.0 +0.0 N.D.
carbon source L-Lactate (C) -1.1 -0.8 -0.1 +0.0 N.D.
denitrifying Denitrifying in presence of WT Pseudomonas N2-E3; replicate C; timepoint 1 -0.8 -1.3 -0.3 +0.4 N.D.
carbon source D,L-Lactate (C) -1.8 -0.1 -0.0 +0.2 N.D.
carbon source carbon source Sucrose 0.25mM -0.6 -1.2 +0.0 +0.2 N.D.
stress Paraquat dichloride 0.016 mg/mL -1.0 -0.1 -0.2 -0.2 N.D.
carbon source D-Fructose (C) -1.3 -0.6 +0.2 +0.3 N.D.
carbon source D-Fructose (C) -1.6 +0.4 -0.2 +0.0 N.D.
lb LB -1.5 +0.4 -0.0 +0.1 N.D.
stress Carbenicillin 2 mg/ml -1.1 -0.1 +0.2 +0.2 N.D.
stress sodium fluoride 12.5 mM -1.2 +0.2 -0.0 +0.2 N.D.
carbon source D-Gluconic Acid (C) -1.1 +0.3 -0.1 +0.2 N.D.
denitrifying Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate A -0.7 +0.3 -0.5 +0.1 N.D.
nitrogen source L-Lysine (N) +0.5 -0.5 -0.4 -0.1 N.D.
stress Choline Lysine 6 mM +0.0 -0.8 +0.3 +0.4 N.D.
carbon source carbon source Sucrose 5mM +0.5 -0.8 +0.2 +0.2 N.D.
stress Benzethonium chloride 0.0125 mM +0.7 +0.1 -0.4 -0.4 N.D.
stress Dimethyl Sulfoxide 6.5 vol% +0.9 -0.4 -0.3 +0.1 N.D.
carbon source carbon source Sucrose 1mM +1.3 -0.8 -0.1 +0.1 N.D.
nitrogen source D-Glucosamine Hydrochloride (N) +0.9 -0.6 +0.2 +0.0 N.D.
nitrogen source L-Leucine (N) +0.6 +0.5 -0.5 -0.1 N.D.
carbon source D-Trehalose (C) +0.8 +0.3 -0.4 -0.1 N.D.
pH Minimal media pH9 +0.7 +0.5 -0.5 -0.1 N.D.
carbon source L-Isoleucine (C) +0.9 +0.3 -0.4 +0.2 N.D.
stress Aluminum chloride 2.5 mM +0.9 +0.2 -0.2 +0.1 N.D.
nitrogen source nitrogen source nitrite 0.1mM +1.8 -0.2 -0.1 -0.2 N.D.
carbon source m-Inositol (C) +1.4 +0.2 -0.2 -0.0 N.D.
carbon source octanoate (C) +0.9 +0.4 +0.3 +0.3 N.D.
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