Fitness for 5 genes in Pseudomonas fluorescens FW300-N1B4

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500 ntPf1N1B4_5498 and Pf1N1B4_5499 are separated by 367 nucleotidesPf1N1B4_5499 and Pf1N1B4_5500 are separated by 177 nucleotidesPf1N1B4_5500 and Pf1N1B4_5501 are separated by 209 nucleotidesPf1N1B4_5501 and Pf1N1B4_5502 overlap by 35 nucleotides Pf1N1B4_5498 - hypothetical protein, at 247,310 to 247,522 _5498 Pf1N1B4_5499 - hypothetical protein, at 247,890 to 249,695 _5499 Pf1N1B4_5500 - hypothetical protein, at 249,873 to 250,862 _5500 Pf1N1B4_5501 - hypothetical protein, at 251,072 to 251,413 _5501 Pf1N1B4_5502 - hypothetical protein, at 251,379 to 252,542 _5502
Group Condition Pf1N1B4_5498 Pf1N1B4_5499 Pf1N1B4_5500 Pf1N1B4_5501 Pf1N1B4_5502
nitrogen source nitrogen source nitrite 0.1mM -1.8 -0.0 +0.3 -1.3 -0.4
nitrogen source nitrogen source nitrate 20mM -1.4 +0.0 -0.1 -1.1 +0.2
stress Doxycycline hyclate 0.0001 mg/ml -1.9 -0.2 -0.1 -0.3 +0.1
denitrifying Nitrate reduction, replicate B -1.4 -0.1 -0.1 -0.6 -0.1
stress methylglyoxal 0.008 vol% -1.3 -0.1 -0.3 -0.6 +0.1
nitrogen source Putrescine (N) -2.4 -0.0 +0.1 +0.1 +0.2
carbon source D-Glucose (C) -2.0 -0.0 +0.1 -0.2 +0.1
stress sodium fluoride 12.5 mM -0.9 -0.1 -0.3 -0.3 -0.3
stress 1-ethyl-3-methylimidazolium lysine 5 mM -1.5 -0.1 -0.1 -0.1 -0.1
stress methylglyoxal 0.004 vol% -1.4 -0.1 -0.1 -0.1 -0.1
carbon source L-Lactate (C) -1.2 -0.0 +0.0 -0.5 -0.1
pH Minimal media pH9 -1.1 +0.1 +0.1 -0.6 +0.0
denitrifying Denitrifying in presence of WT Pseudomonas N2-E3; replicate C; timepoint 1 -1.8 -0.0 +0.2 -0.0 +0.2
nitrogen source nitrogen source Ammonium chloride 20mM -1.0 -0.0 +0.2 -0.6 -0.1
stress Gentamicin 0.001 mg/ml -1.2 -0.2 +0.0 -0.2 +0.2
anaerobic N2E2wt with N1B4m denitrifying repB -1.1 +0.1 -0.4 +0.3 -0.3
stress Rifampicin 0.004 mg/ml -1.5 -0.1 +0.3 -0.4 +0.4
carbon source carbon source Sucrose 5mM -1.4 -0.2 +0.2 +0.0 +0.2
stress nitrate 100 mM -1.3 -0.2 +0.2 +0.1 +0.2
stress Nalidixic 0.004 mg/ml -0.7 -0.1 +0.4 -0.5 -0.0
stress Benzethonium chloride 0.0125 mM +0.5 -0.3 -0.4 -0.8 +0.1
nophagecontrol TIme6-nophage -0.8 +0.3 +0.2 -0.4 +0.1
carbon source carbon source Sucrose 1mM +1.0 +0.2 -0.4 -1.2 -0.0
carbon source D-Ribose (C) +0.7 +0.0 -0.2 -0.4 -0.4
carbon source octanoate (C) -1.1 -0.1 +0.7 +0.2 +0.1
carbon source m-Inositol (C) -1.0 -0.0 +0.4 +0.4 -0.0
nitrogen source Cytidine (N) +0.8 +0.0 +0.2 -0.9 -0.3
nitrogen source D-Glucosamine Hydrochloride (N) +1.2 -0.4 -0.1 -0.7 +0.1
carbon source carbon source Sucrose 1mM +0.9 +0.6 -0.6 -0.8 +0.3
stress Cisplatin 0.006 mg/ml +0.7 +0.1 +1.2 +0.0 +0.3
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