Fitness for 5 genes in Pseudomonas fluorescens FW300-N1B4

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500 ntPf1N1B4_5449 and Pf1N1B4_5450 overlap by 25 nucleotidesPf1N1B4_5450 and Pf1N1B4_5451 are separated by 1382 nucleotidesPf1N1B4_5451 and Pf1N1B4_5452 are separated by 1247 nucleotidesPf1N1B4_5452 and Pf1N1B4_5453 are separated by 30 nucleotides Pf1N1B4_5449 - Error-prone repair protein UmuD, at 194,820 to 195,131 _5449 Pf1N1B4_5450 - Error-prone, lesion bypass DNA polymerase V (UmuC), at 195,107 to 196,402 _5450 Pf1N1B4_5451 - hypothetical protein, at 197,785 to 198,030 _5451 Pf1N1B4_5452 - hypothetical protein, at 199,278 to 199,538 _5452 Pf1N1B4_5453 - hypothetical protein, at 199,569 to 199,898 _5453
Group Condition Pf1N1B4_5449 Pf1N1B4_5450 Pf1N1B4_5451 Pf1N1B4_5452 Pf1N1B4_5453
carbon source carbon source Sucrose 0.5mM N.D. -0.0 -0.7 -0.9 -1.1
carbon source L-Arabinose (C) N.D. -0.0 -1.7 -0.8 -0.1
stress Cisplatin 0.006 mg/ml N.D. -1.6 -0.1 -0.1 -0.4
carbon source D-Gluconic Acid (C) N.D. -0.2 -1.2 -0.3 -0.3
carbon source carbon source Sucrose 0.25mM N.D. -0.1 +0.2 -0.6 -1.7
stress Cisplatin 0.006 mg/ml N.D. -1.7 +0.2 +0.0 -0.6
stress sodium fluoride 12.5 mM N.D. -0.5 -0.7 -0.5 -0.3
carbon source D-Gluconic Acid (C) N.D. -0.1 -0.6 -0.4 -0.7
carbon source carbon source Sucrose 0.25mM N.D. -0.3 -0.9 +0.3 -0.8
nitrogen source L-Isoleucine (N) N.D. -0.0 -1.3 -0.1 -0.2
carbon source acetate (C) N.D. +0.0 -0.6 -0.3 -0.7
stress Cholin acetate 10 mM N.D. -0.1 -0.5 -0.1 -0.8
stress Benzethonium chloride 0.0125 mM N.D. +0.1 -1.0 +0.3 -0.8
carbon source D-Lactate (C) N.D. +0.1 -0.4 -0.6 -0.4
temperature LB 37C N.D. -0.0 -1.5 -0.3 +0.8
stress acetate 20 mM N.D. -0.5 +0.3 -0.0 -0.9
stress Fusidic 0.125 mg/ml N.D. -0.2 -0.9 +0.3 -0.2
carbon source D-Ribose (C) N.D. +0.2 -0.4 -0.7 +0.4
carbon source D-Fructose (C) N.D. +0.0 +0.5 -0.5 -0.5
nitrogen source L-Methionine (N) N.D. -0.1 +0.6 -0.1 -0.9
temperature LB 37C N.D. +0.4 -0.2 +0.2 -0.8
stress Choline chloride 200 mM N.D. -0.0 +0.6 -0.2 -0.7
nitrogen source nitrogen source nitrate 20mM N.D. +0.3 +0.4 -0.3 -0.5
anaerobic N2E2wt with N1B4m denitrifying repA N.D. +0.0 +0.5 -1.0 +0.3
carbon source a-Ketoglutaric (C) N.D. -0.1 +0.5 -0.6 +0.4
carbon source D-Lactate (C) N.D. +0.0 +0.6 -0.5 +0.5
stress Tetracycline 0.0004 mg/ml N.D. +0.1 +0.8 +0.3 -0.3
denitrifying Denitrifying in presence of WT Pseudomonas N2-E3; replicate C; timepoint 1 N.D. -0.1 +1.3 -0.1 +0.5
nitrogen source nitrate (N) N.D. +0.2 +0.0 +0.4 +0.9
nitrogen source Cytidine (N) N.D. +0.0 +0.5 +0.3 +0.9
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