Fitness for 5 genes in Pseudomonas fluorescens FW300-N1B4

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500 ntPf1N1B4_5249 and Pf1N1B4_5250 are separated by 353 nucleotidesPf1N1B4_5250 and Pf1N1B4_5251 are separated by 522 nucleotidesPf1N1B4_5251 and Pf1N1B4_5252 are separated by 169 nucleotidesPf1N1B4_5252 and Pf1N1B4_5253 are separated by 11 nucleotides Pf1N1B4_5249 - Outer membrane protein A precursor, at 7,856 to 8,512 _5249 Pf1N1B4_5250 - hypothetical protein, at 8,866 to 8,994 _5250 Pf1N1B4_5251 - hypothetical protein, at 9,517 to 10,368 _5251 Pf1N1B4_5252 - hypothetical protein, at 10,538 to 11,272 _5252 Pf1N1B4_5253 - hypothetical protein, at 11,284 to 12,045 _5253
Group Condition Pf1N1B4_5249 Pf1N1B4_5250 Pf1N1B4_5251 Pf1N1B4_5252 Pf1N1B4_5253
nitrogen source nitrogen source nitrite 0.1mM -1.3 N.D. -0.4 -0.5 -0.1
carbon source carbon source Sucrose 0.5mM -1.0 N.D. -0.7 -0.5 -0.2
nitrogen source Uridine (N) -0.6 N.D. -0.5 -0.4 -0.2
temperature LB 37C -1.3 N.D. -0.7 +0.1 +0.5
carbon source carbon source Sucrose 0.25mM -0.4 N.D. -0.3 -1.1 +0.4
carbon source carbon source Sucrose 5mM -0.6 N.D. +0.3 -0.3 -0.6
stress Fusidic 0.125 mg/ml -1.0 N.D. -0.2 -0.0 -0.1
carbon source D-Trehalose (C) -0.5 N.D. -0.3 -0.1 -0.4
anaerobic N2E2wt with N1B4m denitrifying repA -0.4 N.D. -0.7 +0.3 -0.5
carbon source casaminos (C) -0.8 N.D. -0.3 +0.1 -0.1
carbon source carbon source Sucrose 0.25mM +0.1 N.D. -0.7 -0.3 +0.1
nitrogen source nitrogen source nitrite 0.1mM -0.5 N.D. -0.0 -0.5 +0.2
pH Minimal media pH9 -1.0 N.D. -0.1 +0.3 +0.0
stress Benzethonium chloride 0.0125 mM -0.1 N.D. +0.5 -0.3 -0.6
stress Carbenicillin 2 mg/ml -0.3 N.D. +0.4 +0.0 -0.6
carbon source D-Glucose (C) -0.8 N.D. +0.1 +0.3 +0.1
stress Fusidic 0.0625 mg/ml +0.6 N.D. -0.5 -0.3 -0.0
nitrogen source nitrogen source nitrate 20mM +0.6 N.D. -0.8 +0.4 -0.1
stress 1-ethyl-3-methylimidazolium lysine 5 mM -0.4 N.D. -0.3 +0.3 +0.5
denitrifying Nitrate reduction, replicate B t1 -0.7 N.D. -0.2 +0.6 +0.5
stress Choline Lysine 6 mM -0.3 N.D. -0.2 +0.5 +0.5
stress Cobalt chloride 0.3125 mM +0.6 N.D. +0.3 -0.2 -0.1
nitrogen source nitrogen source nitrate 20mM +0.5 N.D. -0.3 +0.1 +0.4
carbon source carbon source Sucrose 5mM +1.7 N.D. -0.7 -0.2 -0.0
stress 1-ethyl-3-methylimidazolium chloride 80 mM +0.2 N.D. -0.3 +0.4 +0.6
anaerobic Nitrate reduction +0.7 N.D. +0.2 -0.1 +0.4
stress Chloride 300 mM +0.5 N.D. +0.1 +0.4 +0.3
lb LB +0.3 N.D. +0.3 +0.3 +0.4
stress nitrate 100 mM +0.4 N.D. -0.0 +0.7 +0.4
denitrifying Denitrifying in presence of WT Pseudomonas N2-E3; replicate C; timepoint 1 +0.9 N.D. +0.8 +0.2 +0.7
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