Fitness for 5 genes in Pseudomonas fluorescens FW300-N1B4

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500 ntPf1N1B4_5070 and Pf1N1B4_5071 are separated by 233 nucleotidesPf1N1B4_5071 and Pf1N1B4_5072 are separated by 90 nucleotidesPf1N1B4_5072 and Pf1N1B4_5073 are separated by 4 nucleotidesPf1N1B4_5073 and Pf1N1B4_5074 are separated by 115 nucleotides Pf1N1B4_5070 - Aspartate aminotransferase (EC 2.6.1.1), at 5,615,002 to 5,616,216 _5070 Pf1N1B4_5071 - FIG00959535: hypothetical protein, at 5,616,450 to 5,617,673 _5071 Pf1N1B4_5072 - Glycerol-3-phosphate regulon repressor, at 5,617,764 to 5,618,582 _5072 Pf1N1B4_5073 - Hydroxypyruvate isomerase (EC 5.3.1.22), at 5,618,587 to 5,619,369 _5073 Pf1N1B4_5074 - Gluconate permease, at 5,619,485 to 5,620,897 _5074
Group Condition Pf1N1B4_5070 Pf1N1B4_5071 Pf1N1B4_5072 Pf1N1B4_5073 Pf1N1B4_5074
stress 1-ethyl-3-methylimidazolium chloride 80 mM -0.1 -1.1 -1.1 -0.1 -1.2
stress methylglyoxal 0.008 vol% -0.1 -1.1 +0.4 -0.9 -1.4
carbon source carbon source Sucrose 1mM -0.3 -1.2 -1.6 +0.6 -0.5
carbon source L-Arabinose (C) -0.3 -0.9 -0.6 +0.3 -0.4
stress Cisplatin 0.006 mg/ml +0.3 +0.1 -0.8 -1.0 -0.5
nitrogen source L-Methionine (N) -0.1 -0.0 -0.7 +0.3 -1.4
denitrifying Denitrifying in presence of WT Pseudomonas N2-E3; replicate C; timepoint 1 +0.2 -1.0 -0.5 +0.4 -1.0
nitrogen source Thymine (N) +0.2 +0.3 -0.6 -0.5 -1.0
pH Minimal media pH9 -0.3 -0.7 -0.3 +0.4 -0.8
nitrogen source Uridine (N) +0.2 +0.9 -0.7 -1.0 -0.7
nitrogen source Cytosine (N) +0.1 +1.0 -0.4 -0.8 -1.2
carbon source carbon source Sucrose 5mM +0.4 -1.4 +0.3 +0.0 -0.5
nitrogen source D-Glucosamine Hydrochloride (N) -0.1 -0.8 +0.3 -0.7 +0.3
anaerobic Nitrate reduction -0.1 -1.0 +0.5 -0.6 +0.2
carbon source L-Arginine (C) +0.2 +0.4 -0.7 +0.0 -1.0
lb LB +0.3 -0.9 -0.2 +0.6 -0.6
carbon source L-Arabinose (C) +0.1 -1.3 -0.8 +0.8 +0.4
denitrifying Nitrate reduction, replicate C t3 -0.0 -0.7 +0.4 +0.4 -0.8
phagefrs Time6-FRS +0.2 -0.6 -0.0 +0.8 -0.7
nitrogen source nitrogen source Ammonium chloride 20mM -0.4 +0.8 -0.4 +0.4 -0.4
carbon source Uridine (C) +0.2 -0.6 -0.5 +0.9 +0.1
temperature LB 37C +0.8 -1.3 +0.2 +1.1 -0.3
stress Spectinomycin 0.025 mg/ml +0.1 -0.8 +0.6 +0.2 +0.5
stress Benzethonium chloride 0.0125 mM +0.5 -1.5 +0.2 +0.5 +1.0
carbon source carbon source Sucrose 0.25mM +0.5 -0.6 +0.7 +0.4 -0.2
nitrogen source nitrogen source nitrate 20mM -0.1 +0.4 +0.4 +0.8 -0.6
stress Doxycycline hyclate 0.0001 mg/ml +0.2 +0.4 -1.0 +0.4 +1.1
carbon source octanoate (C) +1.0 -0.3 -0.2 +0.4 +0.5
temperature LB 37C +0.4 +0.0 +0.9 -0.4 +0.9
stress Rifampicin 0.004 mg/ml +0.3 +0.1 +0.8 +0.4 +0.7
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