Fitness for 5 genes in Pseudomonas fluorescens FW300-N1B4

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500 ntPf1N1B4_5066 and Pf1N1B4_5067 are separated by 105 nucleotidesPf1N1B4_5067 and Pf1N1B4_5068 overlap by 1 nucleotidesPf1N1B4_5068 and Pf1N1B4_5069 are separated by 202 nucleotidesPf1N1B4_5069 and Pf1N1B4_5070 overlap by 4 nucleotides Pf1N1B4_5066 - Transcriptional regulator, LysR family, at 5,612,042 to 5,612,935 _5066 Pf1N1B4_5067 - PUTATIVE TRANSFERASE PROTEIN( EC:2.- ), at 5,613,041 to 5,613,706 _5067 Pf1N1B4_5068 - FIG00974267: hypothetical protein, at 5,613,706 to 5,614,368 _5068 Pf1N1B4_5069 - Demethylmenaquinone methyltransferase, at 5,614,571 to 5,615,005 _5069 Pf1N1B4_5070 - Aspartate aminotransferase (EC 2.6.1.1), at 5,615,002 to 5,616,216 _5070
Group Condition Pf1N1B4_5066 Pf1N1B4_5067 Pf1N1B4_5068 Pf1N1B4_5069 Pf1N1B4_5070
stress Gentamicin 0.001 mg/ml N.D. -1.3 N.D. N.D. -0.1
denitrifying Denitrifying in presence of WT Pseudomonas N2-E3; replicate C; timepoint 1 N.D. -1.5 N.D. N.D. +0.2
anaerobic Nitrate reduction N.D. -0.7 N.D. N.D. -0.6
nitrogen source nitrogen source nitrite 0.1mM N.D. -0.4 N.D. N.D. -0.5
carbon source D-Gluconic Acid (C) N.D. -0.9 N.D. N.D. +0.1
carbon source D-Galacturonic Acid (C) N.D. -0.9 N.D. N.D. +0.1
carbon source carbon source Sucrose 0.25mM N.D. -0.8 N.D. N.D. +0.1
carbon source D,L-Lactate (C) N.D. -0.8 N.D. N.D. +0.1
stress sodium fluoride 12.5 mM N.D. -0.7 N.D. N.D. +0.1
anaerobic N2E2wt with N1B4m denitrifying repB N.D. -0.8 N.D. N.D. +0.2
pH Minimal media pH9 N.D. -0.6 N.D. N.D. +0.1
carbon source pyruvate (C) N.D. -0.6 N.D. N.D. +0.2
nitrogen source nitrogen source nitrate 5mM N.D. -0.7 N.D. N.D. +0.3
temperature LB 37C N.D. -1.1 N.D. N.D. +0.8
carbon source Fumarate (C) N.D. -0.7 N.D. N.D. +0.4
lb LB N.D. -0.6 N.D. N.D. +0.3
carbon source carbon source Sucrose 5mM N.D. -0.6 N.D. N.D. +0.4
nitrogen source L-Histidine (N) N.D. -0.5 N.D. N.D. +0.3
stress Chlorite 1 mM N.D. -0.4 N.D. N.D. +0.3
carbon source carbon source Sucrose 0.5mM N.D. -0.3 N.D. N.D. +0.5
denitrifying Nitrate reduction, replicate A N.D. +0.6 N.D. N.D. -0.3
denitrifying Nitrate reduction, replicate C t1 N.D. +0.9 N.D. N.D. -0.1
denitrifying Nitrate reduction, replicate C t2 N.D. +0.7 N.D. N.D. +0.1
nitrogen source nitrate (N) N.D. +0.7 N.D. N.D. +0.2
denitrifying Nitrate reduction, replicate B t1 N.D. +1.1 N.D. N.D. -0.2
denitrifying Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate B N.D. +0.5 N.D. N.D. +0.5
denitrifying Nitrate reduction, replicate A t2 N.D. +1.2 N.D. N.D. -0.1
carbon source octanoate (C) N.D. +0.2 N.D. N.D. +1.0
denitrifying Nitrate reduction, replicate B t2 N.D. +1.1 N.D. N.D. +0.2
denitrifying Nitrate reduction, replicate A t1 N.D. +1.0 N.D. N.D. +0.6
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