Fitness for 5 genes in Pseudomonas fluorescens FW300-N1B4

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500 ntPf1N1B4_501 and Pf1N1B4_502 are separated by 57 nucleotidesPf1N1B4_502 and Pf1N1B4_503 are separated by 2 nucleotidesPf1N1B4_503 and Pf1N1B4_504 are separated by 26 nucleotidesPf1N1B4_504 and Pf1N1B4_505 are separated by 465 nucleotides Pf1N1B4_501 - Flp pilus assembly protein RcpC/CpaB, at 593,362 to 594,132 _501 Pf1N1B4_502 - Predicted ATPase with chaperone activity, associated with Flp pilus assembly, at 594,190 to 595,524 _502 Pf1N1B4_503 - Type IV prepilin peptidase TadV/CpaA, at 595,527 to 596,057 _503 Pf1N1B4_504 - Flp pilus assembly protein, pilin Flp, at 596,084 to 596,275 _504 Pf1N1B4_505 - hypothetical protein, at 596,741 to 596,926 _505
Group Condition Pf1N1B4_501 Pf1N1B4_502 Pf1N1B4_503 Pf1N1B4_504 Pf1N1B4_505
temperature LB 37C -3.6 +0.3 +0.1 N.D. N.D.
carbon source carbon source Sucrose 1mM -2.3 +0.3 -0.5 N.D. N.D.
stress Rifampicin 0.004 mg/ml -1.8 -0.1 -0.1 N.D. N.D.
phagefrs Time6-FRS -1.7 -0.2 +0.1 N.D. N.D.
stress benzoic 0.58 mg/ml -1.3 -0.1 -0.3 N.D. N.D.
stress Bacitracin 2 mg/ml -1.2 -0.3 -0.1 N.D. N.D.
denitrifying Denitrifying in presence of WT Pseudomonas N2-E3; replicate B; timepoint 1 -1.8 +0.0 +0.2 N.D. N.D.
temperature LB 10C -1.3 -0.0 -0.3 N.D. N.D.
carbon source carbon source Sucrose 1mM -0.9 -0.2 -0.2 N.D. N.D.
carbon source acetate (C) -1.3 -0.1 +0.1 N.D. N.D.
pH Minimal media pH9 -0.4 -0.5 -0.5 N.D. N.D.
carbon source carbon source Sucrose 0.25mM -0.7 -0.4 -0.2 N.D. N.D.
carbon source D-Lactate (C) -1.3 -0.2 +0.1 N.D. N.D.
nitrogen source Cytidine (N) -0.6 -0.5 -0.2 N.D. N.D.
nitrogen source L-Methionine (N) -1.1 +0.1 -0.2 N.D. N.D.
stress copper (II) chloride 2.5 mM -1.0 -0.2 +0.1 N.D. N.D.
nitrogen source nitrogen source nitrite 1mM -1.0 +0.2 -0.3 N.D. N.D.
stress nitrite 10 mM -1.1 +0.0 -0.0 N.D. N.D.
carbon source D-Galactose (C) -1.2 -0.0 +0.3 N.D. N.D.
carbon source D-Fructose (C) -1.0 -0.1 +0.3 N.D. N.D.
anaerobic N2E2wt with N1B4m denitrifying repA -0.8 -0.0 +0.3 N.D. N.D.
stress Doxycycline hyclate 0.0001 mg/ml +0.8 -0.4 -0.7 N.D. N.D.
lb LB +0.5 -0.0 -0.7 N.D. N.D.
stress 5-Hydroxymethylfurfural 2.5 mM +0.6 -0.0 -0.4 N.D. N.D.
temperature LB 37C +0.9 +0.1 -0.7 N.D. N.D.
stress methylglyoxal 0.008 vol% +0.8 -0.0 -0.3 N.D. N.D.
denitrifying Nitrate reduction, replicate C t3 +0.6 -0.3 +0.2 N.D. N.D.
nitrogen source Carnitine Hydrochloride (N) +1.0 +0.0 -0.2 N.D. N.D.
stress D-Cycloserine 0.1 mg/ml +1.0 +0.2 -0.3 N.D. N.D.
denitrifying Nitrate reduction, replicate A +0.7 +0.1 +0.3 N.D. N.D.
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