Fitness for 5 genes in Pseudomonas fluorescens FW300-N1B4

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500 ntPf1N1B4_4890 and Pf1N1B4_4891 are separated by 122 nucleotidesPf1N1B4_4891 and Pf1N1B4_4892 are separated by 277 nucleotidesPf1N1B4_4892 and Pf1N1B4_4893 are separated by 51 nucleotidesPf1N1B4_4893 and Pf1N1B4_4894 are separated by 31 nucleotides Pf1N1B4_4890 - Cystathionine gamma-lyase (EC 4.4.1.1), at 5,412,449 to 5,413,630 _4890 Pf1N1B4_4891 - FIG00959288: hypothetical protein, at 5,413,753 to 5,414,028 _4891 Pf1N1B4_4892 - Transcriptional regulator, AraC family, at 5,414,306 to 5,415,121 _4892 Pf1N1B4_4893 - O-acetylhomoserine sulfhydrylase (EC 2.5.1.49), at 5,415,173 to 5,416,579 _4893 Pf1N1B4_4894 - Nucleoside-diphosphate-sugar epimerases, at 5,416,611 to 5,416,952 _4894
Group Condition Pf1N1B4_4890 Pf1N1B4_4891 Pf1N1B4_4892 Pf1N1B4_4893 Pf1N1B4_4894
nitrogen source nitrogen source nitrite 0.1mM -0.2 N.D. -0.8 -0.5 -0.8
nitrogen source D-Glucosamine Hydrochloride (N) -1.1 N.D. +0.0 +0.2 -1.0
temperature LB 37C +0.2 N.D. -0.2 -0.2 -1.0
carbon source carbon source Sucrose 0.25mM -0.6 N.D. +0.1 -0.9 +0.3
carbon source Uridine (C) -0.7 N.D. +0.4 -0.6 +0.0
carbon source carbon source Sucrose 0.5mM -0.5 N.D. +0.5 -0.5 -0.1
stress Cisplatin 0.006 mg/ml -0.6 N.D. +0.2 -0.6 +0.5
stress D-Cycloserine 0.1 mg/ml -0.4 N.D. -0.0 -0.6 +0.5
denitrifying Denitrifying in presence of WT Pseudomonas N2-E3; replicate C; timepoint 1 -0.6 N.D. +0.4 -0.9 +0.6
stress Fusidic 0.125 mg/ml -0.5 N.D. +1.0 -1.7 +0.8
nitrogen source Thymine (N) +0.4 N.D. -0.5 +0.2 -0.6
carbon source D-Galacturonic Acid (C) -0.5 N.D. +0.2 -0.6 +0.5
carbon source carbon source Sucrose 1mM +0.1 N.D. +0.4 -1.0 +0.3
stress Thallium(I) acetate 0.008 mg/ml -0.4 N.D. +0.2 -0.5 +0.5
nitrogen source L-Citrulline (N) -0.3 N.D. +0.3 -0.6 +0.5
nitrogen source Cytidine (N) +0.2 N.D. +0.0 +0.7 -0.7
nitrogen source L-Methionine (N) -0.3 N.D. +0.5 -0.2 +0.6
carbon source L-Valine (C) -0.5 N.D. +0.6 -0.3 +0.8
stress Doxycycline hyclate 0.0001 mg/ml -0.2 N.D. +0.9 -0.2 +0.4
carbon source octanoate (C) +0.0 N.D. +0.6 -0.4 +0.8
stress Paraquat dichloride 0.016 mg/mL +0.4 N.D. +0.9 +0.2 -0.2
stress 1-ethyl-3-methylimidazolium chloride 80 mM +0.6 N.D. +0.3 +0.7 -0.2
stress Cholin acetate 20 mM +0.5 N.D. -0.3 +0.6 +0.7
stress methylglyoxal 0.008 vol% +0.6 N.D. +0.8 +0.5 -0.4
stress Sisomicin 0.001 mg/ml +0.3 N.D. +1.0 +0.3 +0.2
denitrifying Nitrate reduction, replicate A t2 +0.6 N.D. +0.5 +0.2 +0.5
stress 1-ethyl-3-methylimidazolium lysine 5 mM +0.4 N.D. +0.4 +0.7 +0.5
stress nitrate 100 mM +0.6 N.D. +0.6 +0.9 +0.1
stress Cholin acetate 10 mM +0.8 N.D. +0.1 +1.0 +0.3
stress Choline Lysine 6 mM +0.5 N.D. +1.0 +1.0 -0.0
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