Fitness for 5 genes in Pseudomonas fluorescens FW300-N1B4

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500 ntPf1N1B4_4860 and Pf1N1B4_4861 are separated by 14 nucleotidesPf1N1B4_4861 and Pf1N1B4_4862 are separated by 257 nucleotidesPf1N1B4_4862 and Pf1N1B4_4863 are separated by 338 nucleotidesPf1N1B4_4863 and Pf1N1B4_4864 overlap by 4 nucleotides Pf1N1B4_4860 - Sensory box sensor histidine kinase/response regulator (EC 2.7.3.-), at 5,373,524 to 5,375,176 _4860 Pf1N1B4_4861 - KaiC, at 5,375,191 to 5,376,693 _4861 Pf1N1B4_4862 - TPR domain protein, at 5,376,951 to 5,378,006 _4862 Pf1N1B4_4863 - Outer membrane protein OprN, at 5,378,345 to 5,379,766 _4863 Pf1N1B4_4864 - Multidrug efflux transporter MexF, at 5,379,763 to 5,382,954 _4864
Group Condition Pf1N1B4_4860 Pf1N1B4_4861 Pf1N1B4_4862 Pf1N1B4_4863 Pf1N1B4_4864
carbon source octanoate (C) -2.0 -0.2 N.D. -0.0 -0.5
nitrogen source Uridine (N) -1.9 -0.7 N.D. -0.3 +0.2
carbon source carbon source Sucrose 1mM -0.1 -1.6 N.D. -0.6 -0.1
carbon source L-Citrulline (C) -1.8 -0.2 N.D. -0.1 -0.2
nophagecontrol TIme6-nophage -1.4 -0.1 N.D. -0.2 -0.0
carbon source L-Leucine (C) -0.8 -0.6 N.D. -0.4 +0.0
denitrifying Denitrifying in presence of WT Pseudomonas N2-E3; replicate C; timepoint 1 -1.5 -0.4 N.D. +0.2 -0.1
carbon source L-Isoleucine (C) -0.6 -1.0 N.D. -0.1 -0.1
anaerobic Nitrate reduction -0.4 -0.4 N.D. -0.2 -0.5
carbon source Cytidine (C) -0.7 -0.7 N.D. -0.2 +0.1
stress Doxycycline hyclate 0.0001 mg/ml -1.4 +0.2 N.D. +0.4 -0.6
carbon source D-Mannose (C) -0.5 -0.3 N.D. -0.7 +0.2
nitrogen source nitrogen source nitrite 0.1mM -1.1 +0.1 N.D. -0.2 -0.0
nitrogen source Thymine (N) -0.9 +0.1 N.D. -0.4 +0.1
carbon source carbon source Sucrose 0.5mM -1.4 -0.4 N.D. +0.6 +0.2
carbon source carbon source Sucrose 1mM -0.5 -0.6 N.D. +0.6 -0.1
nitrogen source Cytidine (N) -0.9 -0.1 N.D. +0.7 -0.2
stress Choline Lysine 6 mM -1.0 -0.2 N.D. +0.6 +0.1
pH Minimal media pH8 -1.0 +0.3 N.D. +0.0 +0.2
stress Sisomicin 0.001 mg/ml -0.6 -0.6 N.D. +0.9 -0.1
nitrogen source nitrogen source nitrite 0.1mM -0.1 -1.0 N.D. +0.7 +0.0
pH Minimal media pH9 -0.7 -0.7 N.D. +0.7 +0.5
stress 5-Hydroxymethylfurfural 2.5 mM +0.0 -0.8 N.D. +0.7 +0.3
pH Minimal media pH9 -0.5 -0.4 N.D. +0.7 +0.5
carbon source carbon source Sucrose 0.25mM +0.7 -0.7 N.D. +0.6 +0.1
carbon source carbon source Sucrose 0.25mM +0.1 -0.3 N.D. +0.9 +0.5
stress nitrate 100 mM -0.3 -0.4 N.D. +1.6 +0.4
stress sodium fluoride 12.5 mM +0.3 +0.7 N.D. +0.7 -0.0
stress copper (II) chloride 2.5 mM +0.9 +0.3 N.D. +0.6 -0.0
temperature LB 37C +1.1 +0.8 N.D. -0.1 -0.0
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