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Fitness for 5 genes in
Pseudomonas fluorescens FW300-N1B4
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500 nt
Pf1N1B4_4717 and Pf1N1B4_4718 are separated by 207 nucleotides
Pf1N1B4_4718 and Pf1N1B4_4719 are separated by 52 nucleotides
Pf1N1B4_4719 and Pf1N1B4_4720 are separated by 146 nucleotides
Pf1N1B4_4720 and Pf1N1B4_4721 are separated by 18 nucleotides
Pf1N1B4_4717 - Agmatinase (EC 3.5.3.11), at 5,213,837 to 5,214,796
_4717
Pf1N1B4_4718 - Transcriptional regulator, DeoR family, at 5,215,004 to 5,215,720
_4718
Pf1N1B4_4719 - Electron transfer flavoprotein, beta subunit, at 5,215,773 to 5,216,114
_4719
Pf1N1B4_4720 - AFG1-like ATPase, at 5,216,261 to 5,217,379
_4720
Pf1N1B4_4721 - Nuclease inhibitor, at 5,217,398 to 5,217,979
_4721
Group
Condition
Pf1N1B4
_4717
Pf1N1B4
_4718
Pf1N1B4
_4719
Pf1N1B4
_4720
Pf1N1B4
_4721
carbon source
carbon source Sucrose 5mM
-1.5
N.D.
N.D.
N.D.
-1.4
carbon source
carbon source Sucrose 5mM
-1.7
N.D.
N.D.
N.D.
-0.3
temperature
LB 37C
-1.1
N.D.
N.D.
N.D.
-0.4
anaerobic
Nitrate reduction
+0.1
N.D.
N.D.
N.D.
-1.6
temperature
LB 37C
-0.1
N.D.
N.D.
N.D.
-1.3
denitrifying
Nitrate reduction, replicate A
-0.2
N.D.
N.D.
N.D.
-1.2
carbon source
carbon source Sucrose 1mM
-1.2
N.D.
N.D.
N.D.
-0.1
anaerobic
Nitrate reduction
+0.4
N.D.
N.D.
N.D.
-1.5
carbon source
L-Arginine (C)
-1.3
N.D.
N.D.
N.D.
+0.2
denitrifying
Nitrate reduction, replicate C t3
-1.8
N.D.
N.D.
N.D.
+0.8
stress
Aluminum chloride 2.5 mM
-1.2
N.D.
N.D.
N.D.
+0.3
carbon source
L-Lactate (C)
-1.2
N.D.
N.D.
N.D.
+0.6
denitrifying
Nitrate reduction, replicate C t2
-1.0
N.D.
N.D.
N.D.
+0.6
anaerobic
N2E2wt with N1B4m denitrifying repB
+0.8
N.D.
N.D.
N.D.
-1.1
carbon source
carbon source Sucrose 0.25mM
+1.1
N.D.
N.D.
N.D.
-1.3
nitrogen source
nitrogen source Ammonium chloride 20mM
+0.7
N.D.
N.D.
N.D.
-0.6
nitrogen source
Cytidine (N)
-0.7
N.D.
N.D.
N.D.
+0.8
nophagecontrol
TIme6-nophage
+0.7
N.D.
N.D.
N.D.
-0.5
carbon source
carbon source Sucrose 1mM
+0.9
N.D.
N.D.
N.D.
-0.4
nitrogen source
nitrogen source nitrate 20mM
+1.3
N.D.
N.D.
N.D.
-0.6
stress
Spectinomycin 0.05 mg/ml
+1.2
N.D.
N.D.
N.D.
-0.3
stress
nitrite 10 mM
+1.0
N.D.
N.D.
N.D.
+0.2
nitrogen source
Thymine (N)
+0.2
N.D.
N.D.
N.D.
+1.0
denitrifying
Denitrifying in presence of WT Pseudomonas N2-E3; replicate C; timepoint 1
+1.4
N.D.
N.D.
N.D.
-0.1
stress
sodium fluoride 12.5 mM
+0.9
N.D.
N.D.
N.D.
+0.6
nitrogen source
nitrate (N)
+0.9
N.D.
N.D.
N.D.
+0.6
nitrogen source
Uridine (N)
+1.0
N.D.
N.D.
N.D.
+0.5
stress
Doxycycline hyclate 0.0001 mg/ml
+0.7
N.D.
N.D.
N.D.
+0.9
nitrogen source
Adenine (N)
+0.7
N.D.
N.D.
N.D.
+1.0
carbon source
octanoate (C)
+0.8
N.D.
N.D.
N.D.
+1.2
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