Fitness for 5 genes in Pseudomonas fluorescens FW300-N1B4

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500 ntPf1N1B4_4716 and Pf1N1B4_4717 are separated by 21 nucleotidesPf1N1B4_4717 and Pf1N1B4_4718 are separated by 207 nucleotidesPf1N1B4_4718 and Pf1N1B4_4719 are separated by 52 nucleotidesPf1N1B4_4719 and Pf1N1B4_4720 are separated by 146 nucleotides Pf1N1B4_4716 - Putrescine ABC transporter putrescine-binding protein PotF (TC 3.A.1.11.2), at 5,212,724 to 5,213,815 _4716 Pf1N1B4_4717 - Agmatinase (EC 3.5.3.11), at 5,213,837 to 5,214,796 _4717 Pf1N1B4_4718 - Transcriptional regulator, DeoR family, at 5,215,004 to 5,215,720 _4718 Pf1N1B4_4719 - Electron transfer flavoprotein, beta subunit, at 5,215,773 to 5,216,114 _4719 Pf1N1B4_4720 - AFG1-like ATPase, at 5,216,261 to 5,217,379 _4720
Group Condition Pf1N1B4_4716 Pf1N1B4_4717 Pf1N1B4_4718 Pf1N1B4_4719 Pf1N1B4_4720
temperature LB 37C -0.9 -1.1 N.D. N.D. N.D.
denitrifying Nitrate reduction, replicate C t3 +0.2 -1.8 N.D. N.D. N.D.
carbon source L-Histidine (C) -1.3 -0.3 N.D. N.D. N.D.
carbon source L-Lactate (C) -0.4 -1.2 N.D. N.D. N.D.
carbon source carbon source Sucrose 5mM +0.1 -1.7 N.D. N.D. N.D.
stress Chloride 300 mM -0.9 -0.5 N.D. N.D. N.D.
anaerobic N2E2wt with N1B4m denitrifying repA -1.3 -0.1 N.D. N.D. N.D.
stress Aluminum chloride 2.5 mM -0.2 -1.2 N.D. N.D. N.D.
carbon source carbon source Sucrose 1mM -0.1 -1.2 N.D. N.D. N.D.
carbon source L-Arginine (C) +0.2 -1.3 N.D. N.D. N.D.
carbon source carbon source Sucrose 5mM +0.5 -1.5 N.D. N.D. N.D.
phagefrs Time6-FRS +0.2 -1.0 N.D. N.D. N.D.
stress Cholin acetate 20 mM -0.9 +0.3 N.D. N.D. N.D.
denitrifying Nitrate reduction, replicate C t2 +0.6 -1.0 N.D. N.D. N.D.
carbon source L-Arabinose (C) -0.7 +0.5 N.D. N.D. N.D.
carbon source D-Glucose (C) -0.7 +0.6 N.D. N.D. N.D.
anaerobic N2E2wt with N1B4m denitrifying repB -0.9 +0.8 N.D. N.D. N.D.
stress sodium fluoride 12.5 mM -0.8 +0.9 N.D. N.D. N.D.
pH Minimal media pH8 -0.4 +0.7 N.D. N.D. N.D.
denitrifying Denitrifying in presence of WT Pseudomonas N2-E3; replicate C; timepoint 1 -1.1 +1.4 N.D. N.D. N.D.
stress nitrite 10 mM -0.2 +1.0 N.D. N.D. N.D.
nitrogen source Cytidine (N) +1.5 -0.7 N.D. N.D. N.D.
stress Spectinomycin 0.05 mg/ml -0.3 +1.2 N.D. N.D. N.D.
nitrogen source nitrogen source nitrate 20mM -0.2 +1.3 N.D. N.D. N.D.
carbon source octanoate (C) +0.5 +0.8 N.D. N.D. N.D.
nitrogen source Ammonium chloride (N) +0.9 +0.6 N.D. N.D. N.D.
stress Paraquat dichloride 0.016 mg/mL +1.4 +0.1 N.D. N.D. N.D.
carbon source carbon source Sucrose 0.25mM +0.5 +1.1 N.D. N.D. N.D.
nitrogen source nitrate (N) +0.8 +0.9 N.D. N.D. N.D.
carbon source carbon source Sucrose 1mM +1.3 +0.9 N.D. N.D. N.D.
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