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Cofit
Protein
Homologs
Fitness for 5 genes in
Pseudomonas fluorescens FW300-N1B4
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500 nt
Pf1N1B4_4688 and Pf1N1B4_4689 are separated by 13 nucleotides
Pf1N1B4_4689 and Pf1N1B4_4690 are separated by 221 nucleotides
Pf1N1B4_4690 and Pf1N1B4_4691 overlap by 1 nucleotides
Pf1N1B4_4691 and Pf1N1B4_4692 are separated by 454 nucleotides
Pf1N1B4_4688 - D-3-phosphoglycerate dehydrogenase (EC 1.1.1.95), at 5,180,534 to 5,181,487
_4688
Pf1N1B4_4689 - hydrolase, alpha/beta fold family, at 5,181,501 to 5,182,544
_4689
Pf1N1B4_4690 - probable membrane protein YPO3302, at 5,182,766 to 5,183,200
_4690
Pf1N1B4_4691 - CopG protein, at 5,183,200 to 5,183,646
_4691
Pf1N1B4_4692 - hypothetical protein, at 5,184,101 to 5,184,631
_4692
Group
Condition
Pf1N1B4
_4688
Pf1N1B4
_4689
Pf1N1B4
_4690
Pf1N1B4
_4691
Pf1N1B4
_4692
nitrogen source
nitrogen source nitrite 0.1mM
-0.5
+0.1
N.D.
-0.8
-0.2
stress
Dimethyl Sulfoxide 6.5 vol%
-0.4
-0.2
N.D.
-0.1
-0.6
temperature
LB 37C
-0.4
+0.1
N.D.
-0.3
-0.8
stress
Fusidic 0.125 mg/ml
+0.5
-0.5
N.D.
-0.9
-0.3
carbon source
butyrate (C)
-0.4
-0.4
N.D.
+0.0
-0.4
stress
methylglyoxal 0.008 vol%
-0.4
-0.2
N.D.
-0.3
-0.2
nitrogen source
L-Isoleucine (N)
+0.1
-0.5
N.D.
-0.1
-0.4
denitrifying
Denitrifying in presence of WT Pseudomonas N2-E3; replicate C; timepoint 1
-0.0
-0.5
N.D.
-0.4
+0.1
lb
LB
-0.4
+0.2
N.D.
-0.2
-0.3
carbon source
m-Inositol (C)
+0.0
-0.4
N.D.
+0.2
-0.5
carbon source
L-Asparagine (C)
-0.0
-0.2
N.D.
+0.2
-0.6
carbon source
carbon source Sucrose 1mM
+0.3
-0.1
N.D.
-0.6
-0.2
carbon source
L-Valine (C)
+0.3
-0.1
N.D.
-0.2
-0.4
stress
Chloride 200 mM
-0.1
+0.2
N.D.
-0.6
+0.1
carbon source
carbon source Sucrose 5mM
+0.2
-0.3
N.D.
+0.2
-0.5
nitrogen source
nitrogen source nitrite 0.5mM
+0.2
+0.3
N.D.
-0.3
-0.4
carbon source
carbon source Sucrose 1mM
-0.2
-0.0
N.D.
+0.4
-0.5
pH
Minimal media pH9
+0.3
+0.2
N.D.
-0.2
-0.5
nitrogen source
D-Glucosamine Hydrochloride (N)
+0.5
-0.2
N.D.
-0.2
-0.2
carbon source
D,L-Lactate (C)
-0.4
-0.1
N.D.
+0.3
+0.2
carbon source
carbon source Sucrose 0.25mM
-0.4
-0.2
N.D.
+0.4
+0.3
stress
Chlorite 1 mM
+0.5
-0.2
N.D.
+0.2
-0.2
carbon source
carbon source Sucrose 5mM
+0.9
-0.2
N.D.
-0.3
-0.0
pH
Minimal media pH9
+0.1
+0.2
N.D.
+0.6
-0.3
stress
Doxycycline hyclate 0.0001 mg/ml
+0.6
-0.3
N.D.
+0.3
+0.1
carbon source
a-Ketoglutaric (C)
+0.2
+0.4
N.D.
-0.1
+0.4
carbon source
D-Fructose (C)
-0.1
+0.1
N.D.
+0.3
+0.6
stress
copper (II) chloride 2.5 mM
-0.0
+0.3
N.D.
+0.0
+0.7
nitrogen source
Cytidine (N)
+0.1
+0.4
N.D.
+0.3
+0.3
stress
nitrite 10 mM
+0.6
+0.2
N.D.
+0.3
+0.3
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