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Protein
Homologs
Fitness for 5 genes in
Pseudomonas fluorescens FW300-N1B4
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500 nt
Pf1N1B4_4641 and Pf1N1B4_4642 are separated by 186 nucleotides
Pf1N1B4_4642 and Pf1N1B4_4643 are separated by 203 nucleotides
Pf1N1B4_4643 and Pf1N1B4_4644 are separated by 370 nucleotides
Pf1N1B4_4644 and Pf1N1B4_4645 are separated by 245 nucleotides
Pf1N1B4_4641 - FIG00954580: hypothetical protein, at 5,118,110 to 5,118,463
_4641
Pf1N1B4_4642 - hypothetical protein, at 5,118,650 to 5,118,763
_4642
Pf1N1B4_4643 - NAD-specific glutamate dehydrogenase (EC 1.4.1.2), large form, at 5,118,967 to 5,123,874
_4643
Pf1N1B4_4644 - hypothetical protein, at 5,124,245 to 5,124,505
_4644
Pf1N1B4_4645 - FIG00954888: hypothetical protein, at 5,124,751 to 5,125,044
_4645
Group
Condition
Pf1N1B4
_4641
Pf1N1B4
_4642
Pf1N1B4
_4643
Pf1N1B4
_4644
Pf1N1B4
_4645
carbon source
L-Arginine (C)
N.D.
N.D.
-2.8
-0.1
N.D.
carbon source
L-Citrulline (C)
N.D.
N.D.
-2.3
-0.3
N.D.
carbon source
octanoate (C)
N.D.
N.D.
-2.4
+0.0
N.D.
anaerobic
N2E2wt with N1B4m denitrifying repA
N.D.
N.D.
+0.0
-2.0
N.D.
carbon source
L-Valine (C)
N.D.
N.D.
-1.3
-0.4
N.D.
carbon source
L-Leucine (C)
N.D.
N.D.
-1.9
+0.2
N.D.
stress
Fusidic 0.125 mg/ml
N.D.
N.D.
-0.2
-1.5
N.D.
stress
Carbenicillin 2 mg/ml
N.D.
N.D.
-0.9
-0.3
N.D.
stress
nitrite 10 mM
N.D.
N.D.
-0.3
-0.9
N.D.
carbon source
carbon source Sucrose 1mM
N.D.
N.D.
+0.0
-1.2
N.D.
carbon source
L-Glutamine (C)
N.D.
N.D.
-0.6
-0.6
N.D.
stress
Rifampicin 0.004 mg/ml
N.D.
N.D.
-1.3
+0.1
N.D.
stress
5-Hydroxymethylfurfural 2.5 mM
N.D.
N.D.
-0.2
-0.9
N.D.
stress
Fusidic 0.0625 mg/ml
N.D.
N.D.
-0.2
-0.8
N.D.
denitrifying
Denitrifying in presence of WT Pseudomonas N2-E3; replicate C; timepoint 1
N.D.
N.D.
-0.1
-0.9
N.D.
stress
Benzethonium chloride 0.0125 mM
N.D.
N.D.
-0.6
-0.4
N.D.
stress
Choline chloride 200 mM
N.D.
N.D.
+0.2
-0.8
N.D.
stress
Sisomicin 0.001 mg/ml
N.D.
N.D.
+0.6
-1.0
N.D.
stress
1-ethyl-3-methylimidazolium lysine 5 mM
N.D.
N.D.
+0.4
-0.7
N.D.
lb
LB
N.D.
N.D.
+0.3
-0.6
N.D.
stress
methylglyoxal 0.004 vol%
N.D.
N.D.
+0.4
-0.6
N.D.
stress
Gentamicin 0.001 mg/ml
N.D.
N.D.
+0.6
-0.7
N.D.
stress
Choline Lysine 6 mM
N.D.
N.D.
+0.6
-0.6
N.D.
stress
methylglyoxal 0.008 vol%
N.D.
N.D.
+0.9
-0.7
N.D.
stress
1-ethyl-3-methylimidazolium chloride 80 mM
N.D.
N.D.
+0.6
-0.3
N.D.
temperature
LB 37C
N.D.
N.D.
+0.8
-0.4
N.D.
pH
Minimal media pH9
N.D.
N.D.
-0.3
+0.9
N.D.
temperature
LB 37C
N.D.
N.D.
+0.9
+0.1
N.D.
anaerobic
Nitrate reduction
N.D.
N.D.
+0.6
+0.4
N.D.
carbon source
carbon source Sucrose 5mM
N.D.
N.D.
+0.3
+1.3
N.D.
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