Fitness for 5 genes in Pseudomonas fluorescens FW300-N1B4

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500 ntPf1N1B4_461 and Pf1N1B4_462 are separated by 231 nucleotidesPf1N1B4_462 and Pf1N1B4_463 overlap by 4 nucleotidesPf1N1B4_463 and Pf1N1B4_464 overlap by 1 nucleotidesPf1N1B4_464 and Pf1N1B4_465 overlap by 4 nucleotides Pf1N1B4_461 - Ribonuclease E (EC 3.1.26.12), at 550,465 to 553,638 _461 Pf1N1B4_462 - CTP:molybdopterin cytidylyltransferase, at 553,870 to 554,463 _462 Pf1N1B4_463 - Xanthine and CO dehydrogenases maturation factor, XdhC/CoxF family, at 554,460 to 555,443 _463 Pf1N1B4_464 - Isoquinoline 1-oxidoreductase beta subunit (EC 1.3.99.16), at 555,443 to 557,764 _464 Pf1N1B4_465 - Isoquinoline 1-oxidoreductase alpha subunit (EC 1.3.99.16), at 557,761 to 558,234 _465
Group Condition Pf1N1B4_461 Pf1N1B4_462 Pf1N1B4_463 Pf1N1B4_464 Pf1N1B4_465
temperature LB 37C N.D. N.D. -0.8 +0.0 -0.6
nitrogen source nitrogen source nitrate 20mM N.D. N.D. -0.3 -0.3 -0.5
carbon source carbon source Sucrose 5mM N.D. N.D. -0.4 +0.1 -0.8
pH Minimal media pH9 N.D. N.D. -0.5 -0.2 -0.3
anaerobic Nitrate reduction N.D. N.D. -0.4 -0.4 -0.1
carbon source carbon source Sucrose 0.25mM N.D. N.D. -0.2 +0.1 -0.8
nitrogen source nitrogen source nitrite 0.5mM N.D. N.D. -0.1 -0.2 -0.5
stress Nalidixic 0.004 mg/ml N.D. N.D. -0.0 -0.1 -0.6
stress methylglyoxal 0.004 vol% N.D. N.D. -0.2 -0.3 -0.3
denitrifying Nitrate reduction, replicate A t1 N.D. N.D. -0.3 -0.1 -0.3
nitrogen source D-Glucosamine Hydrochloride (N) N.D. N.D. -0.4 -0.3 +0.2
denitrifying Nitrate reduction, replicate A t2 N.D. N.D. -0.5 +0.1 -0.1
denitrifying Nitrate reduction, replicate A N.D. N.D. +0.1 -0.2 -0.4
stress D-Cycloserine 0.1 mg/ml N.D. N.D. -0.5 +0.1 +0.1
nitrogen source Cytosine (N) N.D. N.D. +0.4 -0.2 -0.3
nitrogen source Uridine (N) N.D. N.D. +0.4 -0.2 -0.3
denitrifying Denitrifying in presence of WT Pseudomonas N2-E3; replicate B; timepoint 1 N.D. N.D. +0.2 -0.4 +0.1
anaerobic Nitrate reduction N.D. N.D. -0.2 -0.1 +0.4
nitrogen source nitrogen source nitrite 0.1mM N.D. N.D. -0.3 +0.2 +0.2
denitrifying Nitrate reduction, replicate C t3 N.D. N.D. -0.3 -0.0 +0.5
stress Bacitracin 2 mg/ml N.D. N.D. -0.4 +0.2 +0.4
nitrogen source nitrogen source nitrite 0.1mM N.D. N.D. +0.3 +0.4 -0.4
stress Dimethyl Sulfoxide 5 vol% N.D. N.D. -0.2 +0.2 +0.4
carbon source carbon source Sucrose 1mM N.D. N.D. -0.3 -0.4 +1.1
stress Paraquat dichloride 0.016 mg/mL N.D. N.D. +0.4 -0.1 +0.2
carbon source L-Citrulline (C) N.D. N.D. -0.1 +0.1 +0.6
carbon source Cytidine (C) N.D. N.D. -0.0 +0.3 +0.3
carbon source octanoate (C) N.D. N.D. +0.4 +0.3 -0.1
nophagecontrol TIme6-nophage N.D. N.D. +0.2 +0.1 +0.5
temperature LB 37C N.D. N.D. +0.1 +0.3 +0.4
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