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Fitness for 5 genes in
Pseudomonas fluorescens FW300-N1B4
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500 nt
Pf1N1B4_447 and Pf1N1B4_448 are separated by 3 nucleotides
Pf1N1B4_448 and Pf1N1B4_449 overlap by 1 nucleotides
Pf1N1B4_449 and Pf1N1B4_450 are separated by 144 nucleotides
Pf1N1B4_450 and Pf1N1B4_451 are separated by 191 nucleotides
Pf1N1B4_447 - FIG004453: protein YceG like, at 538,996 to 540,210
_447
Pf1N1B4_448 - Aminodeoxychorismate lyase (EC 4.1.3.38), at 540,214 to 541,029
_448
Pf1N1B4_449 - 3-oxoacyl-[acyl-carrier-protein] synthase, KASII (EC 2.3.1.179), at 541,029 to 542,237
_449
Pf1N1B4_450 - Acyl carrier protein, at 542,382 to 542,618
_450
Pf1N1B4_451 - 3-oxoacyl-[acyl-carrier protein] reductase (EC 1.1.1.100), at 542,810 to 543,553
_451
Group
Condition
Pf1N1B4
_447
Pf1N1B4
_448
Pf1N1B4
_449
Pf1N1B4
_450
Pf1N1B4
_451
nophagecontrol
TIme6-nophage
+0.0
-3.4
N.D.
N.D.
N.D.
anaerobic
Nitrate reduction
-0.4
-2.0
N.D.
N.D.
N.D.
stress
Aluminum chloride 2.5 mM
+0.3
-2.7
N.D.
N.D.
N.D.
carbon source
carbon source Sucrose 1mM
+0.7
-3.0
N.D.
N.D.
N.D.
anaerobic
N2E2wt with N1B4m denitrifying repB
-0.3
-1.7
N.D.
N.D.
N.D.
denitrifying
Denitrifying in presence of WT Pseudomonas N2-E3; replicate C; timepoint 1
-0.3
-1.6
N.D.
N.D.
N.D.
stress
Doxycycline hyclate 0.0001 mg/ml
+0.7
-2.7
N.D.
N.D.
N.D.
stress
Carbenicillin 2 mg/ml
-1.8
-0.1
N.D.
N.D.
N.D.
anaerobic
Nitrate reduction
-0.3
-1.5
N.D.
N.D.
N.D.
nitrogen source
nitrogen source nitrate 5mM
+0.1
-1.8
N.D.
N.D.
N.D.
denitrifying
Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate B
-0.3
-1.4
N.D.
N.D.
N.D.
anaerobic
N2E2wt with N1B4m denitrifying repA
-0.4
-1.2
N.D.
N.D.
N.D.
denitrifying
Denitrifying in presence of WT Pseudomonas N2-E3; replicate B; timepoint 1
-0.4
-1.1
N.D.
N.D.
N.D.
carbon source
L-Leucine (C)
+0.2
-1.7
N.D.
N.D.
N.D.
denitrifying
Nitrate reduction in presence of wild-type Pseudomonas N2-E3; replicate A
-0.3
-1.1
N.D.
N.D.
N.D.
nitrogen source
nitrogen source nitrite 0.1mM
-0.1
-1.3
N.D.
N.D.
N.D.
denitrifying
Nitrate reduction, replicate A t2
-0.4
-0.9
N.D.
N.D.
N.D.
denitrifying
Nitrate reduction, replicate C t3
-0.4
-0.8
N.D.
N.D.
N.D.
denitrifying
Nitrate reduction, replicate A
-0.5
-0.7
N.D.
N.D.
N.D.
carbon source
L-Glutamine (C)
+0.0
-1.2
N.D.
N.D.
N.D.
carbon source
carbon source Sucrose 1mM
+0.6
-1.6
N.D.
N.D.
N.D.
carbon source
L-Arabinose (C)
+0.2
-1.1
N.D.
N.D.
N.D.
nitrogen source
L-Methionine (N)
+0.5
-0.9
N.D.
N.D.
N.D.
stress
Cisplatin 0.006 mg/ml
+0.6
-0.8
N.D.
N.D.
N.D.
temperature
LB 37C
+0.1
+1.1
N.D.
N.D.
N.D.
nitrogen source
Cytidine (N)
+0.7
+0.5
N.D.
N.D.
N.D.
stress
Chloride 300 mM
+0.4
+1.0
N.D.
N.D.
N.D.
stress
Fusidic 0.0625 mg/ml
+0.9
+0.6
N.D.
N.D.
N.D.
stress
Choline chloride 200 mM
+1.3
+0.3
N.D.
N.D.
N.D.
stress
nitrate 100 mM
+0.9
+0.7
N.D.
N.D.
N.D.
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