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Fitness for 5 genes in
Pseudomonas fluorescens FW300-N1B4
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500 nt
Pf1N1B4_4449 and Pf1N1B4_4450 are separated by 179 nucleotides
Pf1N1B4_4450 and Pf1N1B4_4451 are separated by 133 nucleotides
Pf1N1B4_4451 and Pf1N1B4_4452 are separated by 795 nucleotides
Pf1N1B4_4452 and Pf1N1B4_4453 are separated by 428 nucleotides
Pf1N1B4_4449 - Permeases of the major facilitator superfamily, at 4,908,027 to 4,909,649
_4449
Pf1N1B4_4450 - Choline dehydrogenase (EC 1.1.99.1), at 4,909,829 to 4,911,478
_4450
Pf1N1B4_4451 - Transcriptional regulator, LysR family, at 4,911,612 to 4,912,496
_4451
Pf1N1B4_4452 - hypothetical protein, at 4,913,292 to 4,913,438
_4452
Pf1N1B4_4453 - Sensory histidine kinase QseC, at 4,913,867 to 4,915,270
_4453
Group
Condition
Pf1N1B4
_4449
Pf1N1B4
_4450
Pf1N1B4
_4451
Pf1N1B4
_4452
Pf1N1B4
_4453
nitrogen source
nitrogen source nitrate 20mM
-0.3
-0.0
-0.6
N.D.
-0.4
nitrogen source
Uridine (N)
-0.1
-1.5
-0.0
N.D.
+0.3
nitrogen source
L-Methionine (N)
-1.1
+0.1
-0.0
N.D.
-0.2
carbon source
carbon source Sucrose 5mM
+0.1
-0.4
-0.3
N.D.
-0.5
carbon source
L-Valine (C)
-0.8
-0.6
-0.3
N.D.
+0.5
nitrogen source
D-Glucosamine Hydrochloride (N)
+0.0
-1.3
-0.1
N.D.
+0.2
phagefrs
Time16-FRS
-0.4
-0.7
+0.5
N.D.
-0.4
carbon source
carbon source Sucrose 20mM
+0.1
-0.5
-0.1
N.D.
-0.6
carbon source
carbon source Sucrose 0.25mM
-0.3
-0.9
-0.2
N.D.
+0.5
anaerobic
N2E2wt with N1B4m denitrifying repA
-0.6
+0.2
+0.3
N.D.
-0.8
stress
Benzethonium chloride 0.0125 mM
-1.0
+0.5
-0.3
N.D.
-0.1
nitrogen source
nitrate (N)
-0.1
-0.8
+0.3
N.D.
-0.2
denitrifying
Denitrifying in presence of WT Pseudomonas N2-E3; replicate C; timepoint 1
-0.5
-0.4
+0.9
N.D.
-0.7
carbon source
acetate (C)
-0.4
+0.5
+0.0
N.D.
-0.4
carbon source
L-Glutamic (C)
-0.5
-0.3
+0.1
N.D.
+0.5
stress
Cephalothin 2.5 mg/ml
-0.2
-0.6
+0.1
N.D.
+0.5
pH
Minimal media pH9
-0.7
-0.1
+0.4
N.D.
+0.3
stress
Sisomicin 0.001 mg/ml
+0.5
-0.7
-0.1
N.D.
+0.2
carbon source
m-Inositol (C)
-0.8
+0.4
+0.1
N.D.
+0.3
nitrogen source
Cytosine (N)
-0.2
-0.6
+0.7
N.D.
+0.2
nitrogen source
Ammonium chloride (N)
-0.2
-0.4
+0.2
N.D.
+0.6
stress
Chloride 200 mM
+0.5
-0.5
+0.3
N.D.
-0.1
carbon source
L-Alanine (C)
-0.5
+0.0
+0.3
N.D.
+0.5
stress
Chlorite 1 mM
+0.3
-0.6
+0.3
N.D.
+0.4
stress
Choline Lysine 6 mM
+0.7
-0.3
-0.2
N.D.
+0.4
carbon source
D-Glucose (C)
-0.3
+0.5
+0.3
N.D.
+0.2
carbon source
Uridine (C)
-0.2
-0.1
+0.5
N.D.
+0.5
carbon source
octanoate (C)
-0.7
+0.1
+0.6
N.D.
+0.7
phagefrs
Time6-FRS
+0.4
+0.2
+0.3
N.D.
+0.4
stress
Fusidic 0.125 mg/ml
+0.5
+0.1
+0.1
N.D.
+0.8
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