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Cofit
Protein
Homologs
Fitness for 5 genes in
Pseudomonas fluorescens FW300-N1B4
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Top 30 experiments (either direction), sorted by average fitness
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500 nt
Pf1N1B4_4317 and Pf1N1B4_4318 are separated by 147 nucleotides
Pf1N1B4_4318 and Pf1N1B4_4319 are separated by 576 nucleotides
Pf1N1B4_4319 and Pf1N1B4_4320 overlap by 4 nucleotides
Pf1N1B4_4320 and Pf1N1B4_4321 overlap by 7 nucleotides
Pf1N1B4_4317 - DNA/RNA non-specific endonuclease, at 4,778,421 to 4,779,251
_4317
Pf1N1B4_4318 - hypothetical protein, at 4,779,399 to 4,780,760
_4318
Pf1N1B4_4319 - Phosphonate ABC transporter permease protein phnE1 (TC 3.A.1.9.1), at 4,781,337 to 4,782,104
_4319
Pf1N1B4_4320 - Phosphonate ABC transporter permease protein phnE2 (TC 3.A.1.9.1), at 4,782,101 to 4,782,928
_4320
Pf1N1B4_4321 - Phosphonate ABC transporter ATP-binding protein (TC 3.A.1.9.1), at 4,782,922 to 4,783,719
_4321
Group
Condition
Pf1N1B4
_4317
Pf1N1B4
_4318
Pf1N1B4
_4319
Pf1N1B4
_4320
Pf1N1B4
_4321
carbon source
carbon source Sucrose 1mM
+0.2
-1.1
+0.3
-2.6
N.D.
anaerobic
Nitrate reduction
-1.9
-1.1
-0.1
+0.2
N.D.
carbon source
Carnitine Hydrochloride (C)
-0.6
-1.3
+0.2
-1.3
N.D.
phagefrs
Time6-FRS
-0.9
-1.0
-0.0
-0.7
N.D.
anaerobic
Nitrate reduction
-1.9
-1.9
+0.9
+0.5
N.D.
stress
Cephalothin 2.5 mg/ml
-1.4
-0.2
-0.1
-0.8
N.D.
stress
Spectinomycin 0.05 mg/ml
+0.1
-1.1
-0.6
-0.8
N.D.
stress
Rifampicin 0.004 mg/ml
-0.6
-1.4
+0.2
-0.4
N.D.
carbon source
L-Alanine (C)
-0.6
-0.7
+0.2
-0.8
N.D.
carbon source
carbon source Sucrose 5mM
-0.1
+0.8
+0.3
-2.9
N.D.
anaerobic
N2E2wt with N1B4m denitrifying repA
-0.6
-0.9
-0.7
+0.4
N.D.
stress
sodium fluoride 12.5 mM
-1.1
-0.5
+0.3
-0.3
N.D.
carbon source
carbon source Sucrose 1mM
-2.1
+0.8
-0.1
-0.2
N.D.
carbon source
L-Asparagine (C)
+0.3
-1.5
+0.1
-0.5
N.D.
anaerobic
Nitrate reduction
-1.3
-0.8
+0.2
+0.3
N.D.
stress
Dimethyl Sulfoxide 6.5 vol%
+0.4
-0.5
+0.2
-1.7
N.D.
temperature
LB 37C
-1.0
+1.3
-1.3
-0.5
N.D.
carbon source
octanoate (C)
+0.5
-0.8
+0.2
-1.1
N.D.
nitrogen source
L-Methionine (N)
+0.3
-0.8
-1.0
+0.3
N.D.
carbon source
D-Fructose (C)
-1.1
+0.4
+0.2
-0.6
N.D.
denitrifying
Denitrifying in presence of WT Pseudomonas N2-E3; replicate C; timepoint 1
-2.1
-0.1
+0.3
+0.8
N.D.
carbon source
L-Citrulline (C)
+0.6
-0.8
+0.1
-0.7
N.D.
denitrifying
Nitrate reduction, replicate C
-0.7
-0.6
-0.3
+0.7
N.D.
nitrogen source
nitrogen source nitrite 0.1mM
-0.7
+0.2
-0.8
+0.8
N.D.
carbon source
carbon source Sucrose 0.5mM
+0.0
-0.7
-0.4
+1.4
N.D.
temperature
LB 37C
-0.8
-0.0
+0.7
+0.8
N.D.
nitrogen source
Uridine (N)
+1.2
-0.6
+0.4
-0.3
N.D.
pH
Minimal media pH9
+0.6
-0.8
+0.6
+1.0
N.D.
nitrogen source
nitrate (N)
+1.7
+0.3
+0.3
-0.2
N.D.
carbon source
carbon source Sucrose 0.25mM
+0.3
+0.7
+0.5
+0.7
N.D.
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