Fitness for 5 genes in Pseudomonas fluorescens FW300-N1B4

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500 ntPf1N1B4_4266 and Pf1N1B4_4267 overlap by 23 nucleotidesPf1N1B4_4267 and Pf1N1B4_4268 are separated by 269 nucleotidesPf1N1B4_4268 and Pf1N1B4_4269 are separated by 341 nucleotidesPf1N1B4_4269 and Pf1N1B4_4270 overlap by 4 nucleotides Pf1N1B4_4266 - sensor histidine kinase, at 4,729,669 to 4,731,063 _4266 Pf1N1B4_4267 - DNA-binding response regulator, at 4,731,041 to 4,731,724 _4267 Pf1N1B4_4268 - Mll5186 protein, at 4,731,994 to 4,732,944 _4268 Pf1N1B4_4269 - Hydrogen cyanide synthase HcnA / Opine oxidase subunit C, at 4,733,286 to 4,733,648 _4269 Pf1N1B4_4270 - Hydrogen cyanide synthase HcnB / Opine oxidase subunit A, at 4,733,645 to 4,735,054 _4270
Group Condition Pf1N1B4_4266 Pf1N1B4_4267 Pf1N1B4_4268 Pf1N1B4_4269 Pf1N1B4_4270
denitrifying Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate B N.D. N.D. -0.4 N.D. -0.4
temperature LB 37C N.D. N.D. -0.7 N.D. -0.0
nitrogen source L-Valine (N) N.D. N.D. -0.5 N.D. -0.2
stress Fusidic 0.125 mg/ml N.D. N.D. -0.1 N.D. -0.5
carbon source carbon source Sucrose 5mM N.D. N.D. -0.0 N.D. -0.6
stress Sisomicin 0.001 mg/ml N.D. N.D. -0.4 N.D. -0.2
temperature LB 10C N.D. N.D. -0.4 N.D. -0.2
carbon source carbon source Sucrose 1mM N.D. N.D. -0.0 N.D. -0.6
denitrifying Denitrifying in presence of WT Pseudomonas N2-E3; replicate C; timepoint 1 N.D. N.D. +0.2 N.D. -0.7
nitrogen source Cytosine (N) N.D. N.D. -0.6 N.D. +0.0
stress Gentamicin 0.001 mg/ml N.D. N.D. -0.6 N.D. +0.1
denitrifying Nitrate reduction, replicate B N.D. N.D. +0.5 N.D. -0.9
carbon source octanoate (C) N.D. N.D. -0.5 N.D. +0.2
stress Spectinomycin 0.025 mg/ml N.D. N.D. +0.1 N.D. -0.4
carbon source carbon source Sucrose 20mM N.D. N.D. +0.3 N.D. -0.6
nitrogen source nitrogen source nitrate 5mM N.D. N.D. +0.3 N.D. -0.5
nitrogen source nitrogen source nitrite 0.1mM N.D. N.D. -0.6 N.D. +0.5
stress Doxycycline hyclate 0.0001 mg/ml N.D. N.D. +0.6 N.D. -0.7
nitrogen source Cytidine (N) N.D. N.D. -0.3 N.D. +0.3
stress Nalidixic 0.004 mg/ml N.D. N.D. -0.4 N.D. +0.3
carbon source carbon source Sucrose 0.25mM N.D. N.D. -0.4 N.D. +0.3
pH Minimal media pH9 N.D. N.D. -0.4 N.D. +0.4
denitrifying Nitrate reduction, replicate A N.D. N.D. +0.4 N.D. -0.3
carbon source D-Ribose (C) N.D. N.D. +0.4 N.D. -0.2
carbon source L-Valine (C) N.D. N.D. -0.2 N.D. +0.5
pH Minimal media pH9 N.D. N.D. -0.4 N.D. +0.8
carbon source D,L-Lactate (C) N.D. N.D. +0.4 N.D. +0.2
stress Carbenicillin 2 mg/ml N.D. N.D. +0.4 N.D. +0.3
carbon source carbon source Sucrose 1mM N.D. N.D. +0.5 N.D. +0.3
carbon source carbon source Sucrose 0.5mM N.D. N.D. +0.7 N.D. +0.5
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