Fitness for 5 genes in Pseudomonas fluorescens FW300-N1B4

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500 ntPf1N1B4_4256 and Pf1N1B4_4257 are separated by 224 nucleotidesPf1N1B4_4257 and Pf1N1B4_4258 are separated by 65 nucleotidesPf1N1B4_4258 and Pf1N1B4_4259 are separated by 160 nucleotidesPf1N1B4_4259 and Pf1N1B4_4260 are separated by 37 nucleotides Pf1N1B4_4256 - Probable transmembrane protein, at 4,718,468 to 4,719,148 _4256 Pf1N1B4_4257 - L-serine dehydratase, (PLP)-dependent (EC 4.3.1.17) @ Threonine dehydratase biosynthetic (EC 4.3.1.19), at 4,719,373 to 4,720,305 _4257 Pf1N1B4_4258 - Ribonucleotide reductase, alpha subunit, at 4,720,371 to 4,720,610 _4258 Pf1N1B4_4259 - hypothetical protein, at 4,720,771 to 4,721,544 _4259 Pf1N1B4_4260 - FIG00965772: hypothetical protein, at 4,721,582 to 4,721,935 _4260
Group Condition Pf1N1B4_4256 Pf1N1B4_4257 Pf1N1B4_4258 Pf1N1B4_4259 Pf1N1B4_4260
nitrogen source Adenine (N) -1.4 -1.2 N.D. -0.4 -0.3
temperature LB 37C -0.8 -0.9 N.D. +0.3 -1.4
carbon source carbon source Sucrose 5mM -0.6 -0.5 N.D. -1.6 +0.2
nitrogen source Thymine (N) -0.9 -1.0 N.D. -0.5 +0.2
anaerobic Nitrate reduction -0.7 -0.7 N.D. -0.1 -0.7
nitrogen source Cytosine (N) -0.5 -0.7 N.D. -0.7 -0.2
carbon source carbon source Sucrose 0.5mM -1.9 +1.2 N.D. -0.2 -1.2
carbon source L-Arginine (C) +0.1 -1.2 N.D. +0.1 -0.9
stress Chloride 300 mM -0.3 -1.6 N.D. +0.0 +0.0
nitrogen source nitrogen source nitrite 0.5mM +0.1 -1.0 N.D. -0.7 +0.2
carbon source octanoate (C) +0.5 -1.1 N.D. -0.6 -0.2
pH Minimal media pH9 +0.1 -1.7 N.D. +0.3 -0.2
nitrogen source L-Methionine (N) -0.4 -0.5 N.D. +0.4 -0.9
carbon source carbon source Sucrose 0.25mM -0.6 +0.5 N.D. +0.3 -1.3
carbon source pyruvate (C) +0.5 -1.2 N.D. -0.7 +0.4
anaerobic N2E2wt with N1B4m denitrifying repA -0.3 -0.9 N.D. +0.5 -0.3
carbon source Fumarate (C) +0.4 -0.9 N.D. -0.5 +0.3
stress nitrite 10 mM -1.0 -0.4 N.D. +0.5 +0.3
stress Choline Lysine 6 mM -1.3 +0.7 N.D. +0.2 -0.0
stress Fusidic 0.0625 mg/ml +0.4 -0.4 N.D. +0.4 -0.8
carbon source D-Glucose (C) +0.4 -0.8 N.D. -0.3 +0.4
nitrogen source L-Isoleucine (N) +0.8 -1.2 N.D. +0.4 -0.3
nitrogen source L-Leucine (N) +0.4 -0.8 N.D. +0.4 -0.4
phagefrs Time16-FRS -0.8 +0.8 N.D. -0.3 +0.2
carbon source D,L-Lactate (C) +0.9 -0.8 N.D. -0.2 +0.1
carbon source L-Alanine (C) +0.8 -0.5 N.D. +0.3 -0.4
denitrifying Denitrifying in presence of WT Pseudomonas N2-E3; replicate C; timepoint 1 -0.8 +0.6 N.D. +0.1 +0.6
stress sodium fluoride 12.5 mM +0.3 +0.4 N.D. -0.7 +0.6
phagefrs Time6-FRS +0.1 +1.3 N.D. -0.6 +0.2
stress Fusidic 0.125 mg/ml +1.2 -0.4 N.D. +0.6 -0.1
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