Fitness for 5 genes in Pseudomonas fluorescens FW300-N1B4

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500 ntPf1N1B4_417 and Pf1N1B4_418 are separated by 216 nucleotidesPf1N1B4_418 and Pf1N1B4_419 are separated by 139 nucleotidesPf1N1B4_419 and Pf1N1B4_420 are separated by 394 nucleotidesPf1N1B4_420 and Pf1N1B4_421 are separated by 14 nucleotides Pf1N1B4_417 - lipoprotein, putative, at 486,106 to 486,711 _417 Pf1N1B4_418 - Molybdenum cofactor biosynthesis protein MoaA, at 486,928 to 487,896 _418 Pf1N1B4_419 - Transcriptional regulator, TetR family, at 488,036 to 488,674 _419 Pf1N1B4_420 - Putative deoxyribonuclease YcfH, at 489,069 to 489,860 _420 Pf1N1B4_421 - Type IV pilus biogenesis protein PilZ, at 489,875 to 490,231 _421
Group Condition Pf1N1B4_417 Pf1N1B4_418 Pf1N1B4_419 Pf1N1B4_420 Pf1N1B4_421
stress Benzethonium chloride 0.0125 mM -0.1 -0.4 -2.7 -0.8 -1.0
carbon source carbon source Sucrose 1mM -0.1 -0.6 -3.5 -0.3 -0.2
carbon source carbon source Sucrose 0.25mM +0.0 -0.2 -3.7 -0.1 -0.0
nitrogen source nitrogen source nitrate 20mM -0.2 -2.7 -0.5 -0.0 -0.4
carbon source carbon source Sucrose 1mM +0.2 -0.2 -3.7 -0.1 -0.0
anaerobic N2E2wt with N1B4m denitrifying repA +0.2 -2.5 -1.1 -0.2 +0.2
nitrogen source nitrogen source nitrate 20mM -0.3 -2.4 -0.6 +0.1 -0.1
nitrogen source nitrogen source Ammonium chloride 20mM +0.2 -0.5 -2.9 +0.2 -0.3
denitrifying Nitrate reduction, replicate C +0.2 -0.9 -1.5 -0.1 -0.9
nitrogen source nitrate (N) +0.2 -2.7 -0.7 -0.1 +0.0
denitrifying Nitrate reduction, replicate A +0.3 -0.3 -2.0 +0.1 -1.2
carbon source Carnitine Hydrochloride (C) +0.1 -1.4 -1.6 -0.1 +0.1
carbon source carbon source Sucrose 0.5mM -0.1 +0.1 -2.7 -0.2 +0.1
nitrogen source nitrogen source nitrite 0.1mM -0.2 -0.6 -1.3 -0.1 -0.6
denitrifying Nitrate reduction, replicate B +0.5 -0.1 -1.7 -0.4 -0.7
nitrogen source nitrogen source nitrate 5mM -0.1 -2.5 +0.2 -0.1 +0.0
carbon source L-Histidine (C) -0.0 -1.1 -0.9 -0.2 -0.2
nitrogen source nitrogen source nitrate 5mM +0.6 -2.6 -0.3 +0.1 -0.1
pH Minimal media pH7 -0.1 -0.3 -1.7 -0.1 -0.2
anaerobic N2E2wt with N1B4m denitrifying repA +0.1 -2.2 +0.0 -0.2 +0.1
temperature LB 37C -0.8 -0.5 -1.1 +0.3 +0.3
stress Sisomicin 0.001 mg/ml +0.1 +0.1 -2.1 +0.1 +0.0
stress Doxycycline hyclate 0.0001 mg/ml -0.2 +0.1 -1.6 +0.4 -0.4
stress Fusidic 0.125 mg/ml +0.1 +0.4 -0.8 -0.7 -0.7
nitrogen source D-Glucosamine Hydrochloride (N) +0.1 -0.1 -1.7 +0.4 -0.3
stress Fusidic 0.0625 mg/ml -0.3 +0.4 -1.6 +0.1 -0.1
nitrogen source Cytidine (N) +0.2 +0.2 -1.8 -0.2 +0.2
denitrifying Denitrifying in presence of WT Pseudomonas N2-E3; replicate B; timepoint 1 +0.5 -0.4 -1.2 +0.1 -0.4
nitrogen source Uridine (N) +0.7 -0.1 -1.4 -0.4 +0.1
carbon source carbon source Sucrose 0.25mM +0.7 -0.2 +0.9 -0.4 +1.1
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