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Protein
Homologs
Fitness for 5 genes in
Pseudomonas fluorescens FW300-N1B4
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500 nt
Pf1N1B4_4144 and Pf1N1B4_4145 are separated by 1483 nucleotides
Pf1N1B4_4145 and Pf1N1B4_4146 are separated by 27 nucleotides
Pf1N1B4_4146 and Pf1N1B4_4147 are separated by 1209 nucleotides
Pf1N1B4_4147 and Pf1N1B4_4148 are separated by 150 nucleotides
Pf1N1B4_4144 - Mobile element protein, at 4,589,228 to 4,589,341
_4144
Pf1N1B4_4145 - hypothetical protein, at 4,590,825 to 4,590,992
_4145
Pf1N1B4_4146 - Transposase, at 4,591,020 to 4,591,148
_4146
Pf1N1B4_4147 - hypothetical protein, at 4,592,358 to 4,592,471
_4147
Pf1N1B4_4148 - hypothetical protein, at 4,592,622 to 4,592,738
_4148
Group
Condition
Pf1N1B4
_4144
Pf1N1B4
_4145
Pf1N1B4
_4146
Pf1N1B4
_4147
Pf1N1B4
_4148
anaerobic
Nitrate reduction
N.D.
-2.0
N.D.
N.D.
N.D.
temperature
LB 37C
N.D.
-1.3
N.D.
N.D.
N.D.
carbon source
carbon source Sucrose 20mM
N.D.
-1.1
N.D.
N.D.
N.D.
stress
Spectinomycin 0.05 mg/ml
N.D.
-1.0
N.D.
N.D.
N.D.
nitrogen source
nitrogen source nitrate 20mM
N.D.
-0.9
N.D.
N.D.
N.D.
denitrifying
Nitrate reduction, replicate B
N.D.
-0.9
N.D.
N.D.
N.D.
anaerobic
N2E2wt with N1B4m denitrifying repA
N.D.
-0.9
N.D.
N.D.
N.D.
carbon source
D-Glucose (C)
N.D.
-0.8
N.D.
N.D.
N.D.
stress
Bacitracin 2 mg/ml
N.D.
-0.8
N.D.
N.D.
N.D.
carbon source
Fumarate (C)
N.D.
-0.7
N.D.
N.D.
N.D.
carbon source
a-Ketoglutaric (C)
N.D.
-0.7
N.D.
N.D.
N.D.
carbon source
acetate (C)
N.D.
-0.7
N.D.
N.D.
N.D.
nitrogen source
Thymine (N)
N.D.
+0.7
N.D.
N.D.
N.D.
nitrogen source
Ammonium chloride (N)
N.D.
+0.7
N.D.
N.D.
N.D.
carbon source
Putrescine (C)
N.D.
+0.7
N.D.
N.D.
N.D.
nitrogen source
nitrogen source Ammonium chloride 20mM
N.D.
+0.7
N.D.
N.D.
N.D.
carbon source
L-Alanine (C)
N.D.
+0.7
N.D.
N.D.
N.D.
carbon source
L-Asparagine (C)
N.D.
+0.8
N.D.
N.D.
N.D.
nitrogen source
L-Isoleucine (N)
N.D.
+0.8
N.D.
N.D.
N.D.
stress
Cholin acetate 10 mM
N.D.
+0.8
N.D.
N.D.
N.D.
denitrifying
Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate A
N.D.
+0.8
N.D.
N.D.
N.D.
nitrogen source
Putrescine (N)
N.D.
+0.8
N.D.
N.D.
N.D.
stress
Chloride 200 mM
N.D.
+0.9
N.D.
N.D.
N.D.
nitrogen source
L-Citrulline (N)
N.D.
+0.9
N.D.
N.D.
N.D.
carbon source
D-Galacturonic Acid (C)
N.D.
+0.9
N.D.
N.D.
N.D.
denitrifying
Nitrate reduction, replicate A t1
N.D.
+0.9
N.D.
N.D.
N.D.
carbon source
D-Trehalose (C)
N.D.
+1.0
N.D.
N.D.
N.D.
denitrifying
Nitrate reduction, replicate A t2
N.D.
+1.1
N.D.
N.D.
N.D.
denitrifying
Nitrate reduction, replicate C t1
N.D.
+1.7
N.D.
N.D.
N.D.
nitrogen source
Adenine (N)
N.D.
+1.7
N.D.
N.D.
N.D.
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