Fitness for 5 genes in Pseudomonas fluorescens FW300-N1B4

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500 ntPf1N1B4_4119 and Pf1N1B4_4120 are separated by 67 nucleotidesPf1N1B4_4120 and Pf1N1B4_4121 are separated by 101 nucleotidesPf1N1B4_4121 and Pf1N1B4_4122 are separated by 270 nucleotidesPf1N1B4_4122 and Pf1N1B4_4123 are separated by 48 nucleotides Pf1N1B4_4119 - dioxygenase, at 4,562,080 to 4,562,391 _4119 Pf1N1B4_4120 - hypothetical protein, at 4,562,459 to 4,562,647 _4120 Pf1N1B4_4121 - putative lipoprotein, at 4,562,749 to 4,563,291 _4121 Pf1N1B4_4122 - Acetyltransferase, at 4,563,562 to 4,564,062 _4122 Pf1N1B4_4123 - Isochorismate pyruvate-lyase (EC 4.-.-.-), at 4,564,111 to 4,564,431 _4123
Group Condition Pf1N1B4_4119 Pf1N1B4_4120 Pf1N1B4_4121 Pf1N1B4_4122 Pf1N1B4_4123
temperature LB 37C -1.8 N.D. +0.0 -0.7 -0.6
anaerobic N2E2wt with N1B4m denitrifying repB +0.4 N.D. +0.1 +0.1 -3.0
nophagecontrol TIme6-nophage -0.4 N.D. +0.0 -0.4 -1.4
stress Cisplatin 0.006 mg/ml -0.3 N.D. +0.1 -0.2 -1.6
stress Fusidic 0.125 mg/ml -1.2 N.D. -0.1 -0.0 -0.6
stress Cholin acetate 20 mM -0.2 N.D. +0.1 +0.1 -1.8
stress copper (II) chloride 2.5 mM -0.9 N.D. +0.1 -0.3 -0.6
carbon source a-Ketoglutaric (C) +0.3 N.D. -0.4 -0.4 -1.1
denitrifying Nitrate reduction, replicate C -1.0 N.D. -0.4 +0.4 -0.6
carbon source L-Glutamic (C) -0.5 N.D. -0.1 +0.4 -1.4
stress Carbenicillin 2 mg/ml +0.0 N.D. +0.2 +0.3 -1.8
carbon source L-Histidine (C) +0.3 N.D. -0.1 -0.1 -1.5
denitrifying Nitrate reduction, replicate A -0.4 N.D. +0.0 +0.2 -1.2
stress Doxycycline hyclate 0.0001 mg/ml -1.5 N.D. +0.5 +0.4 -0.5
carbon source carbon source Sucrose 1mM -1.2 N.D. +0.5 -0.7 +0.4
carbon source octanoate (C) -0.7 N.D. +0.3 +0.4 -0.6
carbon source carbon source Sucrose 0.5mM -1.0 N.D. +0.3 -0.2 +0.4
stress Bacitracin 2 mg/ml -1.1 N.D. +0.2 -0.3 +0.9
temperature LB 37C -1.7 N.D. +0.3 +0.3 +0.9
nitrogen source D-Glucosamine Hydrochloride (N) -0.5 N.D. -0.4 +0.8 -0.2
nitrogen source Carnitine Hydrochloride (N) -0.9 N.D. -0.1 +0.1 +0.7
carbon source carbon source Sucrose 1mM -1.0 N.D. -0.0 +0.1 +1.0
carbon source carbon source Sucrose 5mM -0.9 N.D. -0.0 -0.2 +1.3
stress Benzethonium chloride 0.0125 mM -0.9 N.D. +0.3 -0.1 +0.8
nitrogen source nitrogen source nitrite 0.1mM -0.9 N.D. +0.5 -0.3 +0.9
nitrogen source Uridine (N) +0.8 N.D. +0.4 -0.1 -0.6
stress Rifampicin 0.004 mg/ml -0.6 N.D. +0.2 -0.2 +1.0
carbon source carbon source Sucrose 5mM -0.7 N.D. +0.4 +0.0 +1.0
stress benzoic 0.58 mg/ml +0.4 N.D. +0.1 -0.3 +1.1
denitrifying Denitrifying in presence of WT Pseudomonas N2-E3; replicate C; timepoint 1 +0.7 N.D. -0.2 +0.1 +1.2
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