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Cofit
Protein
Homologs
Fitness for 5 genes in
Pseudomonas fluorescens FW300-N1B4
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500 nt
Pf1N1B4_4091 and Pf1N1B4_4092 are separated by 196 nucleotides
Pf1N1B4_4092 and Pf1N1B4_4093 are separated by 159 nucleotides
Pf1N1B4_4093 and Pf1N1B4_4094 are separated by 225 nucleotides
Pf1N1B4_4094 and Pf1N1B4_4095 are separated by 10 nucleotides
Pf1N1B4_4091 - Probable signal peptide protein, at 4,539,825 to 4,540,565
_4091
Pf1N1B4_4092 - Cytosine/purine/uracil/thiamine/allantoin permease family protein, at 4,540,762 to 4,542,174
_4092
Pf1N1B4_4093 - Alkyl hydroperoxide reductase subunit C-like protein, at 4,542,334 to 4,542,987
_4093
Pf1N1B4_4094 - Uncharacterized conserved protein, at 4,543,213 to 4,543,710
_4094
Pf1N1B4_4095 - Transcriptional regulator, LacI family, at 4,543,721 to 4,544,722
_4095
Group
Condition
Pf1N1B4
_4091
Pf1N1B4
_4092
Pf1N1B4
_4093
Pf1N1B4
_4094
Pf1N1B4
_4095
carbon source
carbon source Sucrose 0.5mM
-1.2
-0.5
N.D.
N.D.
N.D.
carbon source
carbon source Sucrose 0.25mM
-0.9
-0.7
N.D.
N.D.
N.D.
temperature
LB 37C
-0.8
-0.5
N.D.
N.D.
N.D.
temperature
LB 37C
-1.4
+0.1
N.D.
N.D.
N.D.
carbon source
carbon source Sucrose 0.25mM
-0.6
-0.5
N.D.
N.D.
N.D.
stress
Rifampicin 0.004 mg/ml
-0.3
-0.4
N.D.
N.D.
N.D.
anaerobic
N2E2wt with N1B4m denitrifying repA
-0.7
+0.0
N.D.
N.D.
N.D.
nitrogen source
nitrate (N)
+0.3
-0.4
N.D.
N.D.
N.D.
denitrifying
Denitrifying in presence of WT Pseudomonas N2-E3; replicate C; timepoint 1
-0.6
+0.5
N.D.
N.D.
N.D.
stress
Gentamicin 0.001 mg/ml
+0.3
-0.3
N.D.
N.D.
N.D.
carbon source
carbon source Sucrose 5mM
+0.4
-0.3
N.D.
N.D.
N.D.
phagefrs
Time16-FRS
+0.4
-0.2
N.D.
N.D.
N.D.
nitrogen source
L-Leucine (N)
+0.8
-0.2
N.D.
N.D.
N.D.
carbon source
L-Glutamic (C)
+0.3
+0.4
N.D.
N.D.
N.D.
carbon source
L-Citrulline (C)
+0.4
+0.2
N.D.
N.D.
N.D.
carbon source
Cytidine (C)
+0.3
+0.3
N.D.
N.D.
N.D.
denitrifying
Nitrate reduction, replicate C t2
+0.2
+0.4
N.D.
N.D.
N.D.
carbon source
Fumarate (C)
+0.4
+0.3
N.D.
N.D.
N.D.
nitrogen source
L-Isoleucine (N)
+0.5
+0.2
N.D.
N.D.
N.D.
nitrogen source
L-Valine (N)
+0.5
+0.2
N.D.
N.D.
N.D.
carbon source
L-Leucine (C)
+0.3
+0.5
N.D.
N.D.
N.D.
carbon source
casaminos (C)
+0.7
+0.1
N.D.
N.D.
N.D.
pH
Minimal media pH9
+0.5
+0.3
N.D.
N.D.
N.D.
nitrogen source
D-Alanine (N)
+0.5
+0.4
N.D.
N.D.
N.D.
lb
LB
+0.6
+0.3
N.D.
N.D.
N.D.
carbon source
butyrate (C)
+0.4
+0.6
N.D.
N.D.
N.D.
carbon source
Tween 20 (C)
+0.7
+0.4
N.D.
N.D.
N.D.
denitrifying
Nitrate reduction, replicate C t1
+0.7
+0.4
N.D.
N.D.
N.D.
stress
D-Cycloserine 0.1 mg/ml
+0.8
+0.4
N.D.
N.D.
N.D.
carbon source
D-Alanine (C)
+0.6
+0.6
N.D.
N.D.
N.D.
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