Fitness for 5 genes in Pseudomonas fluorescens FW300-N1B4

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500 ntPf1N1B4_403 and Pf1N1B4_404 are separated by 112 nucleotidesPf1N1B4_404 and Pf1N1B4_405 are separated by 135 nucleotidesPf1N1B4_405 and Pf1N1B4_406 overlap by 8 nucleotidesPf1N1B4_406 and Pf1N1B4_407 are separated by 220 nucleotides Pf1N1B4_403 - FIG00965925: hypothetical protein, at 472,414 to 472,668 _403 Pf1N1B4_404 - ParE toxin protein, at 472,781 to 473,161 _404 Pf1N1B4_405 - Carbohydrate kinase, PfkB family, at 473,297 to 474,226 _405 Pf1N1B4_406 - Purine-cytosine permease, at 474,219 to 475,685 _406 Pf1N1B4_407 - ADP-ribosylglycohydrolase YegU (EC 3.2.-.-), at 475,906 to 476,904 _407
Group Condition Pf1N1B4_403 Pf1N1B4_404 Pf1N1B4_405 Pf1N1B4_406 Pf1N1B4_407
temperature LB 37C -1.0 N.D. -1.3 -0.3 -2.1
carbon source carbon source Sucrose 1mM -0.3 N.D. -0.9 -0.2 -2.9
stress 1-ethyl-3-methylimidazolium chloride 80 mM -0.0 N.D. +0.7 -0.3 -3.3
stress nitrite 10 mM -0.4 N.D. -0.7 -0.5 -1.2
stress Benzethonium chloride 0.0125 mM -0.6 N.D. -0.2 -0.6 -1.1
nitrogen source D-Glucosamine Hydrochloride (N) -2.1 N.D. +0.3 -0.5 +0.1
carbon source acetate (C) -0.7 N.D. -0.1 +0.1 -1.4
carbon source L-Glutamic (C) -2.1 N.D. -0.1 +0.0 +0.2
carbon source carbon source Sucrose 0.25mM +0.7 N.D. -0.2 -0.9 -1.3
carbon source carbon source Sucrose 5mM -0.6 N.D. +0.3 +0.2 -1.3
stress Tetracycline 0.0004 mg/ml -0.5 N.D. +0.4 +0.2 -1.4
temperature LB 37C -1.4 N.D. +0.7 -0.1 +0.4
stress Gentamicin 0.001 mg/ml -0.9 N.D. -0.1 -0.3 +1.1
nitrogen source nitrate (N) +0.5 N.D. +0.6 +0.3 -1.6
stress D-Cycloserine 0.1 mg/ml -1.4 N.D. +0.1 +0.1 +1.2
carbon source octanoate (C) -1.3 N.D. -0.1 +0.3 +1.0
carbon source L-Citrulline (C) -0.9 N.D. -0.3 -0.1 +1.4
pH Minimal media pH9 +0.6 N.D. -1.1 +0.4 +0.4
nitrogen source L-Valine (N) -0.7 N.D. -0.3 -0.0 +1.4
carbon source carbon source Sucrose 20mM -1.2 N.D. +0.3 +0.3 +1.1
carbon source L-Glutamine (C) -1.0 N.D. +0.4 +0.1 +1.1
nitrogen source Carnitine Hydrochloride (N) -1.0 N.D. +0.4 +0.1 +1.2
carbon source casaminos (C) +0.6 N.D. -0.5 -0.1 +1.1
carbon source Uridine (C) -0.3 N.D. +0.4 -0.1 +1.6
denitrifying Denitrifying in presence of WT Pseudomonas N2-E3; replicate C; timepoint 1 +1.2 N.D. +0.5 -0.3 +0.4
carbon source L-Leucine (C) -0.1 N.D. -0.2 +0.0 +2.1
stress Fusidic 0.0625 mg/ml +0.2 N.D. -0.3 +0.3 +1.8
nitrogen source D-Alanine (N) +0.6 N.D. +0.3 -0.3 +1.5
denitrifying Nitrate reduction, replicate A +0.5 N.D. +0.4 -0.0 +1.9
denitrifying Denitrifying in presence of WT Pseudomonas N2-E3; replicate B; timepoint 1 +0.3 N.D. +0.0 +0.1 +2.4
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