Fitness for 5 genes in Pseudomonas fluorescens FW300-N1B4

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500 ntPf1N1B4_3998 and Pf1N1B4_3999 are separated by 240 nucleotidesPf1N1B4_3999 and Pf1N1B4_4000 overlap by 11 nucleotidesPf1N1B4_4000 and Pf1N1B4_4001 are separated by 15 nucleotidesPf1N1B4_4001 and Pf1N1B4_4002 are separated by 201 nucleotides Pf1N1B4_3998 - putative exported protein, at 4,443,602 to 4,447,276 _3998 Pf1N1B4_3999 - Transcriptional regulator, MarR family, at 4,447,517 to 4,447,996 _3999 Pf1N1B4_4000 - Outer membrane component of tripartite multidrug resistance system, at 4,447,986 to 4,449,455 _4000 Pf1N1B4_4001 - Membrane fusion component of tripartite multidrug resistance system, at 4,449,471 to 4,450,673 _4001 Pf1N1B4_4002 - Inner membrane component of tripartite multidrug resistance system, at 4,450,875 to 4,452,404 _4002
Group Condition Pf1N1B4_3998 Pf1N1B4_3999 Pf1N1B4_4000 Pf1N1B4_4001 Pf1N1B4_4002
pH Minimal media pH9 -3.9 N.D. -0.6 +0.2 -0.1
temperature LB 37C -2.1 N.D. -0.5 -1.5 -0.2
stress Aluminum chloride 2.5 mM -2.3 N.D. -0.3 -0.0 -0.4
denitrifying Denitrifying in presence of WT Pseudomonas N2-E3; replicate C; timepoint 1 -0.9 N.D. +0.3 -2.4 +0.2
pH Minimal media pH9 -2.1 N.D. +0.3 -0.6 -0.2
lb LB -0.4 N.D. -0.2 -1.8 -0.3
anaerobic N2E2wt with N1B4m denitrifying repA -2.3 N.D. +0.1 -0.5 +0.1
carbon source carbon source Sucrose 1mM -0.1 N.D. -0.5 -1.9 +0.1
stress Chloride 200 mM -0.6 N.D. +0.3 -1.8 -0.1
temperature LB 10C -1.4 N.D. +0.2 -0.9 -0.1
stress Cholin acetate 20 mM -1.9 N.D. +0.2 -0.4 -0.1
anaerobic N2E2wt with N1B4m denitrifying repB -1.8 N.D. +0.1 -0.1 -0.1
stress Chloride 300 mM -0.8 N.D. +0.2 -1.0 -0.3
carbon source carbon source Sucrose 0.25mM -0.9 N.D. -0.1 -0.9 +0.0
anaerobic N2E2wt with N1B4m denitrifying repA -1.4 N.D. -0.1 -0.4 +0.1
stress acetate 20 mM -1.3 N.D. +0.2 -0.5 -0.1
pH Minimal media pH8 -1.6 N.D. +0.2 -0.2 -0.1
stress Cholin acetate 10 mM -1.3 N.D. +0.5 -0.3 -0.3
denitrifying Denitrifying in presence of WT Pseudomonas N2-E3; replicate B; timepoint 1 -0.7 N.D. +0.1 -1.1 +0.3
stress Bacitracin 2 mg/ml -0.6 N.D. +0.3 -1.1 +0.2
stress copper (II) chloride 2.5 mM -1.3 N.D. -0.2 +0.5 -0.0
nitrogen source L-Histidine (N) -0.3 N.D. +0.3 -1.2 +0.2
denitrifying Nitrate reduction, replicate B -0.8 N.D. +0.5 -1.0 +0.4
nitrogen source D-Glucosamine Hydrochloride (N) +0.5 N.D. +0.6 -1.4 -0.4
carbon source octanoate (C) +1.3 N.D. -2.1 -0.1 +0.3
carbon source carbon source Sucrose 5mM +1.2 N.D. -0.4 -0.8 +0.0
nitrogen source Ammonium chloride (N) -0.4 N.D. -0.5 +0.9 +0.2
temperature LB 37C -0.7 N.D. -0.2 +1.0 +0.3
stress Doxycycline hyclate 0.0001 mg/ml +2.6 N.D. -0.1 -1.4 +0.1
nitrogen source nitrogen source nitrite 1mM +1.2 N.D. +0.3 +0.3 -0.2
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