Fitness for 5 genes in Pseudomonas fluorescens FW300-N1B4

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500 ntPf1N1B4_3996 and Pf1N1B4_3997 are separated by 65 nucleotidesPf1N1B4_3997 and Pf1N1B4_3998 overlap by 4 nucleotidesPf1N1B4_3998 and Pf1N1B4_3999 are separated by 240 nucleotidesPf1N1B4_3999 and Pf1N1B4_4000 overlap by 11 nucleotides Pf1N1B4_3996 - Histone acetyltransferase HPA2 and related acetyltransferases, at 4,441,162 to 4,441,812 _3996 Pf1N1B4_3997 - Uncharacterized protein YtfM precursor, at 4,441,878 to 4,443,605 _3997 Pf1N1B4_3998 - putative exported protein, at 4,443,602 to 4,447,276 _3998 Pf1N1B4_3999 - Transcriptional regulator, MarR family, at 4,447,517 to 4,447,996 _3999 Pf1N1B4_4000 - Outer membrane component of tripartite multidrug resistance system, at 4,447,986 to 4,449,455 _4000
Group Condition Pf1N1B4_3996 Pf1N1B4_3997 Pf1N1B4_3998 Pf1N1B4_3999 Pf1N1B4_4000
pH Minimal media pH9 +0.2 -4.0 -3.9 N.D. -0.6
pH Minimal media pH9 -1.8 -4.1 -2.1 N.D. +0.3
stress Aluminum chloride 2.5 mM -0.4 -2.7 -2.3 N.D. -0.3
temperature LB 37C +0.0 -2.4 -2.1 N.D. -0.5
anaerobic N2E2wt with N1B4m denitrifying repA -0.1 -2.2 -2.3 N.D. +0.1
anaerobic N2E2wt with N1B4m denitrifying repA -0.5 -2.2 -1.4 N.D. -0.1
anaerobic N2E2wt with N1B4m denitrifying repB -0.5 -1.3 -1.8 N.D. +0.1
stress 1-ethyl-3-methylimidazolium lysine 5 mM -0.1 -1.9 -1.3 N.D. +0.1
stress Choline Lysine 6 mM +0.2 -2.1 -1.2 N.D. -0.0
temperature LB 37C +0.7 -2.9 -0.7 N.D. -0.2
pH Minimal media pH8 +0.1 -1.5 -1.6 N.D. +0.2
stress Fusidic 0.125 mg/ml +0.2 -1.6 -0.9 N.D. -0.3
stress Spectinomycin 0.05 mg/ml -0.6 -1.1 -0.8 N.D. -0.1
temperature LB 10C -0.0 -1.2 -1.4 N.D. +0.2
stress Cholin acetate 20 mM +0.1 -0.8 -1.9 N.D. +0.2
carbon source acetate (C) -0.2 -1.0 -0.9 N.D. -0.1
carbon source acetate (C) -0.1 -1.2 -0.9 N.D. -0.1
carbon source carbon source Sucrose 0.25mM -0.7 -0.3 -0.9 N.D. -0.1
stress acetate 20 mM -0.0 -0.9 -1.3 N.D. +0.2
stress Cholin acetate 10 mM +0.2 -1.3 -1.3 N.D. +0.5
denitrifying Denitrifying in presence of WT Pseudomonas N2-E3; replicate C; timepoint 1 -0.6 -0.2 -0.9 N.D. +0.3
carbon source L-Citrulline (C) +0.2 -0.9 -0.8 N.D. +0.1
temperature LB 30C +0.3 -0.6 -1.0 N.D. +0.2
stress methylglyoxal 0.008 vol% +0.6 -0.2 -0.8 N.D. +0.6
stress Cisplatin 0.006 mg/ml -0.6 +0.4 +1.0 N.D. -0.1
carbon source octanoate (C) +0.8 +1.7 +1.3 N.D. -2.1
nitrogen source Cytidine (N) +1.0 +0.8 +0.5 N.D. -0.6
carbon source carbon source Sucrose 5mM +1.0 +1.3 +1.2 N.D. -0.4
nitrogen source nitrogen source nitrite 1mM +0.6 +0.9 +1.2 N.D. +0.3
stress Doxycycline hyclate 0.0001 mg/ml +1.5 +2.0 +2.6 N.D. -0.1
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