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Fitness for 5 genes in
Pseudomonas fluorescens FW300-N1B4
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500 nt
Pf1N1B4_3772 and Pf1N1B4_3773 are separated by 102 nucleotides
Pf1N1B4_3773 and Pf1N1B4_3774 are separated by 77 nucleotides
Pf1N1B4_3774 and Pf1N1B4_3775 are separated by 75 nucleotides
Pf1N1B4_3775 and Pf1N1B4_3776 are separated by 131 nucleotides
Pf1N1B4_3772 - Glutaredoxin, at 4,183,047 to 4,183,418
_3772
Pf1N1B4_3773 - GTP cyclohydrolase I (EC 3.5.4.16) type 1, at 4,183,521 to 4,184,066
_3773
Pf1N1B4_3774 - Smr domain protein, at 4,184,144 to 4,184,701
_3774
Pf1N1B4_3775 - FIG00956876: hypothetical protein, at 4,184,777 to 4,185,097
_3775
Pf1N1B4_3776 - Isochorismatase (EC 3.3.2.1), at 4,185,229 to 4,185,819
_3776
Group
Condition
Pf1N1B4
_3772
Pf1N1B4
_3773
Pf1N1B4
_3774
Pf1N1B4
_3775
Pf1N1B4
_3776
nitrogen source
nitrogen source nitrite 0.1mM
-0.4
N.D.
N.D.
-2.2
N.D.
stress
Fusidic 0.0625 mg/ml
+0.4
N.D.
N.D.
-2.8
N.D.
nitrogen source
nitrogen source nitrate 20mM
-0.8
N.D.
N.D.
-1.4
N.D.
anaerobic
N2E2wt with N1B4m denitrifying repA
+0.1
N.D.
N.D.
-2.0
N.D.
denitrifying
Nitrate reduction, replicate C
+0.0
N.D.
N.D.
-1.9
N.D.
stress
Aluminum chloride 2.5 mM
+0.1
N.D.
N.D.
-1.9
N.D.
denitrifying
Nitrate reduction, replicate A
+0.1
N.D.
N.D.
-1.8
N.D.
nitrogen source
L-Methionine (N)
-0.1
N.D.
N.D.
-1.5
N.D.
stress
Cholin acetate 20 mM
+0.2
N.D.
N.D.
-1.8
N.D.
nitrogen source
L-Citrulline (N)
-0.1
N.D.
N.D.
-1.2
N.D.
stress
Chlorite 1 mM
-0.1
N.D.
N.D.
-1.2
N.D.
denitrifying
Nitrate reduction, replicate C t1
-0.3
N.D.
N.D.
-1.1
N.D.
anaerobic
N2E2wt with N1B4m denitrifying repB
+0.3
N.D.
N.D.
-1.6
N.D.
carbon source
carbon source Sucrose 0.25mM
-1.7
N.D.
N.D.
+0.6
N.D.
stress
Dimethyl Sulfoxide 6.5 vol%
+0.2
N.D.
N.D.
-1.3
N.D.
anaerobic
Nitrate reduction
-1.2
N.D.
N.D.
+0.2
N.D.
stress
Tetracycline 0.0004 mg/ml
+0.2
N.D.
N.D.
-1.1
N.D.
carbon source
carbon source Sucrose 1mM
+0.9
N.D.
N.D.
-1.8
N.D.
lb
LB
+0.3
N.D.
N.D.
-1.1
N.D.
lb
LB
+0.4
N.D.
N.D.
-1.2
N.D.
stress
Carbenicillin 2 mg/ml
+0.3
N.D.
N.D.
-1.1
N.D.
stress
Cisplatin 0.006 mg/ml
+0.7
N.D.
N.D.
-1.4
N.D.
stress
Thallium(I) acetate 0.008 mg/ml
+0.4
N.D.
N.D.
-0.9
N.D.
denitrifying
Denitrifying in presence of WT Pseudomonas N2-E3; replicate C; timepoint 1
+0.5
N.D.
N.D.
-1.1
N.D.
stress
methylglyoxal 0.008 vol%
+0.4
N.D.
N.D.
-0.9
N.D.
stress
Gentamicin 0.001 mg/ml
+0.5
N.D.
N.D.
-0.8
N.D.
nitrogen source
Thymine (N)
+0.4
N.D.
N.D.
-0.8
N.D.
stress
Bacitracin 2 mg/ml
+0.6
N.D.
N.D.
-0.9
N.D.
stress
Chloride 300 mM
+0.6
N.D.
N.D.
+0.6
N.D.
pH
Minimal media pH9
+0.8
N.D.
N.D.
+0.8
N.D.
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