Fitness for 5 genes in Pseudomonas fluorescens FW300-N1B4

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500 ntPf1N1B4_3658 and Pf1N1B4_3659 are separated by 174 nucleotidesPf1N1B4_3659 and Pf1N1B4_3660 are separated by 269 nucleotidesPf1N1B4_3660 and Pf1N1B4_3661 are separated by 128 nucleotidesPf1N1B4_3661 and Pf1N1B4_3662 are separated by 6 nucleotides Pf1N1B4_3658 - D-glycerate 2-kinase (EC 2.7.1.-), at 4,063,141 to 4,064,421 _3658 Pf1N1B4_3659 - 2-hydroxy-3-oxopropionate reductase (EC 1.1.1.60), at 4,064,596 to 4,065,366 _3659 Pf1N1B4_3660 - Hydroxypyruvate isomerase (EC 5.3.1.22), at 4,065,636 to 4,066,418 _3660 Pf1N1B4_3661 - Glyoxylate carboligase (EC 4.1.1.47), at 4,066,547 to 4,068,322 _3661 Pf1N1B4_3662 - hypothetical protein, at 4,068,329 to 4,068,592 _3662
Group Condition Pf1N1B4_3658 Pf1N1B4_3659 Pf1N1B4_3660 Pf1N1B4_3661 Pf1N1B4_3662
stress Fusidic 0.125 mg/ml -0.2 N.D. -0.6 -0.4 -0.4
nitrogen source nitrogen source nitrite 0.5mM -0.4 N.D. -0.4 -0.3 -0.4
carbon source L-Arginine (C) -0.0 N.D. -0.1 -0.2 -1.2
carbon source L-Isoleucine (C) -0.5 N.D. -0.0 -0.2 -0.5
carbon source L-Valine (C) +0.1 N.D. -0.1 -0.2 -0.9
carbon source Cytidine (C) -0.3 N.D. +0.2 -0.3 -0.5
carbon source m-Inositol (C) +0.1 N.D. -0.2 -0.4 -0.5
carbon source carbon source Sucrose 0.5mM +0.3 N.D. +0.1 -0.2 -1.3
nitrogen source nitrogen source nitrite 0.5mM -0.3 N.D. +0.1 -0.2 -0.6
denitrifying Nitrate reduction, replicate C +0.1 N.D. -0.5 -0.2 -0.4
temperature LB 37C -1.0 N.D. -0.4 +0.1 +0.5
carbon source carbon source Sucrose 5mM -0.1 N.D. +0.3 -0.4 -0.4
stress nitrate 100 mM +0.1 N.D. -0.9 +0.3 -0.0
carbon source carbon source Sucrose 0.25mM -0.6 N.D. +0.1 +0.8 -0.6
denitrifying Nitrate reduction, replicate B +0.3 N.D. +0.1 -0.3 -0.4
stress Doxycycline hyclate 0.0001 mg/ml -0.4 N.D. +0.2 +0.5 -0.4
carbon source carbon source Sucrose 1mM -0.3 N.D. +0.2 +0.3 -0.3
pH Minimal media pH9 +0.3 N.D. +0.3 +0.1 -0.7
denitrifying Denitrifying in presence of WT Pseudomonas N2-E3; replicate C; timepoint 1 -0.4 N.D. +0.4 -0.3 +0.4
stress Thallium(I) acetate 0.008 mg/ml +0.4 N.D. +0.3 +0.1 -0.5
nitrogen source Thymine (N) +0.4 N.D. -0.4 -0.3 +0.7
carbon source carbon source Sucrose 0.25mM +0.0 N.D. +0.8 -0.0 -0.4
carbon source D-Glucose (C) -0.2 N.D. +0.1 +0.3 +0.4
carbon source carbon source Sucrose 20mM +0.0 N.D. +0.7 +0.3 -0.4
carbon source pyruvate (C) +0.4 N.D. +0.6 +0.0 -0.3
nitrogen source Cytidine (N) +0.2 N.D. +0.3 -0.3 +0.6
stress Carbenicillin 2 mg/ml +0.5 N.D. +0.1 +0.5 -0.2
stress copper (II) chloride 2.5 mM -0.1 N.D. +0.8 +0.5 -0.0
nitrogen source Ammonium chloride (N) +0.8 N.D. +0.1 -0.1 +0.3
carbon source D-Glucose (C) +0.2 N.D. +0.3 +0.3 +0.4
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