Fitness for 5 genes in Pseudomonas fluorescens FW300-N1B4

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500 ntPf1N1B4_3651 and Pf1N1B4_3652 are separated by 289 nucleotidesPf1N1B4_3652 and Pf1N1B4_3653 are separated by 130 nucleotidesPf1N1B4_3653 and Pf1N1B4_3654 are separated by 185 nucleotidesPf1N1B4_3654 and Pf1N1B4_3655 are separated by 106 nucleotides Pf1N1B4_3651 - Antitoxin 1, at 4,055,487 to 4,055,891 _3651 Pf1N1B4_3652 - Mobile element protein, at 4,056,181 to 4,057,209 _3652 Pf1N1B4_3653 - Major facilitator family transporter, at 4,057,340 to 4,058,545 _3653 Pf1N1B4_3654 - Sulfate and thiosulfate binding protein CysP, at 4,058,731 to 4,059,729 _3654 Pf1N1B4_3655 - Potassium voltage-gated channel subfamily KQT; possible potassium channel, VIC family, at 4,059,836 to 4,060,660 _3655
Group Condition Pf1N1B4_3651 Pf1N1B4_3652 Pf1N1B4_3653 Pf1N1B4_3654 Pf1N1B4_3655
carbon source carbon source Sucrose 0.5mM N.D. N.D. -0.8 -0.3 -0.3
nitrogen source L-Methionine (N) N.D. N.D. +0.4 -0.7 -0.6
lb LB N.D. N.D. -0.2 -0.2 -0.3
temperature LB 37C N.D. N.D. -0.7 +0.2 -0.1
stress Bacitracin 2 mg/ml N.D. N.D. +0.2 -0.3 -0.2
carbon source carbon source Sucrose 5mM N.D. N.D. -0.5 +0.4 -0.2
stress Chlorite 1 mM N.D. N.D. +0.2 -0.4 +0.1
stress Benzethonium chloride 0.0125 mM N.D. N.D. +0.2 +0.2 -0.4
stress Chloride 200 mM N.D. N.D. +0.3 -0.0 -0.3
nitrogen source L-Citrulline (N) N.D. N.D. +0.4 -0.2 -0.1
anaerobic N2E2wt with N1B4m denitrifying repA N.D. N.D. +0.2 +0.2 -0.4
carbon source a-Ketoglutaric (C) N.D. N.D. -0.3 +0.3 +0.2
stress Chloride 300 mM N.D. N.D. +0.2 +0.2 -0.2
carbon source Fumarate (C) N.D. N.D. -0.1 -0.1 +0.4
stress Fusidic 0.125 mg/ml N.D. N.D. +0.7 -0.3 -0.2
nitrogen source D-Glucosamine Hydrochloride (N) N.D. N.D. +0.3 -0.3 +0.2
nitrogen source L-Isoleucine (N) N.D. N.D. +0.3 -0.3 +0.2
stress Thallium(I) acetate 0.008 mg/ml N.D. N.D. +0.3 -0.3 +0.2
nitrogen source D-Alanine (N) N.D. N.D. +0.4 -0.2 +0.2
carbon source carbon source Sucrose 1mM N.D. N.D. +0.7 -0.1 -0.0
pH Minimal media pH9 N.D. N.D. -0.4 +0.4 +0.7
carbon source D-Trehalose (C) N.D. N.D. +0.5 +0.0 +0.2
denitrifying Denitrifying in presence of WT Pseudomonas N2-E3; replicate C; timepoint 1 N.D. N.D. +0.7 -0.0 -0.0
carbon source D-Galacturonic Acid (C) N.D. N.D. +0.4 -0.1 +0.4
stress Paraquat dichloride 0.016 mg/mL N.D. N.D. +0.5 +0.2 +0.1
stress Fusidic 0.0625 mg/ml N.D. N.D. +0.4 +0.1 +0.3
carbon source Fumarate (C) N.D. N.D. +0.3 +0.0 +0.6
pH Minimal media pH9 N.D. N.D. -0.0 +0.3 +0.7
carbon source carbon source Sucrose 1mM N.D. N.D. +0.5 +0.3 +0.3
stress Doxycycline hyclate 0.0001 mg/ml N.D. N.D. +0.9 -0.0 +0.6
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