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Cofit
Protein
Homologs
Fitness for 5 genes in
Pseudomonas fluorescens FW300-N1B4
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500 nt
Pf1N1B4_362 and Pf1N1B4_363 overlap by 8 nucleotides
Pf1N1B4_363 and Pf1N1B4_364 are separated by 3 nucleotides
Pf1N1B4_364 and Pf1N1B4_365 are separated by 286 nucleotides
Pf1N1B4_365 and Pf1N1B4_366 are separated by 12 nucleotides
Pf1N1B4_362 - Lipid A export ATP-binding/permease protein MsbA, at 430,596 to 432,413
_362
Pf1N1B4_363 - Thioredoxin, at 432,406 to 433,044
_363
Pf1N1B4_364 - Rhodanese domain protein UPF0176, at 433,048 to 433,989
_364
Pf1N1B4_365 - Cell division protein BolA, at 434,276 to 434,575
_365
Pf1N1B4_366 - FIG00954136: hypothetical protein, at 434,588 to 435,109
_366
Group
Condition
Pf1N1B4
_362
Pf1N1B4
_363
Pf1N1B4
_364
Pf1N1B4
_365
Pf1N1B4
_366
carbon source
D-Lactate (C)
+0.0
-0.3
-0.8
-2.3
-1.0
carbon source
D-Lactate (C)
-0.1
+0.2
-0.6
-2.6
-1.0
carbon source
D,L-Lactate (C)
-0.2
-0.2
-1.0
-1.5
-0.8
carbon source
D,L-Lactate (C)
-0.2
-0.0
-0.9
-1.3
-0.7
stress
Paraquat dichloride 0.016 mg/mL
-0.2
-0.9
-0.2
-0.9
-0.5
carbon source
L-Lactate (C)
-0.2
+0.0
-0.4
-1.4
-0.5
carbon source
L-Lactate (C)
+0.3
-0.1
-0.1
-1.6
-1.0
stress
Carbenicillin 2 mg/ml
-0.3
-0.5
-0.7
-0.2
-0.6
carbon source
L-Histidine (C)
+0.0
-0.3
-1.4
-0.4
-0.2
stress
Thallium(I) acetate 0.008 mg/ml
-0.2
+0.0
-1.1
-1.0
-0.1
carbon source
carbon source Sucrose 5mM
+0.3
-0.5
-0.5
-1.4
-0.0
stress
D-Cycloserine 0.1 mg/ml
+0.0
-0.7
-0.2
-0.7
-0.6
carbon source
carbon source Sucrose 0.25mM
+0.0
-1.1
-0.8
-0.9
+0.7
stress
nitrite 10 mM
+0.2
-0.4
-1.4
-0.4
-0.0
stress
Aluminum chloride 2.5 mM
+0.2
-0.5
-0.2
-0.2
-1.3
nitrogen source
Uridine (N)
+0.1
+0.5
-0.8
-1.1
-0.4
carbon source
Glycerol (C)
+0.1
-0.1
-0.2
-1.3
-0.4
carbon source
carbon source Sucrose 5mM
+0.0
+0.1
-1.9
+0.0
+0.1
anaerobic
Nitrate reduction
-0.2
+0.4
-0.9
-0.6
-0.1
carbon source
carbon source Sucrose 1mM
+0.3
-0.1
-0.9
-0.9
+0.6
denitrifying
Denitrifying in presence of WT Pseudomonas N2-E3; replicate C; timepoint 1
+0.2
-0.1
-1.4
+0.6
-0.2
carbon source
carbon source Sucrose 0.25mM
+0.3
+0.2
-1.8
-0.3
+0.9
carbon source
carbon source Sucrose 0.5mM
+0.0
+0.4
-0.9
-0.9
+0.8
carbon source
carbon source Sucrose 1mM
-0.2
+0.3
-0.8
-0.5
+0.6
stress
Rifampicin 0.004 mg/ml
+0.0
-0.4
-0.6
+0.3
+0.7
pH
Minimal media pH9
+0.1
+0.0
-1.3
+0.6
+1.1
pH
Minimal media pH9
-0.3
+0.6
-0.7
+0.5
+1.4
nitrogen source
nitrogen source nitrite 0.1mM
+0.6
+0.8
+0.3
-0.4
+0.4
stress
Benzethonium chloride 0.0125 mM
+0.1
+0.7
+0.6
+0.5
+0.5
stress
Doxycycline hyclate 0.0001 mg/ml
+0.2
+0.7
-0.5
+1.3
+1.4
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