Fitness for 5 genes in Pseudomonas fluorescens FW300-N1B4

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500 ntPf1N1B4_3485 and Pf1N1B4_3486 are separated by 126 nucleotidesPf1N1B4_3486 and Pf1N1B4_3487 are separated by 340 nucleotidesPf1N1B4_3487 and Pf1N1B4_3488 are separated by 145 nucleotidesPf1N1B4_3488 and Pf1N1B4_3489 are separated by 136 nucleotides Pf1N1B4_3485 - hypothetical protein, at 3,892,525 to 3,892,884 _3485 Pf1N1B4_3486 - Transcriptional regulator, AraC family, at 3,893,011 to 3,894,024 _3486 Pf1N1B4_3487 - Aerobic C4-dicarboxylate transporter for fumarate, L-malate, D-malate, succunate, aspartate, at 3,894,365 to 3,895,699 _3487 Pf1N1B4_3488 - Alpha-methylacyl-CoA racemase (EC 5.1.99.4), at 3,895,845 to 3,897,026 _3488 Pf1N1B4_3489 - Protein sprT, at 3,897,163 to 3,897,657 _3489
Group Condition Pf1N1B4_3485 Pf1N1B4_3486 Pf1N1B4_3487 Pf1N1B4_3488 Pf1N1B4_3489
carbon source carbon source Sucrose 5mM -2.0 +0.3 -0.9 -1.0 +0.2
anaerobic Nitrate reduction -1.5 -0.7 -0.4 +0.1 -0.6
carbon source carbon source Sucrose 1mM -0.8 -0.4 +0.3 +0.0 -1.4
carbon source D-Ribose (C) -1.4 -0.3 -0.4 -0.1 +0.3
nitrogen source D-Glucosamine Hydrochloride (N) -0.5 -0.7 -0.4 -0.2 +0.5
carbon source Fumarate (C) +0.4 +0.4 -2.1 -0.1 +0.3
carbon source L-Malic (C) +0.3 -0.1 -1.2 -0.2 +0.1
carbon source Fumarate (C) +0.3 +0.2 -2.1 -0.0 +0.6
nitrogen source L-Methionine (N) -1.2 +0.3 +0.5 -0.3 -0.0
carbon source carbon source Sucrose 20mM -1.6 -0.2 +0.4 +0.0 +0.7
pH Minimal media pH9 +0.2 -0.8 +0.8 -0.3 +0.0
nitrogen source nitrogen source nitrite 0.1mM +0.7 -0.8 -0.3 +0.2 +0.2
carbon source carbon source Sucrose 1mM +0.8 -0.7 +0.3 +0.0 +0.1
carbon source pyruvate (C) +0.8 +0.1 -0.5 -0.3 +0.3
carbon source carbon source Sucrose 0.25mM -0.7 +0.7 -0.6 +0.6 +0.6
nitrogen source L-Leucine (N) +1.0 -0.5 +0.1 +0.0 +0.4
nitrogen source nitrogen source nitrite 0.1mM +0.7 +0.0 -0.3 -0.2 +0.8
carbon source carbon source Sucrose 0.25mM -0.3 +1.7 -0.4 +0.6 -0.5
denitrifying Denitrifying in presence of WT Pseudomonas N2-E3; replicate C; timepoint 1 +1.3 +0.3 -0.3 -0.1 -0.2
carbon source octanoate (C) +0.9 -0.4 +0.6 +0.0 +0.0
pH Minimal media pH9 +0.5 +0.2 +0.8 -0.1 -0.1
nitrogen source L-Citrulline (N) +1.1 -0.2 +0.1 -0.0 +0.3
carbon source L-Glutamic (C) +0.8 -0.3 +0.2 -0.0 +0.6
carbon source L-Leucine (C) +0.8 -0.2 +0.5 -0.1 +0.3
lb LB +0.8 +0.4 +0.0 -0.3 +0.6
anaerobic N2E2wt with N1B4m denitrifying repB +1.1 -0.3 +0.4 -0.0 +0.5
stress acetate 20 mM +1.0 +0.4 +0.3 -0.1 +0.2
stress Gentamicin 0.001 mg/ml +1.0 +0.3 +0.6 -0.2 -0.1
carbon source m-Inositol (C) +0.9 -0.2 +0.7 +0.1 +0.4
stress Sisomicin 0.001 mg/ml +0.7 +1.0 +0.7 -0.4 -0.1
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