Fitness for 5 genes in Pseudomonas fluorescens FW300-N1B4

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500 ntPf1N1B4_3397 and Pf1N1B4_3398 are separated by 100 nucleotidesPf1N1B4_3398 and Pf1N1B4_3399 are separated by 76 nucleotidesPf1N1B4_3399 and Pf1N1B4_3400 are separated by 139 nucleotidesPf1N1B4_3400 and Pf1N1B4_3401 are separated by 89 nucleotides Pf1N1B4_3397 - Ketosteroid isomerase homolog, at 3,790,184 to 3,790,609 _3397 Pf1N1B4_3398 - DGPFAETKE, at 3,790,710 to 3,791,054 _3398 Pf1N1B4_3399 - RNA polymerase sigma-70 factor, ECF subfamily, at 3,791,131 to 3,792,366 _3399 Pf1N1B4_3400 - LysR-family transcriptional regulator STM3020, at 3,792,506 to 3,793,360 _3400 Pf1N1B4_3401 - FIG00961830: hypothetical protein, at 3,793,450 to 3,793,887 _3401
Group Condition Pf1N1B4_3397 Pf1N1B4_3398 Pf1N1B4_3399 Pf1N1B4_3400 Pf1N1B4_3401
carbon source carbon source Sucrose 0.25mM N.D. N.D. -1.4 N.D. -0.4
stress Cholin acetate 10 mM N.D. N.D. -0.6 N.D. -0.5
stress methylglyoxal 0.004 vol% N.D. N.D. -0.6 N.D. -0.5
nitrogen source D-Glucosamine Hydrochloride (N) N.D. N.D. -0.8 N.D. -0.3
stress nitrate 100 mM N.D. N.D. -0.7 N.D. -0.1
lb LB N.D. N.D. -0.5 N.D. -0.3
temperature LB 37C N.D. N.D. +0.1 N.D. -0.9
stress Nalidixic 0.004 mg/ml N.D. N.D. -0.6 N.D. -0.2
anaerobic N2E2wt with N1B4m denitrifying repB N.D. N.D. -0.4 N.D. -0.4
stress Sisomicin 0.001 mg/ml N.D. N.D. -0.7 N.D. +0.2
nitrogen source Adenine (N) N.D. N.D. +0.3 N.D. -0.5
nitrogen source Thymine (N) N.D. N.D. -0.5 N.D. +0.3
carbon source carbon source Sucrose 5mM N.D. N.D. +0.3 N.D. -0.4
carbon source L-Histidine (C) N.D. N.D. +0.3 N.D. -0.4
carbon source L-Alanine (C) N.D. N.D. +0.5 N.D. -0.5
denitrifying Denitrifying in presence of WT Pseudomonas N2-E3; replicate C; timepoint 1 N.D. N.D. -0.6 N.D. +0.6
carbon source D-Galacturonic Acid (C) N.D. N.D. +0.4 N.D. -0.3
nitrogen source D-Alanine (N) N.D. N.D. +0.6 N.D. -0.4
nitrogen source L-Isoleucine (N) N.D. N.D. +0.5 N.D. -0.4
carbon source L-Arabinose (C) N.D. N.D. +0.5 N.D. -0.3
carbon source L-Glutamic (C) N.D. N.D. +0.6 N.D. -0.3
denitrifying Nitrate reduction, replicate B t1 N.D. N.D. +0.5 N.D. -0.2
nitrogen source L-Citrulline (N) N.D. N.D. +0.6 N.D. -0.2
carbon source Cytidine (C) N.D. N.D. +0.7 N.D. -0.2
stress Dimethyl Sulfoxide 5 vol% N.D. N.D. +0.7 N.D. -0.1
denitrifying Denitrifying in presence of WT Pseudomonas N2-E3; replicate B; timepoint 1 N.D. N.D. +0.7 N.D. -0.1
nitrogen source L-Methionine (N) N.D. N.D. +0.9 N.D. -0.0
nitrogen source nitrogen source nitrate 20mM N.D. N.D. +0.7 N.D. +0.2
stress Spectinomycin 0.05 mg/ml N.D. N.D. +0.6 N.D. +0.3
stress Cisplatin 0.006 mg/ml N.D. N.D. +0.5 N.D. +0.6
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