Fitness for 5 genes in Pseudomonas fluorescens FW300-N1B4

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500 ntPf1N1B4_5219 and Pf1N1B4_3296 are separated by 181 nucleotidesPf1N1B4_3296 and Pf1N1B4_3297 are separated by 314 nucleotidesPf1N1B4_3297 and Pf1N1B4_3298 are separated by 32 nucleotidesPf1N1B4_3298 and Pf1N1B4_3299 overlap by 2 nucleotides Pf1N1B4_5219 - tRNA-Gly-CCC, at 3,683,127 to 3,683,197 _5219 Pf1N1B4_3296 - (R)-3-hydroxydecanoyl-ACP:CoA transacylase PhaG (3-hydroxyacyl-CoA-acyl carrier protein transferase) (EC 2.4.1.-), at 3,683,379 to 3,684,263 _3296 Pf1N1B4_3297 - Ribosomal small subunit pseudouridine synthase A (EC 4.2.1.70), at 3,684,578 to 3,685,270 _3297 Pf1N1B4_3298 - DNA or RNA helicases of superfamily II, at 3,685,303 to 3,685,521 _3298 Pf1N1B4_3299 - hypothetical protein, at 3,685,520 to 3,685,660 _3299
Group Condition Pf1N1B4_5219 Pf1N1B4_3296 Pf1N1B4_3297 Pf1N1B4_3298 Pf1N1B4_3299
carbon source carbon source Sucrose 0.5mM -3.5 +0.2 -0.2 N.D. N.D.
nitrogen source nitrogen source nitrite 0.5mM -1.2 -1.8 -0.2 N.D. N.D.
carbon source carbon source Sucrose 5mM -1.4 -0.4 -1.2 N.D. N.D.
anaerobic N2E2wt with N1B4m denitrifying repA -0.8 -1.7 -0.4 N.D. N.D.
nitrogen source nitrogen source nitrite 1mM -0.9 -1.0 -0.1 N.D. N.D.
carbon source carbon source Sucrose 5mM -1.6 -0.4 +0.0 N.D. N.D.
carbon source carbon source Sucrose 1mM -0.7 -0.5 -0.8 N.D. N.D.
nitrogen source nitrogen source nitrite 0.5mM +0.1 -2.1 +0.3 N.D. N.D.
carbon source acetate (C) -0.6 -0.6 -0.5 N.D. N.D.
stress Dimethyl Sulfoxide 6.5 vol% -1.0 -0.8 +0.2 N.D. N.D.
stress copper (II) chloride 2.5 mM -0.6 +0.1 -1.0 N.D. N.D.
temperature LB 37C -1.3 +0.1 -0.3 N.D. N.D.
stress Tetracycline 0.0004 mg/ml -0.7 -0.8 +0.2 N.D. N.D.
nitrogen source Putrescine (N) -1.3 -0.4 +0.3 N.D. N.D.
nitrogen source Thymine (N) -1.6 +0.5 -0.1 N.D. N.D.
carbon source L-Alanine (C) -1.5 +0.3 +0.0 N.D. N.D.
carbon source D-Galactose (C) -1.1 +0.3 -0.2 N.D. N.D.
nitrogen source Uridine (N) -0.9 +0.5 -0.6 N.D. N.D.
nitrogen source Cytidine (N) -1.4 +1.0 -0.6 N.D. N.D.
lb LB -1.5 +0.1 +0.3 N.D. N.D.
nitrogen source nitrogen source nitrate 20mM -0.9 -0.5 +0.7 N.D. N.D.
carbon source carbon source Sucrose 1mM -1.5 +0.3 +0.7 N.D. N.D.
pH Minimal media pH9 -0.9 +0.6 -0.1 N.D. N.D.
nitrogen source nitrogen source nitrite 0.1mM +0.8 -1.3 +0.2 N.D. N.D.
denitrifying Nitrate reduction in presence of wild-type Pseudomonas N2-E3; replicate A -0.9 +0.5 +0.3 N.D. N.D.
nitrogen source Ammonium chloride (N) -0.6 +0.9 -0.3 N.D. N.D.
nitrogen source Adenine (N) -0.5 +1.1 -0.5 N.D. N.D.
nitrogen source nitrate (N) -0.6 +1.4 -0.4 N.D. N.D.
nitrogen source Cytosine (N) -0.5 +1.3 -0.4 N.D. N.D.
stress Choline Lysine 6 mM +1.1 +0.6 +0.1 N.D. N.D.
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