Fitness for 5 genes in Pseudomonas fluorescens FW300-N1B4

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500 ntPf1N1B4_3280 and Pf1N1B4_3281 are separated by 202 nucleotidesPf1N1B4_3281 and Pf1N1B4_3282 are separated by 126 nucleotidesPf1N1B4_3282 and Pf1N1B4_3283 are separated by 389 nucleotidesPf1N1B4_3283 and Pf1N1B4_3284 are separated by 56 nucleotides Pf1N1B4_3280 - Prophage Lp2 protein 6, at 3,667,401 to 3,668,486 _3280 Pf1N1B4_3281 - AidA, at 3,668,689 to 3,669,228 _3281 Pf1N1B4_3282 - AidA, at 3,669,355 to 3,669,858 _3282 Pf1N1B4_3283 - Trans-aconitate 2-methyltransferase (EC 2.1.1.144), at 3,670,248 to 3,671,018 _3283 Pf1N1B4_3284 - Antitoxin 1, at 3,671,075 to 3,671,482 _3284
Group Condition Pf1N1B4_3280 Pf1N1B4_3281 Pf1N1B4_3282 Pf1N1B4_3283 Pf1N1B4_3284
denitrifying Denitrifying in presence of WT Pseudomonas N2-E3; replicate C; timepoint 1 -0.2 -2.1 +0.1 -0.0 +0.2
denitrifying Nitrate reduction, replicate A -0.1 -2.1 +0.3 +0.2 -0.2
denitrifying Nitrate reduction, replicate B +0.1 -1.8 +0.5 -0.1 -0.6
denitrifying Nitrate reduction, replicate C -0.2 -2.1 +0.1 +0.3 +0.1
carbon source carbon source Sucrose 1mM -0.1 -0.6 +0.0 -0.2 -0.9
nitrogen source nitrogen source nitrite 1mM +0.3 -0.8 +0.3 +0.0 -1.7
denitrifying Nitrate reduction in presence of wild-type Pseudomonas N2-E3; replicate A +0.1 -0.7 -0.3 +0.0 -0.9
carbon source carbon source Sucrose 0.5mM -0.3 -0.1 -0.0 +0.3 -1.5
denitrifying Nitrate reduction, replicate C t1 +0.1 -1.6 -0.2 -0.0 +0.2
denitrifying Nitrate reduction, replicate A t2 +0.4 -1.7 +0.1 +0.1 -0.1
temperature LB 37C -0.4 +0.2 +0.6 -0.7 -0.9
anaerobic Nitrate reduction +0.3 -0.5 +0.1 -0.2 -1.0
carbon source carbon source Sucrose 0.25mM -0.2 +1.1 -0.3 +0.1 -1.9
denitrifying Nitrate reduction, replicate C t3 +0.4 -1.8 -0.2 +0.1 +0.3
nitrogen source nitrogen source nitrate 20mM -0.2 -0.8 +0.1 +0.1 -0.2
nitrogen source nitrogen source nitrite 0.5mM +0.5 -0.9 -0.0 +0.1 -0.4
carbon source L-Arabinose (C) +0.3 -0.3 +0.0 +0.0 -0.8
carbon source carbon source Sucrose 5mM +0.4 +0.1 -0.7 +0.0 -0.4
denitrifying Nitrate reduction, replicate C t2 +0.1 -0.9 -0.2 +0.1 +0.4
nitrogen source D-Glucosamine Hydrochloride (N) -0.1 -0.6 -0.5 +0.2 +0.6
stress sodium fluoride 12.5 mM +0.3 -0.7 -0.3 +0.0 +0.3
nitrogen source nitrogen source Ammonium chloride 20mM +0.2 +0.4 -0.4 +0.0 -0.5
carbon source carbon source Sucrose 0.25mM +0.2 +0.6 +0.0 +0.4 -1.3
stress Aluminum chloride 2.5 mM +0.3 -0.7 +0.2 +0.1 +0.2
stress Spectinomycin 0.025 mg/ml -0.4 -0.2 +0.2 -0.1 +0.5
stress Benzethonium chloride 0.0125 mM -0.1 +0.0 +0.6 +0.2 -0.5
nitrogen source Ammonium chloride (N) +0.3 +0.7 -0.1 -0.1 -0.3
stress Fusidic 0.0625 mg/ml +0.2 -0.3 +0.3 +0.3 +0.2
nitrogen source Uridine (N) +0.4 +0.9 -0.2 +0.1 -0.4
denitrifying Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate A +0.2 +0.3 +0.4 -0.2 +0.4
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