Fitness for 5 genes in Pseudomonas fluorescens FW300-N1B4

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500 ntPf1N1B4_3269 and Pf1N1B4_3270 are separated by 156 nucleotidesPf1N1B4_3270 and Pf1N1B4_3271 overlap by 4 nucleotidesPf1N1B4_3271 and Pf1N1B4_3272 are separated by 19 nucleotidesPf1N1B4_3272 and Pf1N1B4_3273 are separated by 79 nucleotides Pf1N1B4_3269 - FIG00953317: hypothetical protein, at 3,658,930 to 3,659,949 _3269 Pf1N1B4_3270 - Alcohol dehydrogenase (EC 1.1.1.1), at 3,660,106 to 3,661,254 _3270 Pf1N1B4_3271 - Bicyclomycin resistance protein, at 3,661,251 to 3,662,417 _3271 Pf1N1B4_3272 - hypothetical protein, at 3,662,437 to 3,662,553 _3272 Pf1N1B4_3273 - Hydroxymethylglutaryl-CoA lyase (EC 4.1.3.4), at 3,662,633 to 3,662,893 _3273
Group Condition Pf1N1B4_3269 Pf1N1B4_3270 Pf1N1B4_3271 Pf1N1B4_3272 Pf1N1B4_3273
carbon source carbon source Sucrose 1mM -0.3 -0.5 +0.3 -1.1 -3.6
carbon source carbon source Sucrose 0.25mM +0.3 +0.1 -0.1 -0.8 -3.4
anaerobic Nitrate reduction +0.4 -0.2 -0.3 -2.4 +0.3
temperature LB 37C -0.0 -0.8 -1.2 -0.6 +0.4
stress Aluminum chloride 2.5 mM -0.2 -0.4 -0.4 -0.0 -1.2
pH Minimal media pH9 -0.4 +0.1 +0.3 -0.8 -1.1
carbon source carbon source Sucrose 0.5mM +0.2 -0.0 -0.7 -0.8 -0.3
nitrogen source nitrogen source nitrite 0.5mM +0.1 +0.2 -0.4 -0.4 -0.8
nitrogen source Thymine (N) +0.1 +0.5 -0.2 -0.5 -1.0
stress Cisplatin 0.006 mg/ml -0.2 +0.4 -0.6 -0.4 -0.4
stress Carbenicillin 2 mg/ml +0.0 -0.5 -0.8 +0.4 -0.0
carbon source D-Mannose (C) +0.1 -0.4 -0.1 +0.4 -0.8
nitrogen source Uridine (N) -0.1 +0.4 -0.1 +0.4 -1.4
temperature LB 37C -0.7 -0.3 -0.2 -0.5 +1.1
stress Chloride 200 mM +0.2 -0.1 -0.1 -1.0 +0.5
carbon source carbon source Sucrose 0.25mM +0.5 +0.0 -0.2 +0.0 -1.0
stress 1-ethyl-3-methylimidazolium lysine 5 mM -0.3 -0.1 +0.1 -0.8 +0.8
stress nitrite 10 mM -0.0 -0.3 -0.4 +1.0 -0.6
denitrifying Nitrate reduction, replicate A +0.1 +0.8 -0.1 -0.1 -1.0
carbon source D-Galactose (C) -0.1 +0.0 -0.2 -0.5 +1.0
nitrogen source nitrogen source nitrite 1mM +0.1 +0.3 -0.2 -0.5 +0.7
stress Cholin acetate 20 mM +0.2 +0.2 -0.3 -0.4 +0.9
denitrifying Nitrate reduction, replicate B t1 +0.2 +0.5 -0.1 +0.5 -0.4
nitrogen source D-Glucosamine Hydrochloride (N) +0.3 -0.3 -0.2 -0.4 +1.3
denitrifying Nitrate reduction, replicate C t3 +0.4 +0.8 +0.2 -0.6 +0.2
denitrifying Denitrifying in presence of WT Pseudomonas N2-E3; replicate B; timepoint 1 +0.0 +1.4 -0.1 -0.3 -0.1
carbon source carbon source Sucrose 5mM +0.1 +0.6 +0.2 -0.5 +0.6
denitrifying Nitrate reduction, replicate A t2 +0.4 +1.3 -0.1 -0.6 -0.0
stress Doxycycline hyclate 0.0001 mg/ml +0.1 +0.2 +0.2 +1.4 -0.7
denitrifying Denitrifying in presence of WT Pseudomonas N2-E3; replicate C; timepoint 1 +0.1 +1.0 -0.3 +0.5 +0.6
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