Fitness for 5 genes in Pseudomonas fluorescens FW300-N1B4

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500 ntPf1N1B4_3184 and Pf1N1B4_3185 are separated by 199 nucleotidesPf1N1B4_3185 and Pf1N1B4_3186 are separated by 5 nucleotidesPf1N1B4_3186 and Pf1N1B4_3187 are separated by 114 nucleotidesPf1N1B4_3187 and Pf1N1B4_3188 are separated by 4 nucleotides Pf1N1B4_3184 - Isochorismatase (EC 3.3.2.1), at 3,568,359 to 3,568,916 _3184 Pf1N1B4_3185 - Protein export cytoplasm protein SecA ATPase RNA helicase (TC 3.A.5.1.1), at 3,569,116 to 3,569,325 _3185 Pf1N1B4_3186 - Conserved secreted protein, at 3,569,331 to 3,569,816 _3186 Pf1N1B4_3187 - UPF0225 protein YchJ, at 3,569,931 to 3,570,407 _3187 Pf1N1B4_3188 - FIG00953202: hypothetical protein, at 3,570,412 to 3,570,909 _3188
Group Condition Pf1N1B4_3184 Pf1N1B4_3185 Pf1N1B4_3186 Pf1N1B4_3187 Pf1N1B4_3188
carbon source carbon source Sucrose 0.5mM -2.5 N.D. -0.4 -0.2 -0.3
carbon source carbon source Sucrose 0.25mM -2.1 N.D. +0.2 -0.2 -0.1
anaerobic Nitrate reduction -1.3 N.D. -0.2 +0.1 -0.3
anaerobic N2E2wt with N1B4m denitrifying repB -0.3 N.D. -0.4 +0.0 -0.9
pH Minimal media pH8 -1.1 N.D. -0.0 +0.1 -0.0
nitrogen source Thymine (N) -1.1 N.D. +0.3 -0.0 -0.3
carbon source octanoate (C) -0.8 N.D. +0.6 +0.1 -0.8
stress Thallium(I) acetate 0.008 mg/ml -1.0 N.D. -0.0 -0.2 +0.3
nitrogen source Cytidine (N) -0.5 N.D. +0.1 -0.1 -0.4
lb LB -1.1 N.D. -0.1 +0.1 +0.1
nitrogen source nitrogen source nitrite 0.5mM -0.5 N.D. -0.3 -0.2 +0.2
denitrifying Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate B -1.1 N.D. -0.0 +0.1 +0.1
nitrogen source nitrogen source nitrite 1mM -1.1 N.D. +0.1 -0.0 +0.2
stress Rifampicin 0.004 mg/ml -0.8 N.D. +0.0 +0.3 -0.1
temperature LB 37C -0.4 N.D. +0.3 -0.2 -0.3
nitrogen source Adenine (N) -0.6 N.D. +0.4 +0.1 -0.2
nitrogen source D-Glucosamine Hydrochloride (N) -1.0 N.D. +0.4 -0.2 +0.5
stress Cobalt chloride 0.3125 mM -0.7 N.D. +0.0 +0.2 +0.2
stress Paraquat dichloride 0.016 mg/mL -0.5 N.D. +0.0 -0.3 +0.4
nitrogen source Ammonium chloride (N) -0.6 N.D. +0.3 +0.2 -0.1
pH Minimal media pH9 -0.7 N.D. +0.4 +0.2 -0.1
denitrifying Nitrate reduction, replicate C +0.4 N.D. +0.1 +0.4 -1.0
nitrogen source L-Isoleucine (N) +0.4 N.D. +0.2 -0.1 -0.5
stress copper (II) chloride 2.5 mM +0.5 N.D. -0.3 +0.1 +0.2
stress Doxycycline hyclate 0.0001 mg/ml -0.4 N.D. +0.0 +0.6 +0.4
stress methylglyoxal 0.008 vol% +0.8 N.D. -0.2 +0.1 -0.1
stress Fusidic 0.125 mg/ml +1.0 N.D. +0.1 +0.2 -0.4
stress Choline Lysine 6 mM +1.1 N.D. -0.2 -0.1 +0.4
stress Gentamicin 0.001 mg/ml +0.6 N.D. +0.2 -0.0 +0.3
stress Aluminum chloride 2.5 mM +1.2 N.D. +0.1 +0.1 -0.0
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