Fitness for 5 genes in Pseudomonas fluorescens FW300-N1B4

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500 ntPf1N1B4_3002 and Pf1N1B4_3003 are separated by 372 nucleotidesPf1N1B4_3003 and Pf1N1B4_3004 are separated by 1045 nucleotidesPf1N1B4_3004 and Pf1N1B4_5216 are separated by 72 nucleotidesPf1N1B4_5216 and Pf1N1B4_3005 are separated by 107 nucleotides Pf1N1B4_3002 - hypothetical protein, at 3,375,873 to 3,376,031 _3002 Pf1N1B4_3003 - hypothetical protein, at 3,376,404 to 3,376,541 _3003 Pf1N1B4_3004 - Integrase, at 3,377,587 to 3,378,840 _3004 Pf1N1B4_5216 - tRNA-Leu-CAA, at 3,378,913 to 3,378,994 _5216 Pf1N1B4_3005 - S-adenosylmethionine:tRNA ribosyltransferase-isomerase (EC 5.-.-.-), at 3,379,102 to 3,380,166 _3005
Group Condition Pf1N1B4_3002 Pf1N1B4_3003 Pf1N1B4_3004 Pf1N1B4_5216 Pf1N1B4_3005
carbon source carbon source Sucrose 5mM -1.7 N.D. +0.2 N.D. -0.4
anaerobic N2E2wt with N1B4m denitrifying repA -1.1 N.D. -0.1 N.D. -0.6
nitrogen source Putrescine (N) -1.1 N.D. +0.0 N.D. -0.4
carbon source D-Trehalose (C) -0.8 N.D. -0.1 N.D. -0.6
carbon source L-Arginine (C) -0.7 N.D. -0.1 N.D. -0.5
stress Thallium(I) acetate 0.008 mg/ml +0.2 N.D. -0.0 N.D. -1.4
carbon source carbon source Sucrose 0.5mM -1.3 N.D. +0.3 N.D. +0.1
stress Rifampicin 0.004 mg/ml -1.1 N.D. -0.1 N.D. +0.3
stress Thallium(I) acetate 0.008 mg/ml +0.4 N.D. -0.1 N.D. -1.2
carbon source octanoate (C) -0.5 N.D. -0.4 N.D. +0.6
pH Minimal media pH9 -0.9 N.D. +0.3 N.D. +0.8
denitrifying Nitrate reduction, replicate C +0.7 N.D. +0.3 N.D. -0.7
denitrifying Denitrifying in presence of WT Pseudomonas N2-E3; replicate C; timepoint 1 +0.9 N.D. -0.2 N.D. -0.4
stress methylglyoxal 0.004 vol% -0.4 N.D. +0.0 N.D. +1.1
stress Fusidic 0.0625 mg/ml -1.4 N.D. -0.1 N.D. +2.1
denitrifying Nitrate reduction, replicate A +1.0 N.D. +0.3 N.D. -0.3
stress 1-ethyl-3-methylimidazolium lysine 5 mM +0.1 N.D. -0.2 N.D. +1.4
stress Choline chloride 200 mM -0.4 N.D. +0.2 N.D. +1.5
stress Nalidixic 0.004 mg/ml -0.6 N.D. -0.0 N.D. +1.9
stress nitrate 100 mM -0.3 N.D. +0.2 N.D. +1.5
stress Gentamicin 0.001 mg/ml -0.2 N.D. +0.3 N.D. +1.3
stress Benzethonium chloride 0.0125 mM +0.6 N.D. -0.1 N.D. +0.9
stress Doxycycline hyclate 0.0001 mg/ml -0.4 N.D. -0.3 N.D. +2.2
stress Chloride 200 mM +0.1 N.D. +0.3 N.D. +1.0
stress Sisomicin 0.001 mg/ml +0.1 N.D. +0.0 N.D. +1.4
stress 1-ethyl-3-methylimidazolium chloride 80 mM +0.0 N.D. +0.1 N.D. +1.5
stress Carbenicillin 2 mg/ml -0.1 N.D. -0.2 N.D. +2.4
stress Choline Lysine 6 mM +0.2 N.D. +0.2 N.D. +2.1
stress methylglyoxal 0.008 vol% +0.2 N.D. +0.2 N.D. +2.2
stress Fusidic 0.125 mg/ml +0.0 N.D. +0.2 N.D. +3.3
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