Fitness for 5 genes in Pseudomonas fluorescens FW300-N1B4

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500 ntPf1N1B4_298 and Pf1N1B4_299 are separated by 67 nucleotidesPf1N1B4_299 and Pf1N1B4_300 overlap by 4 nucleotidesPf1N1B4_300 and Pf1N1B4_301 are separated by 480 nucleotidesPf1N1B4_301 and Pf1N1B4_302 are separated by 259 nucleotides Pf1N1B4_298 - Transcription elongation factor GreB, at 352,571 to 353,044 _298 Pf1N1B4_299 - HIPA PROTEIN, at 353,112 to 354,389 _299 Pf1N1B4_300 - hypothetical protein, at 354,386 to 354,505 _300 Pf1N1B4_301 - FIG00957636: hypothetical protein, at 354,986 to 355,294 _301 Pf1N1B4_302 - Membrane protein, distant similarity to thiosulphate:quinone oxidoreductase DoxD, at 355,554 to 355,988 _302
Group Condition Pf1N1B4_298 Pf1N1B4_299 Pf1N1B4_300 Pf1N1B4_301 Pf1N1B4_302
stress Cholin acetate 20 mM -1.3 +0.1 N.D. N.D. -0.4
stress Carbenicillin 2 mg/ml -0.7 -0.3 N.D. N.D. -0.6
nitrogen source Cytidine (N) -0.9 -0.1 N.D. N.D. -0.5
carbon source carbon source Sucrose 0.5mM -2.0 +0.5 N.D. N.D. +0.0
phagefrs Time6-FRS -1.2 -0.0 N.D. N.D. -0.3
phagefrs Time16-FRS -0.9 -0.1 N.D. N.D. -0.4
stress 5-Hydroxymethylfurfural 2.5 mM -0.6 -0.1 N.D. N.D. -0.6
nitrogen source nitrogen source nitrite 0.1mM -0.7 -0.1 N.D. N.D. -0.3
nophagecontrol Time16-nophage -1.0 -0.2 N.D. N.D. +0.1
carbon source D-Galacturonic Acid (C) -0.8 -0.1 N.D. N.D. -0.1
nophagecontrol TIme6-nophage -1.3 -0.1 N.D. N.D. +0.3
stress Spectinomycin 0.025 mg/ml -1.1 +0.3 N.D. N.D. -0.1
denitrifying Nitrate reduction, replicate A t1 -0.4 +0.2 N.D. N.D. -0.6
pH Minimal media pH6 -1.0 +0.0 N.D. N.D. +0.2
carbon source Cytidine (C) -0.9 +0.0 N.D. N.D. +0.4
denitrifying Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate B -0.7 +0.0 N.D. N.D. +0.3
carbon source L-Leucine (C) -0.7 +0.0 N.D. N.D. +0.4
carbon source D-Trehalose (C) -0.6 -0.1 N.D. N.D. +0.4
stress Cisplatin 0.006 mg/ml -0.7 +0.5 N.D. N.D. -0.0
lb LB -0.6 +0.1 N.D. N.D. +0.4
stress Cephalothin 2.5 mg/ml -0.6 +0.0 N.D. N.D. +0.5
carbon source Putrescine (C) -0.3 -0.2 N.D. N.D. +0.6
stress methylglyoxal 0.008 vol% +0.6 +0.3 N.D. N.D. -0.7
denitrifying Denitrifying in presence of WT Pseudomonas N2-E3; replicate B; timepoint 1 +0.7 -0.1 N.D. N.D. -0.4
temperature LB 37C +0.8 -0.1 N.D. N.D. -0.3
carbon source carbon source Sucrose 1mM -0.4 +0.6 N.D. N.D. +0.5
stress Chloride 300 mM +0.8 +0.2 N.D. N.D. -0.1
stress 1-ethyl-3-methylimidazolium chloride 80 mM +0.7 +0.2 N.D. N.D. +0.3
stress Cisplatin 0.006 mg/ml +0.1 +0.4 N.D. N.D. +0.7
stress Fusidic 0.125 mg/ml +0.5 +0.4 N.D. N.D. +0.5
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