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Protein
Homologs
Fitness for 5 genes in
Pseudomonas fluorescens FW300-N1B4
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500 nt
Pf1N1B4_2838 and Pf1N1B4_2839 overlap by 4 nucleotides
Pf1N1B4_2839 and Pf1N1B4_2840 are separated by 8 nucleotides
Pf1N1B4_2840 and Pf1N1B4_2841 are separated by 1 nucleotides
Pf1N1B4_2841 and Pf1N1B4_2842 are separated by 2 nucleotides
Pf1N1B4_2838 - FIG003276: zinc-binding protein, at 3,183,580 to 3,183,780
_2838
Pf1N1B4_2839 - Dephospho-CoA kinase (EC 2.7.1.24), at 3,183,777 to 3,184,388
_2839
Pf1N1B4_2840 - Leader peptidase (Prepilin peptidase) (EC 3.4.23.43) / N-methyltransferase (EC 2.1.1.-), at 3,184,397 to 3,185,269
_2840
Pf1N1B4_2841 - Type IV fimbrial assembly protein PilC, at 3,185,271 to 3,186,488
_2841
Pf1N1B4_2842 - Type IV fimbrial assembly, ATPase PilB, at 3,186,491 to 3,188,191
_2842
Group
Condition
Pf1N1B4
_2838
Pf1N1B4
_2839
Pf1N1B4
_2840
Pf1N1B4
_2841
Pf1N1B4
_2842
carbon source
carbon source Sucrose 1mM
N.D.
N.D.
-1.2
-0.5
-0.2
denitrifying
Nitrate reduction, replicate C t3
N.D.
N.D.
-1.1
-0.3
-0.2
denitrifying
Nitrate reduction, replicate C t2
N.D.
N.D.
-1.1
-0.2
-0.3
denitrifying
Nitrate reduction, replicate A t2
N.D.
N.D.
-1.1
-0.2
-0.1
carbon source
carbon source Sucrose 0.5mM
N.D.
N.D.
-1.0
-0.1
-0.2
denitrifying
Nitrate reduction, replicate C
N.D.
N.D.
-1.2
-0.0
-0.1
denitrifying
Nitrate reduction, replicate B
N.D.
N.D.
-0.9
+0.0
-0.4
denitrifying
Nitrate reduction, replicate A
N.D.
N.D.
-1.3
+0.1
+0.0
denitrifying
Nitrate reduction, replicate B t2
N.D.
N.D.
-0.7
-0.2
-0.2
nitrogen source
nitrogen source nitrite 0.1mM
N.D.
N.D.
-0.6
-0.3
-0.1
temperature
LB 37C
N.D.
N.D.
-0.9
-0.2
+0.1
stress
Aluminum chloride 2.5 mM
N.D.
N.D.
-0.6
-0.1
-0.2
denitrifying
Nitrate reduction, replicate C t1
N.D.
N.D.
-0.6
-0.3
-0.1
anaerobic
Nitrate reduction
N.D.
N.D.
-0.6
-0.3
-0.0
nitrogen source
Uridine (N)
N.D.
N.D.
-0.7
-0.2
-0.0
stress
Rifampicin 0.004 mg/ml
N.D.
N.D.
-0.2
-0.4
-0.2
denitrifying
Denitrifying in presence of WT Pseudomonas N2-E3; replicate B; timepoint 1
N.D.
N.D.
-0.6
+0.1
-0.2
nitrogen source
Putrescine (N)
N.D.
N.D.
-0.5
-0.2
-0.0
carbon source
D-Trehalose (C)
N.D.
N.D.
-0.5
-0.1
-0.1
temperature
LB 20C
N.D.
N.D.
-0.6
-0.1
+0.1
carbon source
carbon source Sucrose 5mM
N.D.
N.D.
-0.5
-0.2
+0.2
stress
Tetracycline 0.0004 mg/ml
N.D.
N.D.
+0.3
-0.2
-0.3
stress
Bacitracin 2 mg/ml
N.D.
N.D.
+0.4
-0.0
-0.3
stress
nitrate 100 mM
N.D.
N.D.
+0.4
-0.2
-0.1
nitrogen source
nitrogen source nitrite 1mM
N.D.
N.D.
+0.5
-0.2
+0.0
nitrogen source
nitrogen source nitrite 0.1mM
N.D.
N.D.
+0.5
+0.0
-0.2
stress
Fusidic 0.0625 mg/ml
N.D.
N.D.
+0.4
-0.2
+0.3
carbon source
D-Ribose (C)
N.D.
N.D.
+0.7
-0.1
+0.0
stress
Chloride 300 mM
N.D.
N.D.
+0.4
+0.3
-0.1
carbon source
carbon source Sucrose 0.25mM
N.D.
N.D.
+0.7
+0.2
+0.0
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