Fitness for 5 genes in Pseudomonas fluorescens FW300-N1B4

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500 ntPf1N1B4_5202 and Pf1N1B4_5203 are separated by 12 nucleotidesPf1N1B4_5203 and Pf1N1B4_2825 are separated by 109 nucleotidesPf1N1B4_2825 and Pf1N1B4_2826 are separated by 340 nucleotidesPf1N1B4_2826 and Pf1N1B4_2827 are separated by 60 nucleotides Pf1N1B4_5202 - tRNA-Asn-GTT, at 3,169,907 to 3,169,979 _5202 Pf1N1B4_5203 - tRNA-Pro-TGG, at 3,169,992 to 3,170,065 _5203 Pf1N1B4_2825 - EF hand domain protein, at 3,170,175 to 3,170,576 _2825 Pf1N1B4_2826 - NADH dehydrogenase (EC 1.6.99.3), at 3,170,917 to 3,172,215 _2826 Pf1N1B4_2827 - FIG00953980: hypothetical protein, at 3,172,276 to 3,172,449 _2827
Group Condition Pf1N1B4_5202 Pf1N1B4_5203 Pf1N1B4_2825 Pf1N1B4_2826 Pf1N1B4_2827
carbon source carbon source Sucrose 1mM N.D. N.D. -1.8 -0.2 -0.2
carbon source carbon source Sucrose 1mM N.D. N.D. -0.8 +0.1 -1.1
stress Nalidixic 0.004 mg/ml N.D. N.D. -0.4 -0.2 -0.7
carbon source Fumarate (C) N.D. N.D. -0.3 -0.0 -0.9
carbon source carbon source Sucrose 0.25mM N.D. N.D. +0.1 -0.8 -0.4
lb LB N.D. N.D. -0.2 -0.1 -0.8
carbon source carbon source Sucrose 0.25mM N.D. N.D. -0.1 +0.4 -1.3
denitrifying Denitrifying in presence of WT Pseudomonas N2-E3; replicate C; timepoint 1 N.D. N.D. -0.7 +0.1 -0.4
pH Minimal media pH9 N.D. N.D. -0.6 -0.4 +0.1
nitrogen source nitrate (N) N.D. N.D. +0.3 -0.3 -0.6
nitrogen source Cytidine (N) N.D. N.D. +0.3 -0.1 -0.8
carbon source D,L-Lactate (C) N.D. N.D. +0.2 -0.3 -0.5
denitrifying Denitrifying in presence of WT Pseudomonas N2-E3; replicate B; timepoint 1 N.D. N.D. -0.9 -0.0 +0.5
denitrifying Nitrate reduction, replicate C N.D. N.D. -0.6 -0.1 +0.3
nitrogen source nitrogen source nitrite 0.1mM N.D. N.D. +0.8 -0.6 -0.6
stress nitrite 10 mM N.D. N.D. +0.0 +0.4 -0.8
stress Doxycycline hyclate 0.0001 mg/ml N.D. N.D. -0.6 +0.3 -0.1
carbon source D-Lactate (C) N.D. N.D. +0.3 +0.1 -0.8
carbon source carbon source Sucrose 5mM N.D. N.D. +0.8 +0.3 -1.3
nitrogen source Uridine (N) N.D. N.D. +0.5 -0.0 -0.6
nitrogen source nitrogen source Ammonium chloride 20mM N.D. N.D. +0.5 -0.1 -0.5
anaerobic Nitrate reduction N.D. N.D. -0.7 +0.2 +0.5
carbon source carbon source Sucrose 5mM N.D. N.D. -0.2 -0.3 +0.5
carbon source D-Gluconic Acid (C) N.D. N.D. +0.5 -0.4 +0.1
nitrogen source D-Glucosamine Hydrochloride (N) N.D. N.D. +0.6 -0.0 -0.4
denitrifying Nitrate reduction, replicate C t2 N.D. N.D. +0.5 +0.1 -0.3
denitrifying Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate A N.D. N.D. +0.6 +0.2 -0.3
nitrogen source nitrogen source nitrate 20mM N.D. N.D. +1.0 +0.1 -0.2
nitrogen source nitrogen source nitrate 5mM N.D. N.D. +0.8 +0.0 +0.4
temperature LB 37C N.D. N.D. +0.0 +0.5 +0.8
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